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产品信息
17-6979-42
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
VIM3b
偶联物
激发/发射光谱
651/660 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with 0.2% BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_10548515
产品详细信息
Description: This VIM3b monoclonal antibody reacts with human CD97, a member of the epidermal growth factor (EGF)-TM7 family of cell surface receptors. Present on a variety of hematopoietic cells, CD97 is most highly expressed on granulocytes and monocytes. CD97 can also be detected on lymphocytes, with T and NK cells expressing higher levels than B cells. Moreover, CD97 expression on T lymphocytes can be induced by stimulation of CD3 and CD28. Studies have demonstrated that CD97 is involved in granulocyte trafficking and T cell activation. CD55, also known as Decay-accelerating factor (DAF), is the ligand of CD97.^M
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Due to alternative splicing, CD97 exists as three isoforms with molecular weights of 74, 80, and 86 kDa. The VIM3b monoclonal antibody recognizes each form.^M
^M
Applications Reported: This VIM3b antibody has been reported for use in flow cytometric analysis.^M
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Applications Tested: This VIM3b antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.^M
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Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.^M
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Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
CD97 is a G-protein-coupled seven-span transmembrane adhesive receptor that is constitutively expressed on granulocytes and monocytes and rapidly upregulated on T and B cells upon activation. CD97 is produced in alternatively spliced forms and its cellular ligand is CD55 (DAF), which protects various cell types from complement-mediated damage. Interaction of CD97 on leukocytes and CD55 on vessel cells probably facilitate leukocyte activation and migration into the tissues, similarly, CD97 seems to play a role in tumour migration and invasiveness. CD97 is involved in T cell regulation and peripheral granulocyte homeostasis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: Adhesion G protein-coupled receptor E5; CD97; CD97 molecule; Leukocyte antigen CD97; seven transmembrane helix receptor; seven-span transmembrane protein; seven-transmembrane, heterodimeric receptor associated with inflammation
基因别名: ADGRE5; CD97; TM7LN1
UniProt ID: (Human) P48960
Entrez Gene ID: (Human) 976
