Invitrogen

CDK2 Polyclonal Antibody

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CDK2 Antibody in Western Blot (WB) — Western Blot using CDK2 Polyclonal Antibody (Product # PA5-80459) at 1:500 dilution. Lane A: K562 Whole Cell Lysate. Lysates/proteins at 60 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 30 kDa.

CDK2 Antibody in Immunoprecipitation (IP) — CDK2 Immunoprecipitation using: Lane A: 0.5 mg K562 Whole Cell Lysate 2 µL with CDK2 Polyclonal Antibody (Product # PA5-80459) and 15 µL of 50 % Protein G agarose. Primary antibody: CDK2 Polyclonal Antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 34 kDa.

产品信息

PA5-80459

应用

建议稀释比

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免疫印迹 (WB)

1:500-1:2,000

酶联免疫吸附实验 (ELISA)

1:1,000-1:2,000

免疫沉淀 (IP)

2-4 µL/mg of lysate

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant Human CDK2 protein (Met1-Leu298)

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偶联物

Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

RRID

AB_2787773

产品详细信息

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

This antibody has specificity for Human CDK2/p33.

靶标信息

p33cdk2 associates with cyclin A in human cells. Kinase activity associated with cyclin A-cdc2 is found only in G2-phase. Cdk2 also complexes with cyclins E, D1, and D3. Cyclin E-cdk2 kinase is active in the G1- and S-phases of the cell cycle and is important (as does cyclin A-cdk2) for the progression from G1-to S-phase. The levels of cyclin A-cdk2 is maximal at the G1/S transition and both cdk2 and cyclin A are found associated with DNA in the initiation complex during replication. Rb protein acts as substrate for cdk2-cyclin E and/or cdk2-cyclin A in vivo. Cdk2 is activated and specifically localized to the nucleus during late G1-, S-, and G2-phase.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: cdc2-related protein kinase; CDC28; CDC2A; Cell division protein kinase 2; Cyclin-dependent kinase 2; EC 2.7.11.22; EC 2.7.11.23; kinase Cdc2; p33 protein kinase; p33CDK2

基因别名: CDK2; CDKN2; p33(CDK2)

UniProt ID: (Human) P24941

Entrez Gene ID: (Human) 1017

分子生物学功能: non-receptor serine/threonine protein kinase