Invitrogen

CDK4 Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2个已发表图片

2 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

CDK4 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 14

CDK4 Antibody (PA5-27827) in ICC/IF

Immunofluorescence analysis of CDK4 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CDK4 Rabbit Polyclonal Antibody(Product # PA5-27827) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) ... View More

CDK4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

CDK4 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A-431 cells were transfected with CDK4 siRNA and loss of signal was observed in Western Blot using Anti-CDK4 Polyclonal Antibody (Product # PA5-27827). {KD}

产品信息

PA5-27827

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Bovine, Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 148 and 303 of Human CDK4

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.82 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2545303

产品详细信息

Recommended positive controls: 293T, A431, HeLa, HepG2, NIH-3T3, PC-12.

Predicted reactivity: Mouse (95%), Rat (94%), Pig (98%), Sheep (96%), Rhesus Monkey (100%), Bovine (96%).

靶标信息

CDK4/cyclin D3 serine/threonine kinase is dimeric protein complex. CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of cell cycle. CDK4 forms a complex with the D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 can mediate phosphorylation of the C-terminal region of Rb protein leading to an active transcriptional repression of E2F complex. CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivity in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Effects of lncRNA ANRIL-knockdown on the proliferation, apoptosis and cell cycle of gastric cancer cells.

Oncology letters

Hu X,Lou T,Yuan C,Wang Y,Tu X,Wang Y,Zhang T

Published figure using CDK4 polyclonal antibody (Product # PA5-27827) in Western Blot

Sun Aug 01 00:00:00 EDT 2021

Effects of lncRNA ANRIL-knockdown on the proliferation, apoptosis and cell cycle of gastric cancer cells.

Oncology letters

Hu X,Lou T,Yuan C,Wang Y,Tu X,Wang Y,Zhang T

Published figure using CDK4 polyclonal antibody (Product # PA5-27827) in Western Blot

Sun Aug 01 00:00:00 EDT 2021

DNA damage in embryonic neural stem cell determines FTLDs' fate via early-stage neuronal necrosis.

Life science alliance

Homma H,Tanaka H,Jin M,Jin X,Huang Y,Yoshioka Y,Bertens CJ,Tsumaki K,Kondo K,Shiwaku H,Tagawa K,Akatsu H,Atsuta N,Katsuno M,Furukawa K,Ishiki A,Waragai M,Ohtomo G,Iwata A,Yokota T,Inoue H,Arai H,Sobue G,Sone M,Fujita K,Okazawa H

PA5-27827 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to study developmental stress followed by early-stage neuronal necrosis is a potential target for therapeutics and one of the earliest general biomarkers for FTLD.

Thu Jul 01 00:00:00 EDT 2021

生物信息学

蛋白别名: Cell division protein kinase 4; CRK3; cyclin dependent kinase 4; Cyclin-dependent kinase 4; MGC14458; p34/cdk4; PSK-J3; serine/threonine kinase

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基因别名: CDK4; CMM3; Crk3; PSK-J3