Invitrogen

CDK8 Polyclonal Antibody

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

2个已发表图片

3篇引用文献

查看其他15个CDK8抗体

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CDK8 Antibody in ChIP assay (ChIP) — Chromatin immunoprecipitation analysis of Cdk8 was performed using cross-linked chromatin from 1 x 106 HCT116 colon carcinoma cells treated with serum for 0, 15, 30, and 60 minutes. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (see reference for Matrix ChIP protocol: http://www.ncbi.nlm.nih.gov/pubmed/22098709) with 1.0 µL/100 µL well volume of a Cdk8 polyclonal antibody (Product # PA1-21780). Chromatin aliquots from ~1 x 105 cells were used per ChIP pull-down. Quantitative PCR data were done in quadruplicate using 1 µL of eluted DNA in 2 µL SYBR real-time PCR reactions containing primers to amplify -15kb upstream of the Egr1 gene or exon-1 of Egr1. PCR calibration curves were generated for each primer pair from a dilution series of sheared total genomic DNA. Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM for three experiments. A schematic representation of the Egr-1 locus is shown above the data where boxes represent exons (black boxes = translated regions, white boxes = untranslated regions); the zigzag line represents an intron; and the straight line represents upstream sequence. Regions amplified by Egr-1 primers are represented by black bars. Data courtesy of the Innovators Program.

CDK8 Antibody — Figure 6. Transcriptional memory leads to Ssn3/Cdk8-dependent poised preinitiation complex. ( A ) ChIP against Med1-GFP, Med13-GFP or Ssn8-GFP from cells grown under repressing, activating or memory conditions. ( B ) ChIP against Ssn3-FRB-GFP from cells grown in repressing, activating or memory conditions. ( A and B ) *p<0.05, compared with the repressing condition (Student's t-test). ( C ) ChIP against RNAPII from strains expressing Ssn3-FRB-GFP grown under either repressing or memory conditions, before or after treatment 1 ug/ml of rapamycin using primers to amplify the INO1 promoter (-348 to -260) or the PRM1 CDS. Inset: confocal micrographs of Ssn3-FRB-GFP expressing cells before or after treatment with 1 mg/ml of rapamycin for 30 min. *p<0.05, compared with t = 0 (Student's t-test). ( D and E ) INO1 activation ( D ) or reactivation ( E ) in Ssn3-FRB-GFP cells. For activation at time = 0, cells were shifted from medium containing 100 muM inositol (repressing conditions; red arrow in schematic) to medium without inositol (activating conditions; green arrow in schematic). For reactivation, cells were shifted from activating medium to repressing medium containing 100 muM inositol for 3 hr. Cells were treated +-1 ug/ml rapamycin for 45 min before transferring to activating conditions. Cells were harvested at the indicated time points, and INO1 mRNA levels were quantified relative to ACT1 mRNA levels by RT-qPCR. The averages of three biological replicates are shown +- standard {TM}

CDK8 Antibody in Immunocytochemistry, Proximity Ligation Assay (PLA) (ICC/IF, PLA) — Figure 4 PLA Analysis of the Interaction of STAT3 and CDK8/9 Induced upon HyIL-6 Stimulation in Human Primary CD4 + Th-1 Cells (A and B) Kinetics of the STAT3/CDK8 (A) or STAT3/CDK9 (B) interaction induced by 20 nM HyIL-6 in human primary CD4 + Th-1 cells. Scale bars, 20 mum. Statistical significance was calculated by one-way ANOVA. (C and D) STAT3/CDK8 (C) or STAT3/CDK9 (D) interactions were analyzed by PLA upon 20 nM HyIL-6 stimulation in the absence or presence of 2 muM MSC2530818 or 2 muM flavopiridol or upon treatment with the inhibitor only. Scale bars, 20 mum. Statistical significance was calculated by unpaired t test. White arrows in A to D indicate examples of cells where interaction signal was detected. Cumulative plots from n = 15 pictures alongside show the percentage of positive cells. Error bars show mean +- SEM. The p values were calculated based on non-parametric two-tailed Wilcoxon rank-sum test against the control group (first bar on the left). (E) STAT3/CDK9 interaction analyzed by PLA upon 20 nM HyIL-6 stimulation in STAT3 KnD Hut78 cells reconstituted with STAT3 WT-GFP (top panel) or STAT3 S727A-GFP (bottom panels). White arrows indicate examples of cells expressing the recombinant protein and where the STAT3/CDK9 interaction was detected by PLA. Scale bars, 20 mum. Graphs alongside show the nuclear GFP MFI normalized to unstimulated cells (top graph) or the nuclear STAT3/CDK9 PLA MFI in GFP-positive cells normalized to unstimulated cells (bottom graph).

产品信息

PA1-21780

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1-2 µg/mL	-
免疫细胞化学 (ICC/IF)	Assay-dependent	查看1篇已发表文章 1篇已发表文章
免疫沉淀 (IP)	10 µg/mg of lysate	-
染色质免疫沉淀实验 (ChIP)	1-3 µL	查看1篇已发表文章 1篇已发表文章
in situ PLA (PLA)	-	查看1篇已发表文章 1篇已发表文章

产品规格
种属反应	Human, Mouse, Rat
已发表种属	Human
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Synthetic peptide: C-SQQPPQYSHQTHRY, corresponding to amino acids 451-464 of Human Cdk8.
偶联物	Unconjugated
形式	Liquid
浓度	0.2 mg/mL
纯化类型	Protein A
保存液	10mM PBS, pH 7.4, with 0.2% BSA
内含物	0.09% sodium azide
保存条件	4° C, do not freeze
RRID	AB_2291488

产品详细信息

Recommended positive controls: HT29 cells.

靶标信息

A family of proteins designated as cdks are critical regulators of cell cycle progression. The prototype member of this family, p34/Cdc 2, and a related protein Cdk 2, function late in the cycle while Cdk 4 and Cdk 6 are critically involved in G1 to S progression. The p54/Cdk 8 interacts exclusively with Cyclin C. The Cdk 8 Cyclin C complex activates the kinase. Because of analogy with yeast Cdk 8, it has been proposed that the human Cdk 8 Cyclin C may interact with RNA polymerase, thus influencing the transcription mechanism(s) that lead to cell division.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

CDK8 Fine-Tunes IL-6 Transcriptional Activities by Limiting STAT3 Resident Time at the Gene Loci.

Cell reports

Martinez-Fabregas J,Wang L,Pohler E,Cozzani A,Wilmes S,Kazemian M,Mitra S,Moraga I

PA1-21780 was used in Proximity Ligation Assay (PLA) to investigate the ability of the cytokine CDK8 to regulate the immune response using interleukin-6 as a model system.

Tue Dec 22 00:00:00 EST 2020

CDK8 Fine-Tunes IL-6 Transcriptional Activities by Limiting STAT3 Resident Time at the Gene Loci.

Cell reports

Martinez-Fabregas J,Wang L,Pohler E,Cozzani A,Wilmes S,Kazemian M,Mitra S,Moraga I

PA1-21780 was used in Proximity Ligation Assay (PLA) to investigate the ability of the cytokine CDK8 to regulate the immune response using interleukin-6 as a model system.

Tue Dec 22 00:00:00 EST 2020

CDK8 Fine-Tunes IL-6 Transcriptional Activities by Limiting STAT3 Resident Time at the Gene Loci.

Cell reports

Martinez-Fabregas J,Wang L,Pohler E,Cozzani A,Wilmes S,Kazemian M,Mitra S,Moraga I

PA1-21780 was used in Proximity Ligation Assay (PLA) to investigate the ability of the cytokine CDK8 to regulate the immune response using interleukin-6 as a model system.

Tue Dec 22 00:00:00 EST 2020

Set1/COMPASS and Mediator are repurposed to promote epigenetic transcriptional memory.

eLife

D'Urso A,Takahashi YH,Xiong B,Marone J,Coukos R,Randise-Hinchliff C,Wang JP,Shilatifard A,Brickner JH

Published figure using CDK8 polyclonal antibody (Product # PA1-21780) in ChIP assay

Thu Jun 23 00:00:00 EDT 2016

生物信息学

蛋白别名: CDK8 protein kinase; Cell division protein kinase 8; Cyclin-dependent kinase 8; Mediator complex subunit CDK8; Mediator of RNA polymerase II transcription subunit CDK8; MGC126074; MGC126075; Protein kinase K35

基因别名: CDK8; K35; RGD1560888

UniProt ID: (Human) P49336, (Mouse) Q8R3L8

Entrez Gene ID: (Human) 1024, (Mouse) 264064, (Rat) 498140

分子生物学功能: non-receptor serine/threonine protein kinase

功能

transcription initiation from RNA polymerase II promoter protein phosphorylation positive regulation of transcription from RNA polymerase II promoter regulation of cell cycle transcription, DNA-templated regulation of transcription, DNA-templated phosphorylation protein ubiquitination negative regulation of triglyceride metabolic process

参与通路

protein kinase activity protein serine/threonine kinase activity cyclin-dependent protein serine/threonine kinase activity protein binding ATP binding RNA polymerase II carboxy-terminal domain kinase activity nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups ubiquitin protein ligase activity

CDK8 Polyclonal Antibody

产品信息

PA1-21780

免疫印迹 (WB)

免疫细胞化学 (ICC/IF)

免疫沉淀 (IP)

染色质免疫沉淀实验 (ChIP)

in situ PLA (PLA)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

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CDK8 Polyclonal Antibody

产品信息

PA1-21780

免疫印迹 (WB)

免疫细胞化学 (ICC/IF)

免疫沉淀 (IP)

染色质免疫沉淀 实验 (ChIP)

in situ PLA (PLA)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Brands

Shopping Tools

eSolutions

Support

我们的公司

Your items have has been added!

染色质免疫沉淀实验 (ChIP)