Rockland

CENPO Polyclonal Antibody

Name: CENPO Polyclonal Antibody
Brand: Rockland
SKU: 600-401-AH5
Availability: InStock

查看其他3个CENPO抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (1)

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CENPO Antibody (600-401-AH5) in WB

Western Blot of CENPO antibody. Lane 1: Mouse kidney tissue lysate with CENPO antibody at 1 µg/mL. Lane 2: Mouse kidney tissue lysate with CENPO antibody at 2 µg/mL. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 34 kDa, 30 kDa for CENPO. Other band(s): CENPO splice variants and isoforms.

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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产品信息

600-401-AH5

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

酶联免疫吸附实验 (ELISA)

1:10,000-1:20,000

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Anti-CENPO antibody was prepared from whole rabbit serum produced by repeated immunizations with a 16 amino acid synthetic peptide from near the internal region of human CENPO.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.02% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

Accurate chromosome segregation during mitosis requires a kinetochore to assemble correctly at the centromere of each chromatid and form a dynamic interface with the microtubules of the mitotic spindle. The kinetochore assembly includes the multisubunit CENP-H/I complex which can be divided into three functional classes based on phenotype analysis. One of these classes is referred to as the CENP-O class of proteins of which CENPO is the exemplar. These proteins form a stable complex with each other and are required for proper kinetochore function and for recovery from mitotic spindle damage. CENPO has also been shown to be an auto-antigen in a small population of anti-centromere antibodies- (ACA-) positive patients with sclerodoma.

仅用于科研。不用于诊断过程。未经明确授权不得转售。