Invitrogen

CEP95 Polyclonal Antibody

查看其他4个CEP95抗体

问题与答案

问题与答案

问题与答案

Additional Information:

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抗体检测数据 (2)

CEP95 Antibody in Immunocytochemistry (ICC/IF)

CEP95 Antibody in Immunocytochemistry (ICC/IF)

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CEP95 Antibody (PA5-101534) in ICC/IF

Immunocytochemistry analysis of CEP95 in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with CEP95 polyclonal antibody (Product # PA5-101534) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

CEP95 Antibody in Immunocytochemistry (ICC/IF)

CEP95 Antibody in Western Blot (WB)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

CEP95 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of CEP95 in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with CEP95 polyclonal antibody (Product # PA5-101534) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

CEP95 Antibody in Western Blot (WB) — Western blot analysis of CEP95 in HUVEC cell lysate (left lane: treated with the antigen-specific peptide). Samples were incubated with CEP95 polyclonal antibody (Product # PA5-101534).

Immunogen image

CEP95 Polyclonal Antibody

Immunogen Image CEP95 Polyclonal Antibody

产品信息

PA5-101534

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human CEP95(Accession Q96GE4), corresponding to amino acid residues Q590-Q640.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody detects endogenous levels of total CCDC45.

靶标信息

CEP95 gene ontology (GO) annotation includes protein binding and cytoskeleton.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Centrosomal protein of 95 kDa; Cep95; coiled-coil domain containing 45; Coiled-coil domain-containing protein 45

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基因别名: 4732496G21Rik; AI118346; AI448335; CCDC45; CEP45; CEP95; D330027A14Rik; F630025I20Rik; RGD1309655

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UniProt ID: (Mouse) Q8BVV7, (Rat) Q5XI03

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Entrez Gene ID: (Mouse) 320162, (Rat) 287766

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molecular_function

biological_process