Invitrogen

CLPP Polyclonal Antibody

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CLPP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of CLPP on paraffin-embedded human liver cancer tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with CLPP polyclonal antibody (Product# PA5-79051) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Development was performed using Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.

CLPP Antibody in Western Blot (WB) — Western blot analysis of CLPP in Lane 1: human A375 whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with CLPP polyclonal antibody (Product # PA5-79051) at a dilution of 0.5 µg/mL (overnight at 4°C), followed by goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10,000. Signal development was performed using a chemiluminescence (ECL) kit.

产品信息

PA5-79051

应用	建议稀释比	已发表文章
免疫印迹 (WB)	0.1-0.5 µg/mL	查看1篇已发表文章 1篇已发表文章
免疫组化（石蜡） (IHC (P))	0.5-1 µg/mL	-
免疫组化（冰冻） (IHC (F))	0.5-1 µg/mL	-
免疫细胞化学 (ICC/IF)	2 µg/mL	-
流式细胞分析 (Flow)	1-3 µg/1x10^6 cells	-

产品规格
种属反应	Human, Mouse, Rat
已发表种属	Mouse
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	E. coli-derived human CLPP recombinant protein (Position: A184-H247).
偶联物	Unconjugated
形式	Lyophilized
浓度	500 µg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS with 4mg trehalose
内含物	0.05mg sodium azide
保存条件	-20°C
RRID	AB_2746167

产品详细信息

Add 0.2 mL of distilled water, will yield a concentration of 500 µg/mL.

靶标信息

The protein encoded by this gene belongs to the peptidase family S14 and hydrolyzes proteins into small peptides in the presence of ATP and magnesium. The protein is transported into mitochondrial matrix and is associated with the inner mitochondrial membrane.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

ALKBH7 mediates necrosis via rewiring of glyoxal metabolism.

eLife

Kulkarni CA,Nadtochiy SM,Kennedy L,Zhang J,Chhim S,Alwaseem H,Murphy E,Fu D,Brookes PS

PA5-79051 was used in Western Blotting to show ALKBH7 regulates dialdehyde metabolism, which impacts the cardiac response to ischemia-reperfusion (IR) injury.

Fri Aug 14 00:00:00 EDT 2020

生物信息学

蛋白别名: ATP-dependent Clp protease proteolytic subunit, mitochondrial; ATP-dependent protease ClpAP, proteolytic subunit, human; caseinolytic mitochondrial matrix peptidase proteolytic subunit; caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog; caseinolytic protease, ATP-dependent, proteolytic subunit homolog; ClpP caseinolytic peptidase ATP-dependent, proteolytic subunit; ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit; ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog; ClpP caseinolytic protease, ATP-dependent, proteolytic subunit homolog; Endopeptidase Clp; putative ATP-dependent Clp protease proteolytic subunit, mitochondrial

基因别名: AU019820; CLPP; D17Wsu160e; DFNB81; PRLTS3

UniProt ID: (Human) Q16740, (Mouse) O88696

Entrez Gene ID: (Human) 8192, (Rat) 301117, (Mouse) 53895

分子生物学功能: serine protease