Invitrogen

CLPP Polyclonal Antibody

CLPP Antibody in Immunocytochemistry (ICC/IF)

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CLPP Antibody (PA5-79051) in ICC/IF

Immunocytochemistry analysis of CLPP using anti-CLPP antibody (Product # PA5-79051) . CLPP was detected in a section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-CLPP antibody (Product # PA5-79051) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visu... View More

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CLPP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of CLPP in paraffin-embedded section of human gallbladder adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution).The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-CLPP antibody (Product # PA5-79051) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

CLPP Antibody in Western Blot (WB) — Western blot analysis of CLPP in Lane 1: human A375 whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with CLPP polyclonal antibody (Product # PA5-79051) at a dilution of 0.5 µg/mL (overnight at 4°C), followed by goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10,000. Signal development was performed using a chemiluminescence (ECL) kit.

CLPP Antibody in Flow Cytometry (Flow) — Flow Cytometry of CLPP in U937 cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with CLPP Polyclonal Antibody (Product # PA5-79051) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.

产品信息

PA5-79051

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

2 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E. coli-derived human CLPP recombinant protein (Position: A184-H247).

查看免疫原

偶联物

Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

-20°C

RRID

AB_2746167

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

靶标信息

The protein encoded by this gene belongs to the peptidase family S14 and hydrolyzes proteins into small peptides in the presence of ATP and magnesium. The protein is transported into mitochondrial matrix and is associated with the inner mitochondrial membrane.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

ALKBH7 mediates necrosis via rewiring of glyoxal metabolism.

eLife

Kulkarni CA,Nadtochiy SM,Kennedy L,Zhang J,Chhim S,Alwaseem H,Murphy E,Fu D,Brookes PS

PA5-79051 was used in Western Blotting to show ALKBH7 regulates dialdehyde metabolism, which impacts the cardiac response to ischemia-reperfusion (IR) injury.

Fri Aug 14 00:00:00 EDT 2020

生物信息学

蛋白别名: ATP-dependent Clp protease proteolytic subunit, mitochondrial; ATP-dependent protease ClpAP, proteolytic subunit, human; caseinolytic mitochondrial matrix peptidase proteolytic subunit; caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog; caseinolytic protease, ATP-dependent, proteolytic subunit homolog; ClpP caseinolytic peptidase ATP-dependent, proteolytic subunit; ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit; ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog; ClpP caseinolytic protease, ATP-dependent, proteolytic subunit homolog; Endopeptidase Clp; putative ATP-dependent Clp protease proteolytic subunit, mitochondrial

基因别名: AU019820; CLPP; D17Wsu160e; DFNB81; PRLTS3

UniProt ID: (Human) Q16740, (Mouse) O88696

Entrez Gene ID: (Human) 8192, (Rat) 301117, (Mouse) 53895

分子生物学功能: serine protease