MA1-40276 detects CNF1/CNF2 from E. coli samples.
MA1-40276 has been successfully used in ELISA, functional assays and Western blot applications.
The MA1-40276 immunogen is residues 169-191 of N-terminus of CNF1.
Endotoxin <24 EU/mg.
Cytotoxic necrotizing factor type 1 and 2 (CNF1 & CNF2) belong to a family of bacterial toxins that target the small GTP-binding Rho proteins that regulate the actin cytoskeleton. Members of this toxin family typically inactivate Rho; however, CNF1 and the highly related CNF2 activate Rho by deamidation. CNF1 is more frequently associated with E.coli strains that cause extraintestitinal infections in humans, particularly those of the urinary tract (such as cystitis, pyelonephritis and prostatitis). In CNF1-producing uropathogenic E. coli strains, CNF1 is chromosomally encoded and typically resides on a pathogenicity island that also contains hemolysin and P fimbria- related genes. Both CNF1 and the highly related, plasmid-encoded CNF2 are monomeric, cytoplasmic toxins of approximately 115 kDa. CNF1 can be structurally organized into three functional domains the N-terminal, central and the C-terminal domain. The latter exhibits the catalytic activity of the toxin.
蛋白别名: Cytotoxic necrotizing factor 1; Cytotoxic necrotizing factor 2