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Agrisera
CNfu1/NifU-like protein 1 (chloroplastic) Polyclonal Antibody
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CNfu1/NifU-like protein 1 (chloroplastic) Antibody (AS13-2642) in WB
3 µg of total protein from Arabidopsis thaliana extracted with were separated on 15% SDS-PAGE and blotted 1 h to PVDF. Blots were blocked with 10% skimmed milk for 1 h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1,000 overnight at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed twice for 15 m... View More
产品信息
AS13-2642
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
Recombinant NFU1 derived from Arabidopsis thaliana NFU1 sequence.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
CNfu1 encodes a NifU-like iron-sulfur (Fe-S) cluster scaffold/transfer factor that functions in mitochondria, consistent with an N-terminal mitochondrial targeting sequence and enrichment within the mitochondrial matrix where Fe-S cluster biogenesis and delivery occur. The predicted protein architecture is characteristic of NFU-family factors, comprising an N-terminal NFU_N region followed by a C-terminal NifU-like domain that harbors the conserved cysteine motifs required for transient coordination of Fe-S clusters, enabling the protein to act as an intermediate carrier during late steps of Fe-S protein maturation. Biologically, CNfu1 supports the assembly and/or transfer of 4Fe-4S clusters to a subset of mitochondrial client apoproteins, notably including components of respiratory chain complex II (succinate dehydrogenase) and lipoic acid synthase, thereby linking CNfu1 activity to oxidative phosphorylation capacity and mitochondrial protein lipoylation-dependent pathways. Through this role, CNfu1 contributes to core mitochondrial metabolism by ensuring proper maturation of Fe-S-dependent enzymes and maintaining cellular energy and redox homeostasis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AnfU-IVb; CNfu1; NFU1
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