Invitrogen

COL11A1 Recombinant Rabbit Monoclonal Antibody (JE40-84)

Name: COL11A1 Recombinant Rabbit Monoclonal Antibody (JE40-84)
Brand: Invitrogen
SKU: MA562908

高级验证

查看其他7个COL11A1抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (4)
高级验证 (1)

COL11A1 Antibody in Immunocytochemistry (ICC/IF)

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COL11A1 Antibody (MA562908) in ICC/IF

Immunofluorescence analysis of COL11A1 was performed using Saos-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with COL11A1 Recombinant Rabbit Monoclonal Antibody (JE40-84) (Product # MA562908) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2,000 dilution), for 45 minutes at room t... View More

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Immunogen image

Immunogen Image COL11A1 Recombinant Rabbit Monoclonal Antibody (JE40-84)

产品信息

MA562908

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JE40-84

抗原

Recombinant protein within Human COL11A1 aa 501-600/1,806.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 0.05% BSA, 40% glycerol

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Positive Control: K-562 cell lysate, Jurkat cell lysate, PANC-1 cell lysate, HCT 116 cell lysate.

Tissue Specificity: Tissue enhanced (placenta, retina).

Subcellular Location: Secreted, extracellular space, extracellular matrix.

Sequence Similarities: 97% Mouse/Rat.

Predicted band size: 181 kDa

靶标信息

Collagen Type XI is an 1806 amino acid protein belonging to the fibrillar collagen family. Collagen Type XI is thought to play an important role in fibrillogenesis by controlling lateral growth of collagen II fibrils. This protein forms trimers composed of three different chains: a 1(XI), a 2(XI), and a 3(XI). a 3(XI) is a post-translational modification of a 1(II). a 1(V) can also be found instead of a 3(XI). Collagen Type XI has three named isoforms (A,B,C) and additional isoforms seem to exist, stemming from alternative usage of exon IIA or exon IIB. Transcripts containing exon IIA or IIB are present in cartilage, but exon IIB is preferentially utilized in transcripts from tendon. Collagen Type XI contains a single collagen binding TSP N-terminal (TSPN) domain. Collagen Type XI is expressed in cartilage, placenta and some tumor or virally transformed cell lines. Isoform expression can be tissue specific. Defects in the COL11A gene are the cause of Stickler syndrome type 2 (STL2), or beaded vitreous type, due to the presence of irregularly thickened fiber bundles throughout vitreous cavity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。