Invitrogen

COL20A1 Polyclonal Antibody

查看其他4个COL20A1抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (4)

COL20A1 Antibody in Immunocytochemistry (ICC/IF)

COL20A1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 4

COL20A1 Antibody (PA5-101302) in ICC/IF

Immunocytochemistry analysis of COL20A1 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with COL20A1 polyclonal antibody (Product # PA5-101302) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

COL20A1 Antibody in Immunocytochemistry (ICC/IF)

COL20A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

COL20A1 Antibody in Western Blot (WB)

COL20A1 Antibody in Western Blot (WB)

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COL20A1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of COL20A1 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with COL20A1 polyclonal antibody (Product # PA5-101302) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

COL20A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of COL20A1 in rat lung tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with COL20A1 polyclonal antibody (Product # PA5-101302) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

COL20A1 Antibody in Western Blot (WB) — Western blot analysis of COL20A1 in extracts from various samples. Samples were incubated with COL20A1 polyclonal antibody (Product # PA5-101302). Lane 1: Mouse brain, blocked with antigen-specific peptides; Lane 2: Mouse brain; Lane 3: Raw264.7 cells (serum starvation treatment). Observed bands: 120 kDa.

COL20A1 Antibody in Western Blot (WB) — Western blot analysis of COL20A1 in extracts from various samples. Samples were incubated with COL20A1 polyclonal antibody (Product # PA5-101302). Lane 1: Rat heart, blocked with antigen-specific peptides; Lane 2: Rat heart; Lane 3: Mouse spleen.

Immunogen image

COL20A1 Polyclonal Antibody

Immunogen Image COL20A1 Polyclonal Antibody

产品信息

PA5-101302

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human Collagen XX alpha 1, corresponding to a region within C-terminal amino acids.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody detects endogenous levels of total Collagen XX alpha 1.

靶标信息

Probable collagen protein.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Collagen alpha-1(XX) chain; collagen, type XX, alpha 1; collagen-like protein

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基因别名: 1700051I12Rik; bA261N11.4; COL20A1; KIAA1510; RGD1563901

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UniProt ID: (Human) Q6PI59, (Mouse) Q923P0

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Entrez Gene ID: (Human) 57642, (Rat) 311716, (Mouse) 73368

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molecular_function extracellular matrix structural protein

biological_process