Invitrogen

COPB2 Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他9个COPB2抗体

说明书

实验方案

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说明书

实验方案

技术支持

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COPB2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of COPB2 in U20S cells. Samples were incubated with COPB2 polyclonal antibody (Product # PA5-77106) followed by DAPI (nuclear staining).

COPB2 Antibody in Western Blot (WB) — Western blot analysis of COPB2 in extracts of various cell lines. Samples were incubated with COPB2 polyclonal antibody (Product # PA5-77106).

COPB2 Antibody — Figure 1 The expression levels of COPB2 in human adenocarcinoma cell lines and normal bronchial epithelial cells and the effects of knockdown COPB2 on cell proliferation, apoptosis, and migration in NCI-H1975 human adenocarcinoma cells. ( A-C ) Western blot and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) show the expression level of COPB2 in BEAS-2B normal human bronchial epithelial cells and human lung adenocarcinoma cells, NCI-H1299, A549, SK-MES-1, NCI-H1688, and NCI-H1975. * P <0.05, *** P <0.001 vs. BEAS-2B. ( D-F ) Western blot and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) show the relative expression level of COPB2 in NCI-H1975 cells. ( G ) The apoptosis rate of NCI-H1975 cells was measured by flow cytometry. The percentage of apoptotic cells, determined by propidium iodide (PI) + /Annexin V + , is shown in the right chart. ( H ) Photomicrograph of the transwell assay for NCI-H1975 cells. Magnification x200. The migration rate of NCI-H1975 cells is shown in the right chart. ( I ) Photomicrograph of the transwell assay for NCI-H1975 cells. Magnification x200. The invasion rate of NCI-H1975 cells is shown in the right chart. ( J ) Cell proliferation of NCI-H1975 cells detected by the Cell Counting Kit-8 (CCK-8) assay. *** P <0.001 vs. NC. Control - fluorescence-labeled transfection control siRNA; NC - scrambled negative control siRNA; siCOPB2 - siRNA for knockdown of COPB2. {KD}

产品信息

PA5-77106

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:500-1:2,000	-
免疫组化（石蜡） (IHC (P))	1:50-1:200	-
免疫细胞化学 (ICC/IF)	1:50-1:200	-

产品规格
种属反应	Human, Mouse, Rat
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Recombinant full length Human COPB2. 查看免疫原
偶联物	Unconjugated
形式	Liquid
浓度	1 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS, pH 7.2, with 50% glycerol
内含物	0.02% sodium azide
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2720833

产品详细信息

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

靶标信息

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: beta prime COP; Beta'-coat protein; Beta'-COP; betaprime-COP; coatomer binding complex, beta prime subunit; coatomer protein complex subunit beta 2 (beta prime); coatomer protein complex subunit beta prime; coatomer protein complex, subunit beta 2 (beta prime); Coatomer subunit beta'; p102

基因别名: AI256832; beta'-COP; COPB2

UniProt ID: (Human) P35606, (Rat) O35142, (Mouse) O55029

Entrez Gene ID: (Human) 9276, (Rat) 60384, (Mouse) 50797

分子生物学功能: vesicle coat protein