Invitrogen

COPD Monoclonal Antibody (P1318)

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COPD Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of COPD was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: COPD Monoclonal Antibody (P1318) (Product # MA5-18287) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

COPD Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of COPD was performed in paraffin-embedded rat urinary bladder tissue using COPD Monoclonal Antibody (P1318) (Product # MA5-18287) at a dilution of 1:200.

COPD Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemistry (Frozen) analysis of COPD was performed in frozen-sectioned mouse mouse cerebellum tissue using COPD Monoclonal Antibody (P1318) (Product # MA5-18287) at a dilution of 1:250 (Green). Red: Calbindin, stained by Calbindin antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 10 min.

COPD Antibody in Western Blot (WB) — Western blot analysis of COPD using A) 30 µg Neuro2A whole cell lysate (B) 30 µg GL261 whole cell lysate (C) 30 µg C8D30 whole cell lysate (D) 30 µg NIH-3T3 whole cell lysate (E) 30 µg BCL-1 whole cell lysate (F) 30 µg Raw 264.7 whole cell lysate and G) 30 µg C2Cl2 whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a COPD monoclonal antibody (Product # MA5-18287) at a dilution of 1:1000.

产品信息

MA5-18287

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:500-1:3,000	-
免疫组化（石蜡） (IHC (P))	1:100-1:1,000	-
免疫组化（冰冻） (IHC (F))	1:100-1:1,000	-
免疫细胞化学 (ICC/IF)	1:100-1:1,000	-

产品规格
种属反应	Human, Mouse, Rat
宿主/亚型	Mouse / IgG1
分类	Monoclonal
类型	Antibody
克隆号	P1318
抗原	Recombinant fragment corresponding to a region within amino acids 89 and 511 of COPD
偶联物	Unconjugated
形式	Liquid
浓度	1 mg/mL
纯化类型	Protein G
保存液	PBS
内含物	no preservative
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2539661

产品详细信息

Recommended positive controls: 293T, A431, HeLa, HepG2, Neuro 2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12, PC-12, Rat2.

Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (82%), Chicken (92%), Rhesus Monkey (99%), Bovine (97%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

This gene maps in a region, which include the mixed lineage leukemia and Friend leukemia virus integration 1 genes, where multiple disease-associated chromosome translocations occur. It is an intracellular protein. Archain sequences are well conserved among eukaryotes and this protein may play a fundamental role in eukaryotic cell biology. It has similarities to heat shock proteins and clathrin-associated proteins, and may be involved in vesicle structure or trafficking.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: Archain; archain vesicle transport protein 1; coatomer delta subunit; coatomer delta-COP; coatomer protein complex, subunit delta; coatomer protein delta-COP; Coatomer subunit delta; Delta-coat protein; Delta-COP; pale coat neuro

基因别名: 4632432M07Rik; ARCN1; COPD; nur17

UniProt ID: (Human) P48444, (Mouse) Q5XJY5, (Rat) Q66H80

Entrez Gene ID: (Human) 372, (Mouse) 213827, (Rat) 300674

分子生物学功能: vesicle coat protein