Invitrogen

COX2 Monoclonal Antibody (COX 229)

COX2 Antibody in Immunocytochemistry (ICC/IF)

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COX2 Antibody (35-8200) in ICC/IF

Immunofluorescence analysis of COX-2 was performed using 70% confluent log phase HUVEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with COX2 (COX 229) Mouse Monoclonal Antibody (Product # 35-8200) at 2µg/mLin 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A281... View More

COX2 Antibody in Western Blot, Immunohistochemistry (Paraffin) (WB, IHC (P))

COX2 Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))

COX2 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

COX2 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using COX2 Monoclonal Antibody (COX 229) (Product # 35-8200), shows increased expression of COX2 in HUVEC upon treatment with 1 ng/mL of IL-1ß for 24 hours. {TM}

产品信息

35-8200

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

查看12篇已发表文章 12篇已发表文章

免疫组化 (IHC)

Assay-dependent

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免疫组化（石蜡） (IHC (P))

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免疫细胞化学 (ICC/IF)

2 µg/mL

查看3篇已发表文章 3篇已发表文章

酶联免疫吸附实验 (ELISA)

Assay-dependent

其他PubMed (Misc)

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse, Rat

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

COX 229

抗原

Synthetic peptide from human COX-2

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533224

靶标信息

COX2 converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis, including production of inflammatory prostaglandins. The conversion of arachidonate to prostaglandin H2 is a 2 step reaction: a cyclooxygenase (COX) reaction which converts arachidonate to prostaglandin G2 (PGG2) and a peroxidase reaction in which PGG2 is reduced to prostaglandin H2 (PGH2). It is constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and is up-regulated under pathological conditions, such as in cancer and inflammation (in contrast to the iso-enzyme PTGS1, which is expressed ubiquitously). Up-regulation of COX2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, COX2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis. COX2 is naturally inhibited by calcitriol (the active form of Vitamin D). Glucocorticoids chronically trans-repress PTGS2 gene activity in vivo in part by interfering with transcription initiation and elongation. COX2 is a target of NSAID such as aspirin, which can reduce pain and swelling from inflammation driven by COX2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Genetics and molecular biology

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35-8200 was used in Immunohistochemistry to study COX-2 methylation and expression in N-methyl-N-Nitrosurea-induced intestinal gastric cancer in Sapajus apella animals.

Thu Sep 28 00:00:00 UTC 2023

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Thu May 12 00:00:00 UTC 2022

Interleukin-26 Has Synergistic Catabolic Effects with Palmitate in Human Articular Chondrocytes via the TLR4-ERK1/2-c-Jun Signaling Pathway.

Cells

Chen YT,Wang CC,Cheng CP,Liu FC,Lee CH,Lee HS,Peng YJ

Published figure using COX2 monoclonal antibody (Product # 35-8200) in Western Blot

Tue Sep 21 00:00:00 UTC 2021

Epigenetic reprogramming of epithelial-mesenchymal transition promotes ferroptosis of head and neck cancer.

Redox biology

Lee J,You JH,Kim MS,Roh JL

Published figure using COX2 monoclonal antibody (Product # 35-8200) in Western Blot

Thu Oct 01 00:00:00 UTC 2020

Epigenetic reprogramming of epithelial-mesenchymal transition promotes ferroptosis of head and neck cancer.

Redox biology

Lee J,You JH,Kim MS,Roh JL

Published figure using COX2 monoclonal antibody (Product # 35-8200) in Western Blot

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N-AS-triggered SPMs are direct regulators of microglia in a model of Alzheimer's disease.

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Lee JY,Han SH,Park MH,Song IS,Choi MK,Yu E,Park CM,Kim HJ,Kim SH,Schuchman EH,Jin HK,Bae JS

35-8200 was used in Immunohistochemistry to identify a role of N-AS in the dysfunction of microglia in AD.

Tue May 12 00:00:00 UTC 2020

N-AS-triggered SPMs are direct regulators of microglia in a model of Alzheimer's disease.

Nature communications

Lee JY,Han SH,Park MH,Song IS,Choi MK,Yu E,Park CM,Kim HJ,Kim SH,Schuchman EH,Jin HK,Bae JS

35-8200 was used in Immunohistochemistry to identify a role of N-AS in the dysfunction of microglia in AD.

Tue May 12 00:00:00 UTC 2020

Secreted Frizzled-Related Protein 1 as a Biomarker against Incomplete Age-Related Lobular Involution and Microcalcifications' Development.

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35-8200 was used in Immunohistochemistry to highlight two SFRP1-based predictive models for incomplete lobular involution and the development of microcalcifications and identified two distinct inflammatory profiles associated with age-related lobular involution in parous and nulliparous women.

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Melaleuca styphelioides Sm. Polyphenols Modulate Interferon Gamma/Histamine-Induced Inflammation in Human NCTC 2544 Keratinocytes.

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Albouchi F,Avola R,Dico GML,Calabrese V,Graziano ACE,Abderrabba M,Cardile V

Published figure using COX2 monoclonal antibody (Product # 35-8200) in Western Blot

Tue Oct 02 00:00:00 UTC 2018

Lactobacillus casei Strain Shirota Enhances the In Vitro Antiproliferative Effect of Geniposide in Human Oral Squamous Carcinoma HSC-3 Cells.

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35-8200 was used in Western Blotting to investigate the enhanced anti-proliferative effect of Lactobacillus casei strain Shirota on geniposide actions in human oral squamous carcinoma HSC-3 cells.

Thu May 03 00:00:00 UTC 2018

Evaluation of Antioxidant Intakes in Relation to Inflammatory Markers Expression Within the Normal Breast Tissue of Breast Cancer Patients.

Integrative cancer therapies

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35-8200 was used in Immunohistochemistry to evaluate correlations between antioxidant intakes and protein expression levels of inflammatory markers in normal breast tissue of breast cancer patients.

Fri Dec 01 00:00:00 UTC 2017

Association between local inflammation and breast tissue age-related lobular involution among premenopausal and postmenopausal breast cancer patients.

PloS one

Hanna M,Dumas I,Orain M,Jacob S,Têtu B,Sanschagrin F,Bureau A,Poirier B,Diorio C

35-8200 was used in Immunohistochemistry to investigate whether local inflammation in the breast tissue is associated with age-related lobular involution.

Thu Oct 19 00:00:00 UTC 2017

Increased COX-2 expression in epithelial and stromal cells of high mammographic density tissues and in a xenograft model of mammographic density.

Breast cancer research and treatment

Chew GL,Huo CW,Huang D,Hill P,Cawson J,Frazer H,Hopper JL,Haviv I,Henderson MA,Britt K,Thompson EW

35-8200 was used in immunohistochemistry to examine Cox-2 expression in epithelial and stromal cells in breast cancer samples.

Sat Aug 01 00:00:00 UTC 2015

Amelioration of hypoxia and LPS-induced intestinal epithelial barrier dysfunction by emodin through the suppression of the NF-κB and HIF-1α signaling pathways.

International journal of molecular medicine

Lei Q,Qiang F,Chao D,Di W,Guoqian Z,Bo Y,Lina Y

35-8200 was used in western blot to demonstrate that emodin attenuates LPS- and hypoxia/reoxygenation-induced intestinal epithelial barrier dysfunction by inhibiting the HIF-1alpha and NF-kappaB signaling pathways, which regulate tight junctions.

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Thu May 01 00:00:00 UTC 2014

COX-2 overexpression and -8473 T/C polymorphism in 3' UTR in non-small cell lung cancer.

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Bhat IA,Rasool R,Qasim I,Masoodi KZ,Paul SA,Bhat BA,Ganaie FA,Aziz SA,Shah ZA

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GRSF1 regulates RNA processing in mitochondrial RNA granules.

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Tue Mar 05 00:00:00 UTC 2013

A quantitative proteomic analysis uncovers the relevance of CUL3 in bladder cancer aggressiveness.

PloS one

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35-8200 was used in Western Blotting to perform a proteomic analysis comparing an invasive T24 and an aggressive metastatic derived T24T bladder cancer cell line.

Tue Jul 02 00:00:00 UTC 2013

Roles of E-cadherin and cyclooxygenase enzymes in predicting different survival patterns of optimally cytoreduced serous ovarian cancer patients.

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British journal of cancer

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Tue Feb 01 00:00:00 UTC 2011

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Asthmatics exhibit altered oxylipin profiles compared to healthy individuals after subway air exposure.

PloS one

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Thu Dec 22 00:00:00 UTC 2011

Asthmatics exhibit altered oxylipin profiles compared to healthy individuals after subway air exposure.

PloS one

Lundström SL,Levänen B,Nording M,Klepczynska-Nyström A,Sköld M,Haeggström JZ,Grunewald J,Svartengren M,Hammock BD,Larsson BM,Eklund A,Wheelock ÅM,Wheelock CE

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Thu Dec 22 00:00:00 UTC 2011

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Journal of experimental & clinical cancer research : CR

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35-8200 was used in immunocytochemistry and immunohistochemistry - paraffin section to investigate mechanisms that up-regulate COX-2 with VEGF in non-small cell lung cancer.

Mon Jan 10 00:00:00 UTC 2011

Cyclooxygenase-2 up-regulates vascular endothelial growth factor via a protein kinase C pathway in non-small cell lung cancer.

Journal of experimental & clinical cancer research : CR

Luo H,Chen Z,Jin H,Zhuang M,Wang T,Su C,Lei Y,Zou J,Zhong B

35-8200 was used in immunocytochemistry and immunohistochemistry - paraffin section to investigate mechanisms that up-regulate COX-2 with VEGF in non-small cell lung cancer.

Mon Jan 10 00:00:00 UTC 2011

Dissecting spatio-temporal protein networks driving human heart development and related disorders.

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Lage K,Møllgård K,Greenway S,Wakimoto H,Gorham JM,Workman CT,Bendsen E,Hansen NT,Rigina O,Roque FS,Wiese C,Christoffels VM,Roberts AE,Smoot LB,Pu WT,Donahoe PK,Tommerup N,Brunak S,Seidman CE,Seidman JG,Larsen LA

35-8200 was used in immunohistochemistry - paraffin section to report the first systematic analysis of spatio-temporal protein networks driving the many stages of heart development

Tue Jun 22 00:00:00 UTC 2010

Dissecting spatio-temporal protein networks driving human heart development and related disorders.

Molecular systems biology

35-8200 was used in immunohistochemistry - paraffin section to report the first systematic analysis of spatio-temporal protein networks driving the many stages of heart development

Tue Jun 22 00:00:00 UTC 2010

Expression of cyclooxygenase-2 in choroidal neovascular membranes from age-related macular degeneration patients.

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35-8200 was used in immunohistochemistry - paraffin section to study the expression of cyclooxygenase-2 in human choroidal neovascular membranes

Sun Feb 01 00:00:00 UTC 2009

Prognostic significance of cyclooxygenase-2 overexpression in glottic cancer.

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Sackett MK,Bairati I,Meyer F,Jobin E,Lussier S,Fortin A,Gélinas M,Nabid A,Brochet F,Têtu B

35-8200 was used in immunohistochemistry - paraffin section to test if COX-2 is a prognostic factor in glottic cancer

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The use of a cyclooxygenase-2 inhibitor (Nepafenac) in an ocular and metastatic animal model of uveal melanoma.

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Marshall JC,Fernandes BF,Di Cesare S,Maloney SC,Logan PT,Antecka E,Burnier MN

35-8200 was used in immunohistochemistry - paraffin section to assess the efficiency of Nepafenac, a topically administered COX-2 inhibitor, in a rabbit model of uveal melanoma

Sat Sep 01 00:00:00 UTC 2007

Expression of protease-activated receptor-1 and -2 in orofacial granulomatosis.

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Ketabchi S,Massi D,Ficarra G,Rubino I,Franchi A,Paglierani M,Simoni A,Capodiferro S,Favia G,Maiorano E,Tarantini F,Cirino G,Santucci M

35-8200 was used in immunohistochemistry - paraffin section to determine the expression of PAR-1, PAR-2, MMP-2, MMP-9, COX-1, and COX-2 in patients with orofacial granulomatosis

Sun Jul 01 00:00:00 UTC 2007

Expression of cyclo-oxygenase-2 in macrophages associated with cutaneous melanoma at different stages of progression.

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Bianchini F,Massi D,Marconi C,Franchi A,Baroni G,Santucci M,Mannini A,Mugnai G,Calorini L

35-8200 was used in immunohistochemistry - paraffin section to investigate the role of COX-2 expression in tumor-associated macrophages from melanoma

Fri Jun 01 00:00:00 UTC 2007

Cyclooxygenase-2 (COX-2) overexpression in meningiomas: real time PCR and immunohistochemistry.

Applied immunohistochemistry & molecular morphology : AIMM

Buccoliero AM,Castiglione F,Rossi Degl'Innocenti D,Arganini L,Taddei A,Ammannati F,Mennonna P,Taddei GL

35-8200 was used in immunohistochemistry - paraffin section to study cyclooxygenase-2 expression in meningiomas

Fri Jun 01 00:00:00 UTC 2007

The expression of cyclooxygenase 2 in retinoblastoma: primary enucleated eyes and enucleation after conservative treatment.

American journal of ophthalmology

Souza Filho JP,Martins MC,Correa ZM,Odashiro AN,Antecka E,Coutinho AB,Macedo CR,Vianna RN,Burnier MN

35-8200 was used in immunohistochemistry - paraffin section to examine the relationship between cyclooxygenase-2 expression and histopathologic features of retinoblastoma specimens treated either by primary or secondary enucleation

Sun Oct 01 00:00:00 UTC 2006

Cyclooxygenase-2 and inducible nitric oxide synthase expression in thyroid neoplasms and their clinicopathological correlation.

Journal of Korean medical science

Kim KH,Kim SH,Kim SH,Back JH,Park MJ,Kim JM

Published figure using COX2 monoclonal antibody (Product # 35-8200) in Immunohistochemistry

Fri Dec 01 00:00:00 UTC 2006

Cyclooxygenase-2 and inducible nitric oxide synthase expression in thyroid neoplasms and their clinicopathological correlation.

Journal of Korean medical science

Kim KH,Kim SH,Kim SH,Back JH,Park MJ,Kim JM

Published figure using COX2 monoclonal antibody (Product # 35-8200) in Immunohistochemistry

Fri Dec 01 00:00:00 UTC 2006

Pulmonary cryptosporidiosis: role of COX2 and NF-kB.

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica

Asaad NY,Sadek GS

35-8200 was used in immunohistochemistry - paraffin section to test if Cryptosporidium-induced changes in the lungs are mediated through the activation of COX-2 and NF-kB

Sun Oct 01 00:00:00 UTC 2006

Cyclooxygenase-2 (COX-2) overexpression in childhood brain tumors.

In vivo (Athens, Greece)

Bodey B,Siegel SE,Kaiser HE

35-8200 was used in immunohistochemistry - paraffin section to study COX-2 receptor expression in primary childhood brain tumors

Thu Sep 14 00:00:00 UTC 2006

Differential gene expression in colon cancer of the caecum versus the sigmoid and rectosigmoid.

Gut

Birkenkamp-Demtroder K,Olesen SH,Sørensen FB,Laurberg S,Laiho P,Aaltonen LA,Orntoft TF

Tue Mar 01 00:00:00 UTC 2005

Cyclooxygenase-2 is expressed in neuroblastoma, and nonsteroidal anti-inflammatory drugs induce apoptosis and inhibit tumor growth in vivo.

Cancer research

Johnsen JI,Lindskog M,Ponthan F,Pettersen I,Elfman L,Orrego A,Sveinbjörnsson B,Kogner P

Fri Oct 15 00:00:00 UTC 2004

生物信息学

蛋白别名: COX-2; cyclooxygenase; cyclooxygenase 2; cyclooxygenase 2b; Cyclooxygenase-2; Glucocorticoid-regulated inflammatory cyclooxygenase; Gripghs; Macrophage activation-associated marker protein P71/73; PES-2; PGH synthase 2; PHS II; Prostaglandin G/H synthase 2; Prostaglandin H2 synthase 2; Prostaglandin-endoperoxide synthase 2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase); TIS10 protein

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基因别名: COX-2; COX2; GRIPGHS; hCox-2; PGG/HS; PGHS-2; Pghs-b; Pghs2; PHS-2; PTGS2; TIS10

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UniProt ID: (Human) P35354, (Mouse) Q05769

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Entrez Gene ID: (Human) 5743, (Mouse) 19225

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功能