Invitrogen

CPT1A Polyclonal Antibody

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. 查看细节

查看其他6个CPT1A抗体

说明书

Protocols

技术支持

说明书

Protocols

技术支持

Product Image Gallery

CPT1A Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Carnitine O-palmitoyl transferase 1, liver isoform was performed using 70% confluent log phase MCF7 and MCF 10A cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CPT1A Polyclonal Antibody (Product # PA5-76788) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing mitochondria like localization. Panel e represents MCF 10A cells with less expression of CPT1A. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

CPT1A Antibody in Western Blot (WB) — Western blot was performed using Anti-CPT1A Polyclonal Antibody (Product # PA5-76788) and a 86kDa band corresponding to Carnitine O-palmitoyl transferase 1, liver isoform was observed across cell lines tested. Expression of CPT1A is observed to be high in MCF7 when compared to MDA-MB-231 and MCF 10A as reported in literatures. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), HEK-293 (Lane 2), SK-O-V3 (Lane 3), MCF7 (Lane 4), MDA-MB-231 (Lane 5), MCF 10A (Lane 6), PANC-1 (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:6000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

CPT1A Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of Carnitine O-palmitoyltransferase 1, liver isoform was observed in MCF7 when compared to MDA-MB-231 and MCF 10A using Anti-CPT1A Polyclonal Antibody (Product # PA5-76788) in Western Blot. (DOI:10.1186/s40170-016-0163-7). {RE}

产品细节

Applications

Tested Dilution

Publications

免疫印迹 (WB)

1:500-1:2,000

免疫细胞化学 (ICC/IF)

1:50-1:200

免疫沉淀 (IP)

1:50-1:100

产品规格

种属反应

Human, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant full length Human CPT1A.

偶联物

Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 50% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2720515

产品详细信息

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

靶标信息

The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

引用文献 (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

生物信息学

蛋白别名: Carnitine O-palmitoyltransferase 1, liver isoform; Carnitine O-palmitoyltransferase I, liver isoform; Carnitine palmitoyltransferase 1 alpha, liver isoform; carnitine palmitoyltransferase 1, liver; Carnitine palmitoyltransferase 1A; carnitine palmitoyltransferase 1A (liver); carnitine palmitoyltransferase I, liver; CPT 1; CPT I; CPT1 L; CPT1-L; CPTI-L

基因别名: Cpt-1; CPT-Ia; CPT1; CPT1-L; CPT1A; L-CPT1

UniProt ID: (Human) P50416, (Rat) P32198

Entrez Gene ID: (Human) 1374, (Rat) 25757

分子生物学功能: acyltransferase