Invitrogen

CPT1A Polyclonal Antibody

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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说明书

实验方案

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CPT1A Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Carnitine O-palmitoyl transferase 1, liver isoform was performed using 70% confluent log phase MCF7 and MCF 10A cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CPT1A Polyclonal Antibody (Product # PA5-76788) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing mitochondria like localization. Panel e represents MCF 10A cells with less expression of CPT1A. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

CPT1A Antibody in Western Blot (WB) — Western blot was performed using Anti-CPT1A Polyclonal Antibody (Product # PA5-76788) and a 86kDa band corresponding to Carnitine O-palmitoyl transferase 1, liver isoform was observed across cell lines tested. Expression of CPT1A is observed to be high in MCF7 when compared to MDA-MB-231 and MCF 10A as reported in literatures. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), HEK-293 (Lane 2), SK-O-V3 (Lane 3), MCF7 (Lane 4), MDA-MB-231 (Lane 5), MCF 10A (Lane 6), PANC-1 (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:6000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

CPT1A Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of Carnitine O-palmitoyltransferase 1, liver isoform was observed in MCF7 when compared to MDA-MB-231 and MCF 10A using Anti-CPT1A Polyclonal Antibody (Product # PA5-76788) in Western Blot. (DOI:10.1186/s40170-016-0163-7). {RE}

产品信息

PA5-76788

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:500-1:2,000	-
免疫组化 (IHC)	1:50-1:200	-
免疫细胞化学 (ICC/IF)	1:50-1:200	-

产品规格
种属反应	Human, Rat
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Recombinant full length Human CPT1A.
偶联物	Unconjugated
形式	Liquid
浓度	1 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS with 50% glycerol
内含物	0.02% sodium azide
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2720515

产品详细信息

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

靶标信息

The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: Carnitine O-palmitoyltransferase 1, liver isoform; Carnitine O-palmitoyltransferase I, liver isoform; Carnitine palmitoyltransferase 1 alpha, liver isoform; carnitine palmitoyltransferase 1, liver; Carnitine palmitoyltransferase 1A; carnitine palmitoyltransferase 1A (liver); carnitine palmitoyltransferase I, liver; CPT 1; CPT I; CPT1 L; CPT1-L; CPTI-L

基因别名: Cpt-1; CPT-Ia; CPT1; CPT1-L; CPT1A; L-CPT1

UniProt ID: (Human) P50416, (Rat) P32198

Entrez Gene ID: (Human) 1374, (Rat) 25757

分子生物学功能: acyltransferase