Proteintech

CTBP1 Polyclonal Antibody

6 篇参考文献

查看其他23个CtBP1抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (13)

CTBP1 Antibody in Immunocytochemistry (ICC/IF)

CTBP1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 13

CTBP1 Antibody (10972-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CTBP1 antibody (Product # 10972-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

CTBP1 Antibody in Immunocytochemistry (ICC/IF)

CTBP1 Antibody in Immunocytochemistry (ICC/IF)

CTBP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CTBP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CTBP1 Antibody in Western Blot (WB)

CTBP1 Antibody in Western Blot (WB)

CTBP1 Antibody in Western Blot (WB)

CTBP1 Antibody in Western Blot (WB)

CTBP1 Antibody in Western Blot (WB)

CTBP1 Antibody in Western Blot (WB)

CTBP1 Antibody in Western Blot (WB)

CTBP1 Antibody in Western Blot (WB)

CTBP1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

CTBP1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CTBP1 antibody (Product # 10972-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

CTBP1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using 10972-1-AP (CTBP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

CTBP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue slide using (Product # 10972-1-AP) CTBP1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CTBP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human prostate cancer using 10972-1-AP (CTBP1 antibody) at dilution of 1:50 (under 40x lens).

CTBP1 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with (Product # 10972-1-AP) CTBP1 antibody at dilution of 1:4,000 incubated at room temperature for 1.5 hours.

CTBP1 Antibody in Western Blot (WB) — Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10972-1-AP (CTBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

CTBP1 Antibody in Western Blot (WB) — Mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10972-1-AP (CTBP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

CTBP1 Antibody in Western Blot (WB) — Mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10972-1-AP (CTBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

CTBP1 Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 10972-1-AP (CTBP1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

CTBP1 Antibody in Western Blot (WB) — Jurkat cells were subjected to SDS PAGE followed by western blot with 10972-1-AP (CTBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

CTBP1 Antibody in Western Blot (WB) — HL-60 cells were subjected to SDS PAGE followed by western blot with 10972-1-AP (CTBP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

CTBP1 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 10972-1-AP (CTBP1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

CTBP1 Antibody in Immunoprecipitation (IP) — IP result of anti-CTBP1 (IP:10972-1-AP, 3ug; Detection:10972-1-AP 1:200) with mouse brain tissue lysate 4000ug.

Immunogen image

CTBP1 Polyclonal Antibody

Immunogen Image CTBP1 Polyclonal Antibody

产品信息

10972-1-AP

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

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免疫组化（石蜡） (IHC (P))

1:20-1:200

-

免疫细胞化学 (ICC/IF)

1:50-1:500

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

0.5-4.0 µg

-

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

CTBP1 Fusion Protein Ag1425 (1-440 aa encoded by BC011655)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.17 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice (domestic); Dry ice (international)

产品详细信息

Immunogen sequence: MGSSHLLNK GLPLGVRPPI MNGPLHPRPL VALLDGRDCT VEMPILKDVA TVAFCDAQST QEIHEKVLNE AVGALMYHTI TLTREDLEKF KALRIIVRIG SGFDNIDIKS AGDLGIAVCN VPAASVEETA DSTLCHILNL YRRATWLHQA LREGTRVQSV EQIREVASGA ARIRGETLGI IGLGRVGQAV ALRAKAFGFN VLFYDPYLSD GVERALGLQR VSTLQDLLFH SDCVTLHCGL NEHNHHLIND FTVKQMRQGA FLVNTARGGL VDEKALAQAL KEGRIRGAAL DVHESEPFSF SQGPLKDAPN LICTPHAAWY SEQASIEMRE EAAREIRRAI TGRIPDSLKN CVNKDHLTAA THWASMDPAV VHPELNGAAY RYPPGVVGVA PTGIPAAVEG IVPSAMSLSH GLPPVAHPPH APSPGQTVKP EADRDHASDQ L (1-440 aa encoded by BC011655)

靶标信息

This gene encodes a protein that binds to the C-terminus of adenovirus E1A proteins. This phosphoprotein is a transcriptional repressor and may play a role during cellular proliferation. This protein and the product of a second closely related gene, CTBP2, can dimerize. Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. Alternative splicing of transcripts from this gene results in multiple transcript variants.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: brefeldin A-ribosylated substrate; C-terminal-binding protein 1; CtBP1; MGC104684

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基因别名: BARS; CTBP; CTBP1; CtBP1-L; CtBP1-S; CtBP3/BARS; D4S115h; D5H4S115; D5H4S115E

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UniProt ID: (Human) Q13363, (Mouse) O88712

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Entrez Gene ID: (Human) 1487, (Mouse) 13016

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RNA polymerase II transcription corepressor activity transcription factor activity, sequence-specific DNA binding protein binding protein C-terminus binding transcription factor binding oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor protein domain specific binding protein homodimerization activity NAD binding repressing transcription factor binding C-3 sterol dehydrogenase (C-4 sterol decarboxylase) activity aldo-keto reductase (NADP) activity isocitrate dehydrogenase activity mevaldate reductase activity gluconate dehydrogenase activity steroid dehydrogenase activity oxidoreductase activity epoxide dehydrogenase activity 5-exo-hydroxycamphor dehydrogenase activity 2-hydroxytetrahydrofuran dehydrogenase activity acetoin dehydrogenase activity phenylcoumaran benzylic ether reductase activity D-xylose:NADP reductase activity L-arabinose:NADP reductase activity D-arabinitol dehydrogenase, D-ribulose forming (NADP+) activity steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor steroid dehydrogenase activity, acting on the CH-CH group of donors (R)-(-)-1,2,3,4-tetrahydronaphthol dehydrogenase activity 3-hydroxymenthone dehydrogenase activity very long-chain-3-hydroxyacyl-CoA dehydrogenase activity dihydrotestosterone 17-beta-dehydrogenase activity (R)-2-hydroxyisocaproate dehydrogenase activity L-arabinose 1-dehydrogenase (NADP+) activity L-xylulose reductase (NAD+) activity 3-ketoglucose-reductase activity (R)-2-hydroxyglutarate dehydrogenase activity D-arabinitol dehydrogenase, D-xylulose forming (NADP+) activity transcription cofactor

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated protein phosphorylation negative regulation of cell proliferation viral genome replication positive regulation of histone deacetylation negative regulation of histone acetylation negative regulation of transcription, DNA-templated white fat cell differentiation regulation of cell cycle oxidation-reduction process negative regulation of histone H4 acetylation negative regulation of transcription from RNA polymerase II promoter by histone modification regulation of transcription, DNA-templated transcription from RNA polymerase II promoter metabolic process cell differentiation