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Invitrogen
CX3CR1 Monoclonal Antibody (2A9-1), Super Bright™ 702, eBioscience™
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CX3CR1 Antibody (67-6099-42) in Flow
Normal human peripheral blood cells were stained with CD56 Monoclonal Antibody, eFluor 450 (Product # 48-0566-42) and Rat IgG2b kappa Isotype Control, Super Bright 702 (Product # 67-4031-82) (left) or CX3CR1 Monoclonal Antibody, Super Bright 702 (right). Cells in the lymphocyte gate were used for analysis.
产品信息
67-6099-42
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
C. elegans
宿主/亚型
Rat
/ IgG2b, kappa
分类
Monoclonal
类型
Antibody
克隆号
2A9-1
偶联物
激发/发射光谱
413/702 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with BSA
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: This 2A9-1 monoclonal antibody reacts with human CX3CR1, which is the receptor for fractalkine, a transmembrane chemokine of the CX3C family. CX3CR1 is a seven transmembrane G protein-coupled receptor expressed on CD16+ NK cells, T cells (e.g. CD8+, CD4+, and gamma/delta), and monocytes. In non-immune cells, CX3CR1 has been found on osteoclast precursors and microglia. Little to no CX3CR1 surface expression can be detected on B cells and granulocytes. Together, fractalkine and its receptor mediate cell-cell adhesion and chemotaxis of NK cells, T cells, and monocytes. The expression of CX3CR1 has also been correlated with high levels of intracellular perforin and granzyme B.
Applications Reported: This 2A9-1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 2A9-1 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Super Bright 702 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 702 nm. We recommend using a 710/50 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 405 nm; Emission: 702 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
靶标信息
CX3CR1 is one of the chemokine receptors that are required as coreceptors for HIV infection. The genes encoding human, mouse, and rat CX3CR1 were cloned and designated V28 and CMKBRL1, CX3CR1, and RBS11, respectively. The encoded seven transmembrane protein was recently identified as the receptor for a novel transmembrane molecule, fractalkine, and renamed CX3CR1. Recently, CX3CR1 was found to serve as a coreceptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by fractokine. CX3CR1 mediates leukocyte migration an d adhesion. CX3CR1 is expressed in a variety of human tissues and cell lines.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: Beta chemokine receptor-like 1; C-X3-C CKR-1; chemokine (C-C) receptor-like 1; chemokine (C-X3-C motif) receptor 1; chemokine (C-X3-C) receptor 1; CMK-BRL-1; CMK-BRL1; Cx3c; CX3C chemokine receptor 1; Fractalkine receptor; G protein-coupled receptor 13; G-protein coupled receptor 13; Scyd1; V28
基因别名: CCRL1; CMKBRL1; CMKDR1; CX3CR1; GPR13; GPRV28; V28
UniProt ID: (Human) P49238
Entrez Gene ID: (Human) 1524
positive regulation of neuroblast proliferation
microglial cell activation involved in immune response
negative regulation of chronic inflammatory response to non-antigenic stimulus
chemotaxis
cellular defense response
cell adhesion
G-protein coupled receptor signaling pathway
memory
response to wounding
viral process
negative regulation of angiogenesis
cerebral cortex cell migration
negative regulation of cell migration
positive regulation of angiogenesis
macrophage chemotaxis
chemokine-mediated signaling pathway
cellular response to lipopolysaccharide
cellular response to transforming growth factor beta stimulus
negative regulation of long-term synaptic potentiation
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
