Invitrogen

CXCL1 Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

3个已发表图片

3篇引用文献

查看其他18个CXCL1 (GRO alpha)抗体

说明书

Protocols

技术支持

说明书

Protocols

技术支持

Product Image Gallery

CXCL1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Growth-regulated alpha protein was performed using 70% confluent log phase A-375 cells treated with 10 ng/mL TNF alpha for 24 hours and untreated A-375 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CXCL1 Polyclonal Antibody (Product # PA5-86508) at 1:100 dilution in 0.1% BSA, incubated at 4°C overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green) in A-375 treated cells. Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization in TNF alpha treated A-375 cells. Panel e represents untreated A-375 cells that show no expression of CXCL1. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

CXCL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of CXCL1 in paraffin-embedded human tonsil carcinoma tissue. Sample was incubated with polyclonal CXCL1 antibody (Product # PA5-86508) using a 1:50 dilution.

CXCL1 Antibody in Western Blot (WB) — Western blot was performed using Anti-CXCL1 Polyclonal Antibody (Product # PA5-86508) and a 9 kDa band corresponding to soluble cleaved form of CXCL1 was observed across cell lines tested. Conditioned media (20 µg lysate) of Caki-1, positive model (Lane 1) and HT-29, negative model (Lane 2) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Atto Ultimate Sensitivity Substrate (Product # A38556).

CXCL1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A-375 cells were transfected with CXCL1 siRNA followed by TNF alpha treatment and decrease in signal intensity was observed in ICC application using Anti-CXCL1 Polyclonal Antibody (Product # PA5-86508). {KD}

CXCL1 Antibody in Immunohistochemistry (IHC) — Figure 6 The keratinocyte-target lipid-polymer nanohybrids suppress IMQ-induced cytokine and chemokine expression, as well as neutrophil infiltration in psoriasis-like skin. IHC analysis represents (A) IL-17, (B) CXCL1, (C) CXCL2, (D) Ki67 + , (E) Ly6G, and (F) MPO in psoriasis-like skin. Scale bars, 100 um.

产品信息

PA5-86508

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:500-1:1,000	View 1 publication 1 publication
免疫组化 (IHC)	-	View 1 publication 1 publication
免疫组化（石蜡） (IHC (P))	1:50-1:200	View 1 publication 1 publication
免疫细胞化学 (ICC/IF)	1:100	-

产品规格
种属反应	Human, Mouse, Rat
已发表种属	Mouse, Rat
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Synthetic peptide, corresponding to amino acids 41-90 of Human GROalpha.
偶联物	Unconjugated
形式	Liquid
浓度	1 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS, pH 7.2, with 50% glycerol
内含物	0.02% sodium azide
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2803283

靶标信息

CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

CXCL1 Regulated by miR-302e Is Involved in Cell Viability and Motility of Colorectal Cancer via Inhibiting JAK-STAT Signaling Pathway.

Frontiers in oncology

Chen B,Song L,Nie X,Lin F,Yu Z,Kong W,Qi X,Wang W

PA5-86508 was used in Immunohistochemistry (Paraffin) to make a systemic description for the CXCL1-dependent regulatory mechanism in colorectal cancer.

Sat Apr 23 00:00:00 EDT 2022

CXCL1 Regulated by miR-302e Is Involved in Cell Viability and Motility of Colorectal Cancer via Inhibiting JAK-STAT Signaling Pathway.

Frontiers in oncology

Chen B,Song L,Nie X,Lin F,Yu Z,Kong W,Qi X,Wang W

PA5-86508 was used in Immunohistochemistry (Paraffin) to make a systemic description for the CXCL1-dependent regulatory mechanism in colorectal cancer.

Sat Apr 23 00:00:00 EDT 2022

Monovalent antibody-conjugated lipid-polymer nanohybrids for active targeting to desmoglein 3 of keratinocytes to attenuate psoriasiform inflammation.

Theranostics

Lin ZC,Hwang TL,Huang TH,Tahara K,Trousil J,Fang JY

PA5-86508 was used in Immunohistochemistry to develop actively targeted nanocarriers loaded with the phosphodiesterase 4 inhibitor AN2728.

Mon Aug 16 00:00:00 EDT 2021

Monovalent antibody-conjugated lipid-polymer nanohybrids for active targeting to desmoglein 3 of keratinocytes to attenuate psoriasiform inflammation.

Theranostics

Lin ZC,Hwang TL,Huang TH,Tahara K,Trousil J,Fang JY

PA5-86508 was used in Immunohistochemistry to develop actively targeted nanocarriers loaded with the phosphodiesterase 4 inhibitor AN2728.

Mon Aug 16 00:00:00 EDT 2021

Involvement of CXCL1/CXCR2 During Microglia Activation Following Inflammation-Sensitized Hypoxic-Ischemic Brain Injury in Neonatal Rats.

Frontiers in neurology

Serdar M,Kempe K,Herrmann R,Picard D,Remke M,Herz J,Bendix I,Felderhoff-Müser U,Sabir H

PA5-86508 was used in Western Blot to indicate that the CXCL1/CXCR2 pathway may be involved during pro-inflammatory microglia activation following inflammation-sensitized hypoxic-ischemic brain injury in neonatal rats.

Sat Oct 31 00:00:00 EDT 2020

Involvement of CXCL1/CXCR2 During Microglia Activation Following Inflammation-Sensitized Hypoxic-Ischemic Brain Injury in Neonatal Rats.

Frontiers in neurology

Serdar M,Kempe K,Herrmann R,Picard D,Remke M,Herz J,Bendix I,Felderhoff-Müser U,Sabir H

Sat Oct 31 00:00:00 EDT 2020

生物信息学

蛋白别名: alpha-chemokine; C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 1; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha); CINC-1; CXC; CXC motif chemokine; CXCL; CXCL-1; Cytokine-induced neutrophil chemoattractant 1; fibroblast secretory protein; gro; GRO a; GRO α; GRO-alpha(1-73); GRO-alpha(4-73); GRO-alpha(5-73); GRO-alpha(6-73); GRO1 oncogene; GRO1 oncogene (melanoma growth stimulating activity, alpha); GRO1 oncogene (melanoma growth-stimulating activity); GROalpha; Growth-regulated alpha protein; GROα; Hematopoietic synergistic factor; HSF; KC(5-72); KC-T; KC/GR)-alpha; KC/GRO-alpha; melanoma growth stimulating activity, alpha; Melanoma growth stimulatory activity; melanoma growth stimulatory activity alpha; MGSA; MGSA alpha; NAP-3; NAP3; Neutrophil-activating protein 3; Platelet-derived growth factor-inducible protein KC; Secretory protein N51

基因别名: CINC-1; Cinc1; CXCL1; FSP; GRO; GRO1; GROA; KC; MGSA; MGSA-a; N51; NAP-3; SCYB1

UniProt ID: (Human) P09341, (Rat) P14095, (Mouse) P12850

Entrez Gene ID: (Human) 2919, (Rat) 81503, (Mouse) 14825

分子生物学功能: chemokine

功能

参与通路

receptor binding chemokine activity enzyme activator activity growth factor activity CXCR chemokine receptor binding cytokine activity

CXCL1 Polyclonal Antibody

产品信息

PA5-86508

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

靶标信息

生物信息学

性能保障

随时为您服务

Brands

Shopping Tools

eSolutions

Support

我们的公司

Your items have has been added!