Invitrogen

CYP1A2 Monoclonal Antibody (3B8C1)

Name: CYP1A2 Monoclonal Antibody (3B8C1)
Brand: Invitrogen
SKU: MA3-037

查看其他11个CYP1A2抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (3)

CYP1A2 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 3

CYP1A2 Antibody (MA3-037) in ICC/IF

Immunofluorescent analysis of Cytochrome P450 1A2 (green) showing staining in the cytoplasm and membrane of COS-7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 1A2 monoclonal antibody (Product # MA3-037) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS ... View More

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产品信息

MA3-037

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:250-1:500

免疫组化（石蜡） (IHC (P))

1-5 µg/mL

免疫细胞化学 (ICC/IF)

1:20-1:200

产品规格

种属反应

Human, Non-human primate, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

3B8C1

抗原

Rat cytochrome P450 protein.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

ascites

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_1073971

产品详细信息

MA3-037 detects Cytochrome P450 1A2 from human and non-human primate samples.

MA3-037 has been successfully used in Western blot, immunocytochemistry and immunofluorescence applications. By Western blot, this antibody detects a ~58 kDa band representing Cytochrome P450 1A2.

The MA3-037 immunogen is rat cytochrome P450 protein.

靶标信息

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. Other xenobiotic substrates for this enzyme include caffeine, aflatoxin B1, and acetaminophen. The transcript from this gene contains four Alu sequences flanked by direct repeats in the 3' untranslated region.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: aryl hydrocarbon hydroxylase; Cholesterol 25-hydroxylase; CYP; CYPIA2; Cytochrome P(3)450; Cytochrome P-448; Cytochrome P-450d; Cytochrome P450 1A2; Cytochrome P450 4; Cytochrome P450 subfamily I (aromatic compound-inducible) member A2 (Q42 form d); cytochrome P450, 1a2; cytochrome P450, family 1, subfamily A, polypeptide 2; cytochrome P450, subfamily I (aromatic compound-inducible), member A2 (Q42, form d); cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 2; Cytochrome P450-D; Cytochrome P450-P3; dioxin-inducible P3-450; flavoprotein-linked monooxygenase; Hydroperoxy icosatetraenoate dehydratase; microsomal monooxygenase; P(3)450; P-448; P450; P450 4; P450 form 4; P450-D; P450-P3; xenobiotic monooxygenase

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基因别名: CP12; Cyp1a-2; CYP1A2; CYPD45; P-450d; P3-450; P450(PA); RATCYPD45

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UniProt ID: (Human) P05177, (Rat) P04799

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Entrez Gene ID: (Human) 1544, (Rat) 24297

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