Invitrogen

CYP3A4/CYP3A5 Monoclonal Antibody (F24 P2 B10)

Name: CYP3A4\/CYP3A5 Monoclonal Antibody (F24 P2 B10)
Brand: Invitrogen
SKU: MA3-032

2 篇参考文献

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (2)

CYP3A4/CYP3A5 Antibody in Immunocytochemistry (ICC/IF)

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CYP3A4/CYP3A5 Antibody (MA3-032) in ICC/IF

Immunofluorescence analysis of Cytochrome P450-3A4-3A5 was done on 70% confluent log phase HepG2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cytochrome P450 3A4 + 3A5 (F24 P2 B10) Mouse Monoclonal Antibody (Product # MA3-032) at 1:250 dilution in ... View More

CYP3A4/CYP3A5 Antibody in Western Blot (WB)

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Immunogen image

Immunogen Image CYP3A4/CYP3A5 Monoclonal Antibody (F24 P2 B10)

产品信息

MA3-032

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

1:250

产品规格

种属反应

Bovine, Human

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

F24 P2 B10

抗原

Synthetic peptide corresponding to residues E S R D G T V S G A, conjugated to ovalbumin.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

ascites

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2261518

产品详细信息

MA3-032 detects Cytochrome P450 3A4/3A5 from human cell line HepG2, and from bovine liver and pancreas samples.

MA3-032 has been successfully used in Western blot applications. By Western blot, this antibody detects a ~54 kDa band representing Cytochrome P450 3A4/3A5.

The MA3-032 immunogen is a synthetic peptide corresponding to residues E S R D G T V S G A, conjugated to ovalbumin.

靶标信息

CYP3A5 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. The enzyme metabolizes drugs such as nifedipine and cyclosporine as well as the steroid hormones testosterone, progesterone and androstenedione.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: 1,4-cineole 2-exo-monooxygenase; 1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; Albendazole monooxygenase (sulfoxide-forming); Albendazole sulfoxidase; aryl hydrocarbon hydroxylase; Cholesterol 25-hydroxylase; CYPIIIA28; CYPIIIA3; CYPIIIA4; CYPIIIA5; cytochrome P450 3A28; Cytochrome P450 3A3; Cytochrome P450 3A4; Cytochrome P450 3A5; Cytochrome P450 HLp; cytochrome P450 HLp2; Cytochrome P450 NF-25; cytochrome P450, family 3, subfamily A, polypeptide 4; cytochrome P450, family 3, subfamily A, polypeptide 5; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 5; Cytochrome P450-PCN1; Cytochrome P450-PCN3; flavoprotein-linked monooxygenase; glucocorticoid-inducible P450; HLp2; microsomal monooxygenase; Nifedipine oxidase; P450-III, steroid inducible; P450-PCN3; Quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase; xenobiotic monooxygenase

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基因别名: CP33; CP34; CP35; CYP3A; CYP3A28; CYP3A3; CYP3A4; CYP3A5; CYPIIIA3; CYPIIIA4; CYPIIIA5; HLP; NF-25; P450C3; P450PCN1; P450PCN3; PCN3

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UniProt ID: (Human) P08684, (Human) P20815