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CaMKIV Antibody (PA5-81302) in ICC/IF
Immunofluorescence staining of Camk4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with CaMKIV Polyclonal Antibody (Product # PA5-81302, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.
Product Details
PA5-81302
Applications
Tested Dilution
Publications
产品规格
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human CaM Kinase 4/CaMKIV
Conjugate
Unconjugated
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A, Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
RRID
AB_2788519
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human CAMKIV.
Target Information
CAMK4 (CaMKIV) is a serine/threonine protein kinase that phosphoryates CREB, oncoprotein 18, and SRF. It has limited tissue distribution, and has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. The nuclear localization of this protein is consistent with its role in mediating calcium-dependent gene expression. CaMKIV is particularly abundant in testis, T-cells, and neurons but is also found in other tissues to varying degrees. In neurons, CaMKIV is thought to play an important role in synaptic plasticity via its gene regulatory effects. In T-cells, this protein plays an important role in calcium signaling which could affect the transcription regulatory protein, nuclear factor of activated T-cells (NFAT). CaMKIV is encoded, along with calspermin, by the CaMKIV gene. It has been found that, in testes, CaMKIV is expressed in germ cells and found to be associated with chromatin. The association of CaMKIV with chromatin suggests a potential role in chromatin remodeling during nuclear condensation in spermatids.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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Bioinformatics
Protein Aliases: brain Ca(2+)-calmodulin-dependent protein kinase type IV; brain Ca++-calmodulin-dependent protein kinase type IV; Calcium/calmoduli; calcium/calmodulin-dependent protein kinase IV; Calcium/calmodulin-dependent protein kinase type IV; calcium/calmodulin-dependent protein kinase type IV catalytic chain; CAM kinase IV; CAM kinase- GR; CaM kinase-GR; CaMK IV; EC 2.7.11.17; KCC4; kinase CaMK4; OTTHUMP00000222818
Gene Aliases: CAMK; CaMK IV; CAMK-GR; CAMK4; CAMKIV
UniProt ID: (Human) Q16566
Entrez Gene ID: (Human) 814
Molecular Function:
non-receptor serine/threonine protein kinase
adaptive immune response
myeloid dendritic cell cytokine production
protein phosphorylation
inflammatory response
signal transduction
long-term memory
peptidyl-serine phosphorylation
regulation of T cell differentiation in thymus
intracellular signal transduction
myeloid dendritic cell differentiation
regulation of osteoclast differentiation
positive regulation of transcription, DNA-templated
protein autophosphorylation
Performance Guarantee
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