Invitrogen

Calbindin D28K Polyclonal Antibody

高级验证

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

4个已发表图片

6 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

Calbindin D28K Antibody in Western Blot (WB)

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Calbindin D28K Antibody (PA1-931) in WB

Western blot analysis was performed using whole cell extracts (30 µg) of Mouse Brain (Lane 1), Rat Brain (Lane 2), IMR-32 (Lane 3), SH-SY5Y (Lane 4) and U-2 OS (Lane 5).The blots were probed with Anti-Calbindin D-28K Rabbit Polyclonal Antibody (Product # PA1-931, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4µg/mL 1:2500 dilution). A ~28 kDa band corresponding to Calbindin D-28K was observed across tissues and cell lines tested. Known... View More

Calbindin D28K Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Calbindin D28K Antibody in Immunocytochemistry (ICC/IF)

Calbindin D28K Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA1-931

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2 µg/mL

免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1 µg/mL

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免疫细胞化学 (ICC/IF)

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酶联免疫吸附实验 (ELISA)

Assay-dependent

免疫沉淀 (IP)

5 µg/mL

中和实验 (Neu)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

28 kDa calbindin-D protein purified from rat kidney.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 15mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2068509

产品详细信息

PA1-931 detects calbindin D-28k protein in rat samples.

PA1-931 has successfully been used in Western blot , ELISA, immunoprecipitation and immunohistochemical procedures. By Western blot, this antibody detects a 28 kDa protein representing calbindin D from rat cerebellum and rat kidney extract. Immunohistochemical staining on paraffin sections demonstrates that calbindin D is localized to the cytoplasm in epithelial cells of distal tubules in human kidney cortex after staining with PA1-931.

The PA1-931 immunizing protein corresponds to the purified 28 kDa calbindin-D protein from rat kidney.

Reconstitute in 100 µL PBS to create a stock of 1 mg/mL.

靶标信息

Calbindin-D-28K (also termed vitamin D-dependent calcium-binding protein, or cholecalcin), is a highly conserved 28kDa calcium binding protein, with broad tissue distribution. It belongs, together with calmodulin, S-100, parvalbumin, troponin C and other proteins, to a family of low molecular weight calcium-binding proteins (CaBPs). These CaBPs have homologous primary structures, which contain conserved polypeptide folds of the EF-hand type for Ca2+ binding. Calbindin-D-28K is found predominantly in subpopulations of central and peripheral nervous system neurons, and in certain epithelial nervous system neurons, and in certain epithelial cells involved in Ca2+ transport such as distal tubular cells and cortical collecting tubules of the kidney, and in enteric neuroendocrine cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Lycii radicis cortex inhibits glucocorticoid‑induced bone loss by downregulating Runx2 and BMP‑2 expression.

International journal of molecular medicine

Lee B,Hong S,Kim M,Kim EY,Park HJ,Jung HS,Kim JH,Sohn Y

PA1-931 was used in Immunohistochemistry (Paraffin) to to suggest that the use of LRC alleviates GC‚induced secondary osteoporosis.

Sun Aug 01 00:00:00 EDT 2021

Lycii radicis cortex inhibits glucocorticoid‑induced bone loss by downregulating Runx2 and BMP‑2 expression.

International journal of molecular medicine

Lee B,Hong S,Kim M,Kim EY,Park HJ,Jung HS,Kim JH,Sohn Y

PA1-931 was used in Immunohistochemistry (Paraffin) to to suggest that the use of LRC alleviates GC‚induced secondary osteoporosis.

Sun Aug 01 00:00:00 EDT 2021

Conditional Deletion of Activating Rearranged During Transfection Receptor Tyrosine Kinase Leads to Impairment of Photoreceptor Ribbon Synapses and Disrupted Visual Function in Mice.

Frontiers in neuroscience

Peng WH,Liao ML,Huang WC,Liu PK,Levi SR,Tseng YJ,Lee CY,Yeh LK,Chen KJ,Chien CL,Wang NK

Published figure using Calbindin D28K polyclonal antibody (Product # PA1-931) in Immunocytochemistry

Sat May 13 00:00:00 EDT 2023

Conditional Deletion of Activating Rearranged During Transfection Receptor Tyrosine Kinase Leads to Impairment of Photoreceptor Ribbon Synapses and Disrupted Visual Function in Mice.

Frontiers in neuroscience

Peng WH,Liao ML,Huang WC,Liu PK,Levi SR,Tseng YJ,Lee CY,Yeh LK,Chen KJ,Chien CL,Wang NK

Published figure using Calbindin D28K polyclonal antibody (Product # PA1-931) in Immunocytochemistry

Sat May 13 00:00:00 EDT 2023

Targeting inhibitory cerebellar circuitry to alleviate behavioral deficits in a mouse model for studying idiopathic autism.

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology

Chao OY,Marron Fernandez de Velasco E,Pathak SS,Maitra S,Zhang H,Duvick L,Wickman K,Orr HT,Hirai H,Yang YM

PA1-931 was used in Immunohistochemistry to highlight complex mechanisms converging onto the cerebellar dysfunction in the phenotypic model and provide effective strategies for potential therapies of ASD.

Mon Jun 01 00:00:00 EDT 2020

Anti-Yo antibody uptake and interaction with its intracellular target antigen causes Purkinje cell death in rat cerebellar slice cultures: a possible mechanism for paraneoplastic cerebellar degeneration in humans with gynecological or breast cancers.

PloS one

Greenlee JE,Clawson SA,Hill KE,Wood B,Clardy SL,Tsunoda I,Carlson NG

PA1-931 was used in blocking or activating experiment to investigate anit-Yo antibodies in Purkinje cell death

Mon Jan 18 00:00:00 EST 2016

PloS one

Greenlee JE,Clawson SA,Hill KE,Wood B,Clardy SL,Tsunoda I,Carlson NG

PA1-931 was used in blocking or activating experiment to investigate anit-Yo antibodies in Purkinje cell death

Mon Jan 18 00:00:00 EST 2016

生物信息学

蛋白别名: Calbindin; calbindin 1, (28kD); calbindin 1, 28 kD; calbindin 1, 28kDa; Calbindin D28; calbindin-28K; calbindin-D (28k); CalbindinD28K; cerebellar Ca-binding protein spot 35 protein; cerebellar Ca-binding protein, spot 35 protein; D-28K; PCD-29; RTVL-H protein; Spot 35 protein; Vitamin D-dependent calcium-binding protein, avian-type

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基因别名: Brain-2; CAB27; CaBP28K; CALB; Calb-1; CALB1; CB; D-28K

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UniProt ID: (Human) P05937, (Rat) P07171, (Mouse) P12658

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Entrez Gene ID: (Human) 793, (Rat) 83839, (Mouse) 12307

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功能

vitamin D binding calcium ion binding protein binding zinc ion binding metal ion binding calmodulin-related

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