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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Calmodulin Antibodies

          Invitrogen

          Calmodulin Monoclonal Antibody (6D4)

          4个已发表图片
          10 篇参考文献
          查看其他13个Calmodulin抗体
          说明书
          实验方案
          问题与答案
          技术支持
          说明书
          实验方案
          问题与答案
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          Cite Calmodulin Monoclonal Antibody (6D4)

          • 抗体检测数据 (2)
          • 已发表图片 (4)
          Calmodulin Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Calmodulin Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          图: 1 / 6

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          Calmodulin Antibody (MA3-918) in ICC/IF

          Immunofluorescence analysis of Calmodulin was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Calmodulin (6D4) Mouse Monoclonal Antibody (Product # MA3-918) at 1:250 dilution in 0.1% BSA and incubated for 3 hours a... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
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          查看产品

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          Calmodulin Antibody in Immunocytochemistry (ICC/IF)
          Calmodulin Antibody in Western Blot (WB)
          Calmodulin Antibody in Immunohistochemistry (IHC)
          Calmodulin Antibody in Immunohistochemistry (IHC)
          Calmodulin Antibody in Western Blot (WB)
          Calmodulin Antibody in Western Blot (WB)

          产品信息

          MA3-918

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1:2,000
          查看5篇已发表文章 5篇已发表文章

          免疫组化 (IHC)

          -
          查看1篇已发表文章 1篇已发表文章

          免疫组织化学(多聚甲醛固定) (IHC (PFA))

          -
          查看1篇已发表文章 1篇已发表文章

          免疫组化(冰冻) (IHC (F))

          -
          查看1篇已发表文章 1篇已发表文章

          免疫细胞化学 (ICC/IF)

          1:250
          查看2篇已发表文章 2篇已发表文章

          酶联免疫吸附实验 (ELISA)

          Assay-dependent
          -
          产品规格

          种属反应

          Bacteria, Bovine, Chicken, Human, Rat

          已发表种属

          Algae, Cnidarian, Coral, Fruit fly, Human, Mouse, Rat

          宿主/亚型

          Mouse / IgG1

          分类

          Monoclonal

          类型

          Antibody

          克隆号

          6D4

          抗原

          Calmodulin purified from Dictyostelium discoideum.
          查看免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          1 mg/mL

          纯化类型

          purified

          保存液

          PBS, pH 7.4, with 1mg/mL BSA

          内含物

          0.05% sodium azide

          保存条件

          -20°C, Avoid Freeze/Thaw Cycles

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_325501

          产品详细信息

          MA3-918 detects calmodulin from bovine, chicken, Chlamydomonas, Dictyostelium, and rat samples. This antibody does not detect parvalbumin, tropinin, S-100, or myosin light chain kinase (MLCK).

          MA3-918 has been successfully used in Western blot, immunocytochemistry and ELISA procedures. By Western blot, this antibody detects a 17 kDa protein representing calmodulin from Dictyostelium cell lysate. Addition of EGTA to buffers completely inhibits antibody staining. Immunohistochemical staining of calmodulin in Dictyostelium cells with MA3-918 results in staining of the contractile vacuoles.

          The MA3-918 antigen is calmodulin purified from Dictyostelium discoideum.

          靶标信息

          Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins by Ca2+. Among the enzymes to be stimulated by the calmodulin-Ca2+ complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis. Calmodulin is a small, highly conserved calcium binding protein found in all eukaryotic cells. With the capacity to bind up to four calcium ions, this 17 kDa protein acts as an important intracellular receptor for regulatory calcium signals. As it binds calcium, calmodulin undergoes conformational changes which can increase its affinity for target proteins. It acts both directly, through interaction with key target enzymes, and indirectly, via specific kinases. Studies have found that calmodulin participates in the regulation of several biological processes including energy and biosynthetic metabolism, cell motility, exocytosis, cytoskeletal assembly, and intracellular modulation of both cAMP and calcium concentrations. Calmodulin is the archetype of the family of calcium-modulated proteins of which nearly 20 members have been found. They are identified by their occurrence in the cytosol or on membranes facing the cytosol and by a high affinity for calcium. Calmodulin contains 149 amino acids and has 4 calcium-binding domains. Its functions include roles in growth and the cell cycle as well as in signal transduction and the synthesis and release of neurotransmitters.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: CALM 1; Calmodulin; Calmodulin 1 (phosphorylase kinase, delta); calmodulin 2 (phosphorylase kinase, delta); calmodulin 3 (phosphorylase kinase, delta); Calmodulin III; Calmodulin-1; Calmodulin-2; Calmodulin-3; calmodulin-like protein; calmodulin-like protein (neoCaM); CaM; CAMI; DD312; epididymis secretory protein Li 72; LP7057 protein; Neo-calmodulin; NeoCaM; phosphorylase kinase delta; phosphorylase kinase subunit delta; phosphorylase kinase, delta subunit; prepro-calmodulin 1; prepro-calmodulin 2; prepro-calmodulin 3

          查看更多 收起

          基因别名: CALM; CALM1; CALM2; CALM3; CALML2; CAM; CAM1; CAM2; CAM3; CAMB; CAMC; CAMI; CAMII; CAMIII; CPVT4; DD132; HEL-S-72; LQT14; LQT15; PHKD; PHKD2; PHKD3; RCJMB04_24e7; RNCAMIII

          查看更多 收起

          UniProt ID: (Chicken) P05419, (Human) P0DP25, (Bovine) P62157, (Chicken) P62149, (Rat) P0DP31

          查看更多 收起

          Entrez Gene ID: (Bovine) 326597, (Chicken) 396523, (Human) 801, (Bovine) 617095, (Bovine) 100297344, (Human) 805, (Chicken) 395855, (Bovine) 520277, (Human) 808, (Rat) 24242, (Rat) 50663, (Rat) 24244

          查看更多 收起

          功能
          calcium ion binding protein binding protein domain specific binding enzyme regulator activity Ras guanyl-nucleotide exchange factor activity adenylate cyclase binding inositol-1,4,5-trisphosphate 3-kinase activity ligand-gated ion channel activity protein kinase binding nitric-oxide synthase regulator activity titin binding type 3 metabotropic glutamate receptor binding thioesterase binding N-terminal myristoylation domain binding phospholipase binding protein serine/threonine kinase activator activity phosphatidylinositol 3-kinase binding ion channel binding calcium-dependent protein binding nitric-oxide synthase binding protein phosphatase activator activity protein C-terminus binding myosin binding CH domain binding disordered domain specific binding calcium channel regulator activity protein N-terminus binding calmodulin-related
          参与通路
          regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum regulation of catalytic activity negative regulation of ryanodine-sensitive calcium-release channel activity positive regulation of ryanodine-sensitive calcium-release channel activity microtubule cytoskeleton organization calcium-mediated signaling G2/M transition of mitotic cell cycle MAPK cascade response to amphetamine regulation of heart rate platelet degranulation detection of calcium ion glycogen catabolic process muscle contraction G-protein coupled receptor signaling pathway activation of adenylate cyclase activity Wnt signaling pathway, calcium modulating pathway positive regulation of peptidyl-threonine phosphorylation negative regulation of peptidyl-threonine phosphorylation regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion substantia nigra development regulation of rhodopsin mediated signaling pathway positive regulation of cyclic nucleotide metabolic process positive regulation of protein autophosphorylation regulation of cytokinesis positive regulation of phosphoprotein phosphatase activity ion transmembrane transport positive regulation of protein dephosphorylation Fc-epsilon receptor signaling pathway positive regulation of DNA binding positive regulation of GTPase activity inositol phosphate metabolic process regulation of nitric-oxide synthase activity positive regulation of nitric-oxide synthase activity positive regulation of cyclic-nucleotide phosphodiesterase activity response to corticosterone response to calcium ion regulation of cardiac muscle contraction positive regulation of protein serine/threonine kinase activity regulation of high voltage-gated calcium channel activity regulation of cell communication by electrical coupling involved in cardiac conduction regulation of ryanodine-sensitive calcium-release channel activity regulation of store-operated calcium channel activity
          It has to be done as per old AB suggested Products section.
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