Invitrogen

Calmodulin Monoclonal Antibody (6D4)

Calmodulin Antibody in Immunocytochemistry (ICC/IF)

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Calmodulin Antibody (MA3-918) in ICC/IF

Immunofluorescence analysis of Calmodulin was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Calmodulin (6D4) Mouse Monoclonal Antibody (Product # MA3-918) at 1:250 dilution in 0.1% BSA and incubated for 3 hours a... View More

Calmodulin Antibody in Western Blot (WB)

Calmodulin Antibody in Immunohistochemistry (IHC)

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产品信息

MA3-918

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

查看5篇已发表文章 5篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组织化学（多聚甲醛固定） (IHC (PFA))

查看1篇已发表文章 1篇已发表文章

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:250

查看2篇已发表文章 2篇已发表文章

酶联免疫吸附实验 (ELISA)

Assay-dependent

产品规格

种属反应

Bacteria, Bovine, Chicken, Human, Rat

已发表种属

Algae, Cnidarian, Coral, Fruit fly, Human, Mouse, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

6D4

抗原

Calmodulin purified from Dictyostelium discoideum.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

purified

保存液

PBS, pH 7.4, with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_325501

产品详细信息

MA3-918 detects calmodulin from bovine, chicken, Chlamydomonas, Dictyostelium, and rat samples. This antibody does not detect parvalbumin, tropinin, S-100, or myosin light chain kinase (MLCK).

MA3-918 has been successfully used in Western blot, immunocytochemistry and ELISA procedures. By Western blot, this antibody detects a 17 kDa protein representing calmodulin from Dictyostelium cell lysate. Addition of EGTA to buffers completely inhibits antibody staining. Immunohistochemical staining of calmodulin in Dictyostelium cells with MA3-918 results in staining of the contractile vacuoles.

The MA3-918 antigen is calmodulin purified from Dictyostelium discoideum.

靶标信息

Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins by Ca2+. Among the enzymes to be stimulated by the calmodulin-Ca2+ complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis. Calmodulin is a small, highly conserved calcium binding protein found in all eukaryotic cells. With the capacity to bind up to four calcium ions, this 17 kDa protein acts as an important intracellular receptor for regulatory calcium signals. As it binds calcium, calmodulin undergoes conformational changes which can increase its affinity for target proteins. It acts both directly, through interaction with key target enzymes, and indirectly, via specific kinases. Studies have found that calmodulin participates in the regulation of several biological processes including energy and biosynthetic metabolism, cell motility, exocytosis, cytoskeletal assembly, and intracellular modulation of both cAMP and calcium concentrations. Calmodulin is the archetype of the family of calcium-modulated proteins of which nearly 20 members have been found. They are identified by their occurrence in the cytosol or on membranes facing the cytosol and by a high affinity for calcium. Calmodulin contains 149 amino acids and has 4 calcium-binding domains. Its functions include roles in growth and the cell cycle as well as in signal transduction and the synthesis and release of neurotransmitters.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Selective inhibition of excitatory synaptic transmission alters the emergent bursting dynamics of in vitro neural networks.

Frontiers in neural circuits

Weir JS,Christiansen N,Sandvig A,Sandvig I

MA3-918 was used in Immunocytochemistry-immunofluorescence to indicate that the disruption in excitatory synaptic transmission during early network development likely affected inhibitory synaptic maturity which resulted in an overall decrease in network inhibition at later stages.

Tue Mar 07 00:00:00 UTC 2023

Corals regulate the distribution and abundance of Symbiodiniaceae and biomolecules in response to changing water depth and sea surface temperature.

Scientific reports

Sivaguru M,Todorov LG,Miller CAH,Fouke CE,Munro CMO,Fouke KW,Fouke KE,Baughman ME,Fouke BW

Published figure using Calmodulin monoclonal antibody (Product # MA3-918) in Immunohistochemistry

Tue Jan 26 00:00:00 UTC 2021

Neuronal calmodulin levels are controlled by CAMTA transcription factors.

eLife

Vuong-Brender TT,Flynn S,Vallis Y,de Bono M

MA3-918 was used in Immunohistochemistry (PFA fixed) to demonstrate that CaM-binding transcription activators mediate a conserved and general mechanism that controls neuronal CaM levels.

Thu Sep 09 00:00:00 UTC 2021

Neuronal calmodulin levels are controlled by CAMTA transcription factors.

eLife

Vuong-Brender TT,Flynn S,Vallis Y,de Bono M

MA3-918 was used in Immunohistochemistry (PFA fixed) to demonstrate that CaM-binding transcription activators mediate a conserved and general mechanism that controls neuronal CaM levels.

Thu Sep 09 00:00:00 UTC 2021

Expression of calcium-buffering proteins in rat intrinsic laryngeal muscles.

Physiological reports

Ferretti R,Marques MJ,Khurana TS,Santo Neto H

MA3-918 was used in western blot to examine calcium-related proteins in the intrinsic laryngeal muscles, cricothyroid, and tibialis anterior muscles from male Sprague-Dawley rats

Mon Jun 01 00:00:00 UTC 2015

Calcium-signaling components in rat insulinoma β-cells (INS-1) and pancreatic islets are differentially influenced by melatonin.

Journal of pineal research

Bazwinsky-Wutschke I,Mühlbauer E,Albrecht E,Peschke E

MA3-918 was used in immunocytochemistry to study the differential effects of melatonin on the calcium signaling pathways of INS1 insulinoma cells and pancreatic islets

Thu May 01 00:00:00 UTC 2014

Roles of calmodulin and calcium/calmodulin-dependent protein kinase in flagellar motility regulation in the coral Acropora digitifera.

Marine biotechnology (New York, N.Y.)

Morita M,Iguchi A,Takemura A

MA3-918 was used in western blot to investigate the involvement of calmodulin and calcium/calmodulin-dependent protein kinase in the sperm flagellar motility regulation of Acropora Digitifera

Tue Apr 07 00:00:00 UTC 2009

Regulation of sperm flagellar motility activation and chemotaxis caused by egg-derived substance(s) in sea cucumber.

Cell motility and the cytoskeleton

Morita M,Kitamura M,Nakajima A,Sri Susilo E,Takemura A,Okuno M

MA3-918 was used in western blot to investigate the effect of egg-derived substances on sperm flagellar motility activation and chemotaxis

Wed Apr 01 00:00:00 UTC 2009

Increased sensitivity of the ryanodine receptor to halothane-induced oligomerization in malignant hyperthermia-susceptible human skeletal muscle.

Journal of applied physiology (Bethesda, Md. : 1985)

Glover L,Heffron JJ,Ohlendieck K

MA3-918 was used in western blot to investigate the importance of RyR2 complex formation in the development of a metabolic crisis in malignant hyperthermia

Thu Jan 01 00:00:00 UTC 2004

Characterization of the calcium-binding contractile protein centrin from Tetraselmis striata (Pleurastrophyceae).

The Journal of protozoology

Coling DE,Salisbury JL

MA3-918 was used in western blot to investigate the biochemical properties of centrin purified from Tetraselmis striata

Tue Sep 01 00:00:00 UTC 1992

生物信息学

蛋白别名: CALM 1; Calmodulin; Calmodulin 1 (phosphorylase kinase, delta); calmodulin 2 (phosphorylase kinase, delta); calmodulin 3 (phosphorylase kinase, delta); Calmodulin III; Calmodulin-1; Calmodulin-2; Calmodulin-3; calmodulin-like protein; calmodulin-like protein (neoCaM); CaM; CAMI; DD312; epididymis secretory protein Li 72; LP7057 protein; Neo-calmodulin; NeoCaM; phosphorylase kinase delta; phosphorylase kinase subunit delta; phosphorylase kinase, delta subunit; prepro-calmodulin 1; prepro-calmodulin 2; prepro-calmodulin 3

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基因别名: CALM; CALM1; CALM2; CALM3; CALML2; CAM; CAM1; CAM2; CAM3; CAMB; CAMC; CAMI; CAMII; CAMIII; CPVT4; DD132; HEL-S-72; LQT14; LQT15; PHKD; PHKD2; PHKD3; RCJMB04_24e7; RNCAMIII

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功能

calcium ion binding protein binding protein domain specific binding enzyme regulator activity Ras guanyl-nucleotide exchange factor activity adenylate cyclase binding inositol-1,4,5-trisphosphate 3-kinase activity ligand-gated ion channel activity protein kinase binding nitric-oxide synthase regulator activity titin binding type 3 metabotropic glutamate receptor binding thioesterase binding N-terminal myristoylation domain binding phospholipase binding protein serine/threonine kinase activator activity phosphatidylinositol 3-kinase binding ion channel binding calcium-dependent protein binding nitric-oxide synthase binding protein phosphatase activator activity protein C-terminus binding myosin binding CH domain binding disordered domain specific binding calcium channel regulator activity protein N-terminus binding calmodulin-related

参与通路

Calmodulin Monoclonal Antibody (6D4)

Calmodulin Antibody (MA3-918) in ICC/IF

产品信息

MA3-918

免疫印迹 (WB)

免疫组化 (IHC)

免疫组织化学（多聚甲醛固定） (IHC (PFA))

免疫组化（冰冻） (IHC (F))

免疫细胞化学 (ICC/IF)

酶联免疫吸附实验 (ELISA)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

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