Invitrogen

Calreticulin Polyclonal Antibody

Calreticulin Antibody in Western Blot (WB)

图: 1 / 10

Calreticulin Antibody (PA1-903) in WB

Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of MCF7 (Lane 1), MDA-MB-231 (Lane 2), HeLa (Lane 3), K-562 (Lane 4), Jurkat (Lane 5) and A549 (Lane 6). The blot was probed with Chicken Anti-Calreticulin Polyclonal Antibody (Product # PA1-903, 1:2000 dilution) and detected by chemiluminescence using Goat anti-Chicken IgY (H+L) Secondary Antibody, HRP conjugate (Product # A16054, 0.4 µg/mL, 1:2500 dilution). A 55 kDa band corresponding to Calreticulin was observed across the cell lines tested. Known quantity... View More

Product Image Gallery

产品信息

PA1-903

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

查看12篇已发表文章 12篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

Assay-dependent

查看2篇已发表文章 2篇已发表文章

免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Dog, Hamster, Human, Mouse, Rabbit, Rat

已发表种属

Amphibian, Human, Mouse, Rabbit, Rat

宿主/亚型

Chicken / IgY

分类

Polyclonal

类型

Antibody

抗原

Synthetic Peptide: D(399) E K E E D E E E S P G Q A K D(414).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2228457

产品详细信息

PA1-903 detects calreticulin from human, rat, mouse, hamster, rabbit and canine tissues.

PA1-903 has been successfully used in Western blot, immunoprecipitation, immunocytochemistry and immunofluorescence procedures. By Western blot, this antibody detects an ~60 kDa protein representing calreticulin and an ~120 kDa unidentified protein from rat brain extract.

PA1-903 immunizing peptide corresponds to amino acid residues 399-414 from mouse calreticulin. PA1-903 immunizing peptide (Cat. # PEP-017) is available for use in neutralization and control experiments.

靶标信息

Calr or Calreticulin is a calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. Calr, a lectin, interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Calr also interacts with with the DNA-binding domain of NR3C1 and mediates its nuclear export. It may also be involved in maternal gene expression regulation and oocyte maturation via the regulation of calcium homeostasis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Insulin Treatment Reduces Susceptibility to Atrial Fibrillation in Type 1 Diabetic Mice.

Frontiers in cardiovascular medicine

Maria Z,Campolo AR,Scherlag BJ,Ritchey JW,Lacombe VA

PA1-903 was used in Western Blotting to suggest that insulin and glucose disturbances are sufficient to induce atrial fibrillation (AF) susceptibility during mild diabetes.

Mon Sep 28 00:00:00 UTC 2020

Insulin Treatment Reduces Susceptibility to Atrial Fibrillation in Type 1 Diabetic Mice.

Frontiers in cardiovascular medicine

Maria Z,Campolo AR,Scherlag BJ,Ritchey JW,Lacombe VA

PA1-903 was used in Western Blotting to suggest that insulin and glucose disturbances are sufficient to induce atrial fibrillation (AF) susceptibility during mild diabetes.

Mon Sep 28 00:00:00 UTC 2020

Glial Growth Factor 2 Regulates Glucose Transport in Healthy Cardiac Myocytes and During Myocardial Infarction via an Akt-Dependent Pathway.

Frontiers in physiology

Shoop S,Maria Z,Campolo A,Rashdan N,Martin D,Lovern P,Lacombe VA

PA1-903 was used in Western Blotting to investigate whether treatment with a version of glial growth factor 2 will enhance glucose transport in the healthy myocardium and during myocardial infarction.

Wed Sep 30 00:00:00 UTC 2020

Diabetes Alters the Expression and Translocation of the Insulin-Sensitive Glucose Transporters 4 and 8 in the Atria.

PloS one

Maria Z,Campolo AR,Lacombe VA

Published figure using Calreticulin polyclonal antibody (Product # PA1-903) in Western Blot

Tue Jun 28 00:00:00 UTC 2016

Gestational exposure to diethylstilbestrol alters cardiac structure/function, protein expression and DNA methylation in adult male mice progeny.

Toxicology and applied pharmacology

Haddad R,Kasneci A,Mepham K,Sebag IA,Chalifour LE

PA1-903 was used in western blot to study the effects of diethylstilbestrol exposure during gestation on epigenetic DNA modifications and cardiac structure and function in adult mice

Tue Jan 01 00:00:00 UTC 2013

Bilateral acute pyogenic conjunctivitis with iritis induced by unilateral topical application of bacterial peptidoglycan muramyl dipeptide in adult rabbits.

Experimental eye research

Langford MP,Foreman BD,Srur L,Ganley JP,Redens TB

PA1-903 was used in immunohistochemistry to study the role of bacterial peptidoglycan muramyl dipeptide in the pathogenesis of acute pyogenic conjunctivitis and iritis

Fri Nov 01 00:00:00 UTC 2013

mTOR-Controlled Autophagy Requires Intracellular Ca(2+) Signaling.

PloS one

Decuypere JP,Kindt D,Luyten T,Welkenhuyzen K,Missiaen L,De Smedt H,Bultynck G,Parys JB

PA1-903 was used in western blot to study the role of intracellular Ca(2+) signaling in mTOR-modulated autophagy

Mon Sep 23 00:00:00 UTC 2013

Ins(1,4,5)P3 receptor-mediated Ca2+ signaling and autophagy induction are interrelated.

Autophagy

Decuypere JP,Welkenhuyzen K,Luyten T,Ponsaerts R,Dewaele M,Molgó J,Agostinis P,Missiaen L,De Smedt H,Parys JB,Bultynck G

PA1-903 was used in western blot to study the relationship between Ins(1,4,5)P3 receptor-mediated Ca2+ signaling and the induction of autophagy

Thu Dec 01 00:00:00 UTC 2011

Comparative proteomics profiling of a phospholamban mutant mouse model of dilated cardiomyopathy reveals progressive intracellular stress responses.

Molecular & cellular proteomics : MCP

Gramolini AO,Kislinger T,Alikhani-Koopaei R,Fong V,Thompson NJ,Isserlin R,Sharma P,Oudit GY,Trivieri MG,Fagan A,Kannan A,Higgins DG,Huedig H,Hess G,Arab S,Seidman JG,Seidman CE,Frey B,Perry M,Backx PH,Liu PP,MacLennan DH,Emili A

PA1-903 was used in western blot to perform the global proteomics surveys of cardiac ventricle of a phospholamban mutant mouse

Sat Mar 01 00:00:00 UTC 2008

Cardiac mitochondrial connexin 43 regulates apoptosis.

Biochemical and biophysical research communications

Goubaeva F,Mikami M,Giardina S,Ding B,Abe J,Yang J

PA1-903 was used in western blot to investigate the role of cardiac mitochondrial connexin 43 in apoptosis

Fri Jan 05 00:00:00 UTC 2007

Localization of the iron transport proteins Mobilferrin and DMT-1 in the duodenum: the surprising role of mucin.

American journal of hematology

Simovich M,Hainsworth LN,Fields PA,Umbreit JN,Conrad ME

PA1-903 was used in western blot to study the intestinal iron uptake and the involvement of mucin

Mon Sep 01 00:00:00 UTC 2003

C1q and mannose binding lectin engagement of cell surface calreticulin and CD91 initiates macropinocytosis and uptake of apoptotic cells.

The Journal of experimental medicine

Ogden CA,deCathelineau A,Hoffmann PR,Bratton D,Ghebrehiwet B,Fadok VA,Henson PM

PA1-903 was used in immunocytochemistry to study the mechanisms of macropinocytosis and apoptotic cell removal

Mon Sep 17 00:00:00 UTC 2001

Association of ERp57 with mouse MHC class I molecules is tapasin dependent and mimics that of calreticulin and not calnexin.

Journal of immunology (Baltimore, Md. : 1950)

Harris MR,Lybarger L,Yu YY,Myers NB,Hansen TH

PA1-903 was used in western blot to study the interaction between ERp57 and mouse MHC class I molecules.

Fri Jun 01 00:00:00 UTC 2001

Interactions of HLA-B27 with the peptide loading complex as revealed by heavy chain mutations.

International immunology

Harris MR,Lybarger L,Myers NB,Hilbert C,Solheim JC,Hansen TH,Yu YY

PA1-903 was used in western blot to investigate how HLA-B27 interact with the peptide loading complex

Mon Oct 01 00:00:00 UTC 2001

Calreticulin inhibits repetitive intracellular Ca2+ waves.

Cell

Camacho P,Lechleiter JD

PA1-903 was used in immunocytochemistry to investigate the role of CRT in IP3-mediated caclium signaling

Fri Sep 08 00:00:00 UTC 1995

Chaperone function of calreticulin when expressed in the endoplasmic reticulum as the membrane-anchored and soluble forms.

The Journal of biological chemistry

Wada I,Imai S,Kai M,Sakane F,Kanoh H

PA1-903 was used in western blot to demonstrate that calreticulin in ER is another novel CAS-sensitive chaperone

Fri Sep 01 00:00:00 UTC 1995

生物信息学

蛋白别名: Autoantigen RO; CABP3; CALBP; Calcium-binding protein 3; Calregulin; Calreticulin; calreticulin precursor; CRP55; Endoplasmic reticulum resident protein 60; epididymis secretory sperm binding protein Li 99n; ERp60; FLJ26680; grp60; HACBP; Ro/SS-A Antigen; Sicca syndrome antigen A (autoantigen Ro; calreticulin)

查看更多收起

基因别名: CALR; Calregulin; cC1qR; CRP55; CRT; CRTC; ERp60; HACBP; HEL-S-99n; I79_008346; RO; SSA