Invitrogen

Carbonic Anhydrase IX Monoclonal Antibody (GT12)

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

1个已发表图片

3 篇参考文献

查看其他22个Carbonic Anhydrase IX抗体

说明书

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说明书

实验方案

技术支持

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Carbonic Anhydrase IX Antibody in Immunocytochemistry (ICC/IF) — Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431 cells treated with 200 µM CoCl₂ for 48hr using anti-CAIX antibody [GT12] (Product # MA5-16318) at a dilution of 1:1,000.

Carbonic Anhydrase IX Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Carbonic Anhydrase IX Monoclonal Antibody (GT12) detects Carbonic Anhydrase IX protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. Carbonic Anhydrase IX stained by Carbonic Anhydrase IX Monoclonal Antibody (GT12) (Product # MA5-16318) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Carbonic Anhydrase IX Antibody in Western Blot (WB) — Western blot was performed using Anti-Carbonic Anhydrase IX Monoclonal Antibody (Product # MA5-16318) and ~ 50-55 kDa bands corresponding to CA9 was observed across cell lines tested and increased upon Deferoxamine and Cobalt Chloride treatment in HeLa along with an uncharacterized band (*) at ~32 kDa. Whole cell extracts (30 µg lysate) of U-87 MG (Lane 1), HeLa (Lane 2), HeLa treated with Deferoxamine (100uM for 24 Hours) (Lane 3) and HeLa treated with Cobalt Chloride (200uM for 24 Hours) (Lane 4) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Carbonic Anhydrase IX Antibody in Flow Cytometry (Flow) — Flow cytometry on HeLa cells (1x10^6) stained with Carbonic Anhydrase IX Monoclonal Antibody (GT12) (Product # MA5-16318) at a 1:1,000 dilution. HeLa cells were untreated (green) or treated with 200 µM CoCl2 (pink) for 48 hr.

Carbonic Anhydrase IX Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models tested owing to their inherent genetic constitution. Higher expression of CA9 was observed in U-87 MG in comparison to HeLa using Anti-Carbonic Anhydrase IX Monoclonal Antibody (GT12) (Product # MA5-16318) in Western Blot. {RE}

产品信息

MA5-16318

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:100-1:1,000	查看1篇已发表文章 1篇已发表文章
免疫组化 (IHC)	-	查看2篇已发表文章 2篇已发表文章
免疫组化（石蜡） (IHC (P))	1:100-1:1,000	-
免疫组化（冰冻） (IHC (F))	Assay-dependent	-
免疫细胞化学 (ICC/IF)	1:100-1:1,000	-
流式细胞分析 (Flow)	1:100-1:1,000	-
免疫沉淀 (IP)	2-10 µg/mg of lysate	-

产品规格
种属反应	Human
已发表种属	Human
宿主/亚型	Mouse / IgG2b
分类	Monoclonal
类型	Antibody
克隆号	GT12
抗原	linear repetitive epitope in the PG region and allows for selective detection of both native and denatured Human CA IX.
偶联物	Unconjugated
形式	Liquid
浓度	0.1 mg/mL
纯化类型	Protein G
保存液	PBS
内含物	no preservative
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2537837

产品详细信息

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Carbonic anhydrase (CA) is an enzyme that assists rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in all mammalian tissues. There are many genes that are inducible by hypoxia, via HIF-1 alpha. CA IX is one of the most inducible genes because of its stability and location within the membrane. Carbonic anhydrases have a widespread role in regulating pH in normal tissues, by regulating hydrogen ion (H+) flux. The pH is important in cell death under hypoxia, thus a blockade of CA IX results in increased cell death under hypoxia. Therefore, CA IX has become a reliable histochemical marker of hypoxia.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Hypoxia-induced autophagy drives colorectal cancer initiation and progression by activating the PRKC/PKC-EZR (ezrin) pathway.

Autophagy

Qureshi-Baig K,Kuhn D,Viry E,Pozdeev VI,Schmitz M,Rodriguez F,Ullmann P,Koncina E,Nurmik M,Frasquilho S,Nazarov PV,Zuegel N,Boulmont M,Karapetyan Y,Antunes L,Val D,Mittelbronn M,Janji B,Haan S,Letellier E

MA5-16318 was used in Immunohistochemistry to observe significantly reduced in vivo tumor initiation and growth after a stable knockdown of ATG5.

Sat Aug 01 00:00:00 EDT 2020

Linker stability influences the anti-tumor activity of acetazolamide-drug conjugates for the therapy of renal cell carcinoma.

Journal of controlled release : official journal of the Controlled Release Society

Cazzamalli S,Corso AD,Neri D

MA5-16318 was used in Immunohistochemistry-immunofluorescence to show that small molecule-drug conjugate (SMDC) products, obtained by the coupling of acetazolamide with monomethyl auristatin E (MMAE) using dipeptide linkers, display a potent anti-tumoral activity in mice bearing xenografted SKRC-52 renal cell carcinomas.

Sat Jan 28 00:00:00 EST 2017

Mineralization of bone-related SaOS-2 cells under physiological hypoxic conditions.

The FEBS journal

Müller WE,Schröder HC,Tolba E,Diehl-Seifert B,Wang X

MA5-16318 was used in blocking or activating experiment and western blot to examine the metabolism of hypoxic SaOS-2 cells

Fri Jan 01 00:00:00 EST 2016

生物信息学

蛋白别名: CA-9; CA-IX; Carbonate dehydratase IX; Carbonic anhydrase 9; Carbonic anhydrase IX; carbonic dehydratase; Membrane antigen MN; P54 / 58N; P54/58N; pMW1; RCC-associated antigen G250; RCC-associated protein G250; Renal cell carcinoma-associated antigen G250

基因别名: CA9; CAIX; G250; MN

UniProt ID: (Human) Q16790

Entrez Gene ID: (Human) 768

功能

morphogenesis of an epithelium one-carbon metabolic process bicarbonate transport response to testosterone response to drug secretion regulation of transcription from RNA polymerase II promoter in response to hypoxia

参与通路

carbonate dehydratase activity zinc ion binding

Carbonic Anhydrase IX Monoclonal Antibody (GT12)

产品信息

MA5-16318

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫组化（冰冻） (IHC (F))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

免疫沉淀 (IP)

种属反应

已发表种属

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分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

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生物信息学

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