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Invitrogen
Cas12j Monoclonal Antibody (5F95)
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Cas12j Antibody (MA5-47448) in ICC/IF
Immunofluorescent analysis of Cas12j in HEK293 cells. Samples were stained with Cas12j monoclonal antibody (Product # MA5-47448) using a dilution of 1:2,000 (red). Cells were transfected with pCI-Neo-Mod vector including DNA encoding the Cas theta protein. Costained with rabbit pAb to vimentin dilution 1:2,000, in green. The blue is Hoechst staining of nuclear DNA. The cas12j antibody reveals expression of the Cas theta protein only in transfected cells. The vimentin antibody stains the cytoskeletal intermediate filament network in all cel... View More
产品信息
MA5-47448
产品规格
种属反应
Chemical
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
5F95
抗原
Recombinant Cas theta/Cas-12j expressed in and purified from E. coli
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS with 50% glycerol
内含物
5mM sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
靶标信息
There has been much recent interest in gene editing and other genetic manipulations by CRISPR-Cas family enzymes. The lab of Nobel prize winner Jennifer Doudna characterized these Cas enzymes and showed that they could be used to perform CRISPR, but had the significant advantage that they were much smaller in molecular size than other Cas family enzymes such as the widely used Cas9 enzymes from S. pyogenes and S. aureus. One of these enzymes is referred to as Cas theta and also known as Cas-12j. The use of such smaller Cas family enzymes leaves more room for other nucleic acid sequences in AAV and other viral vectors which typically have a limited DNA capacity, thus allowing more versatility for new CRISPR based manipulations.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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