Invitrogen

Caspase 3 Polyclonal Antibody

Caspase 3 Antibody in Immunocytochemistry (ICC/IF)

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Caspase 3 Antibody (PA5-77887) in ICC/IF

Immunofluorescence analysis of Caspase-3 was performed using 70% confluent log phase NTERA-2 cl.D1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Caspase 3 Polyclonal Antibody (Product # PA5-77887) at 1:200 dilution in 0.1% BSA, incubated at 4 de... View More

Caspase 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Caspase 3 Antibody in Immunoprecipitation (IP)

Caspase 3 Antibody in Immunohistochemistry (IHC)

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产品信息

PA5-77887

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:10,000

查看2篇已发表文章 2篇已发表文章

免疫组化 (IHC)

查看10篇已发表文章 10篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

查看1篇已发表文章 1篇已发表文章

免疫组化（冰冻） (IHC (F))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

免疫沉淀 (IP)

1:100-1:500

点杂交 (DB)

Assay-dependent

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the center region of human Caspase 3. The exact sequence is proprietary.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.15 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2735574

产品详细信息

Positive Control: HeLa, HeLa (30 µM Cisplatin treatment for 24 hr), PC-12 (30uM cisplatin treatment for 24h), PC-12, Rat2, C8D30, NIH-3T3, Raw264.7, C2C12, Jurkat, Raji, NCI-H929, K562, THP-1, HL-60

Predicted Reactivity: Dog (89%), Cat (87%), Pig (88%), Rabbit (86%), Chimpanzee (99%), Bovine (84%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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PA5-77887 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to provide a new way to passively target mitochondria for AKI, and give inspiration for the treatment of many major diseases closely related to mitochondria, such as myocardial infarction and cerebral infarction.

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Protective Effects of Taif Rosewater Against Testicular Impairment Induced By Lead Intoxication In Rats.

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Retinal Protection from LED-Backlit Screen Lights by Short Wavelength Absorption Filters.

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Published figure using Caspase 3 polyclonal antibody (Product # PA5-77887) in Immunohistochemistry (Paraffin)

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Published figure using Caspase 3 polyclonal antibody (Product # PA5-77887) in Immunohistochemistry (Paraffin)

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生物信息学

蛋白别名: Apopain; Apopain precursor; CASP-3; Caspase 3 apoptosis related cysteine protease (ICE-like cysteine protease); caspase 3, apoptosis related cysteine protease; Caspase 3, apoptosis related cysteine protease (ICE-like cysteine protease); caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; Caspase-3; Caspase3; CPP-32; CPP32beta; Cysteine protease CPP32; ICE3; IRP; LICE; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; SREBP cleavage activity 1; yama protein

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基因别名: A830040C14Rik; AC-3; Apopain; CASP-3; CASP3; Caspase-3; CC3; CPP-32; CPP32; CPP32B; Lice; mldy; SCA-1; Yama

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UniProt ID: (Human) P42574, (Mouse) P70677, (Rat) P55213

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Entrez Gene ID: (Human) 836, (Mouse) 12367, (Rat) 25402

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功能