Invitrogen

Caspase 3 (Cleaved Asp175) Polyclonal Antibody

17个已发表图片

21 篇参考文献

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (6)
已发表图片 (17)

Caspase 3 (Cleaved Asp175) Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 23

Caspase 3 (Cleaved Asp175) Antibody (PA5-114687) in ICC/IF

Immunocytochemistry analysis of Caspase 3 (Cleaved Asp175) in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114687) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 ... View More

Caspase 3 (Cleaved Asp175) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Caspase 3 (Cleaved Asp175) Antibody in Western Blot (WB)

Caspase 3 (Cleaved Asp175) Antibody in Immunohistochemistry (IHC)

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产品信息

PA5-114687

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

查看12篇已发表文章 12篇已发表文章

免疫组化 (IHC)

查看7篇已发表文章 7篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:100-1:500

查看1篇已发表文章 1篇已发表文章

酶联免疫吸附实验 (ELISA)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human CASP3(Accession P42574), corresponding to amino acid residues C163-M182.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2899323

产品详细信息

Antibody detects endogenous levels of fragment of activated Caspase 3 resulting from cleavage adjacent to Asp175.

靶标信息

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

MicroRNA‑203a‑3p improves bleomycin and pingyangmycin sensitivity by inactivating the PI3K/AKT pathway in hemangioma.

Experimental and therapeutic medicine

Zhuo L,Hu Z,Chang J,Guo Q,Guo J

Published figure using Caspase 3 (Cleaved Asp175) polyclonal antibody (Product # PA5-114687) in Western Blot

Thu Feb 01 00:00:00 UTC 2024

Lysine demethylase 5C inhibits transcription of prefoldin subunit 5 to activate c-Myc signal transduction and colorectal cancer progression.

Molecular medicine (Cambridge, Mass.)

Yu F,Li L,Gu Y,Wang S,Zhou L,Cheng X,Jiang H,Huang Y,Zhang Y,Qian W,Li X,Liu Z

Published figure using Caspase 3 (Cleaved Asp175) polyclonal antibody (Product # PA5-114687) in Immunohistochemistry

Fri Jan 12 00:00:00 UTC 2024

Propofol synergizes with circAPBB2 to protect against hypoxia/reoxygenation-induced oxidative stress, inflammation, and apoptosis of human cardiomyocytes.

Immunity, inflammation and disease

Jin C,Yuan S,Piao L,Ren M,Liu Q

Published figure using Caspase 3 (Cleaved Asp175) polyclonal antibody (Product # PA5-114687) in Western Blot

Tue Aug 01 00:00:00 UTC 2023

CircRANBP17 modulated KDM1A to regulate neuroblastoma progression by sponging miR-27b-3p.

Open medicine (Warsaw, Poland)

Zhao L,Fan J,Zhang C,Zhang Z,Dong J

Published figure using Caspase 3 (Cleaved Asp175) polyclonal antibody (Product # PA5-114687) in Western Blot

Wed Mar 22 00:00:00 UTC 2023

Granulocyte-colony stimulating factor ameliorates di-ethylhexyl phthalate-induced cardiac muscle injury via stem cells recruitment, Desmin protein regulation, antifibrotic and antiapoptotic mechanisms.

Journal of molecular histology

Abdelrahman SA,Khattab MA,Youssef MS,Mahmoud AA

PA5-114687 was used in Immunohistochemistry to examine in depth the effect of DEHP on the histological and biochemical structure of the cardiac muscle in adult male albino rats and the mechanisms underlying the possible ameliorative effect of G-CSF.

Tue Aug 01 00:00:00 UTC 2023

miR-506-3p Relieves Neuropathic Pain following Brachial Plexus Avulsion via Mitigating Microglial Activation through Targeting the CCL2-CCR2 Axis.

Developmental neuroscience

Jin X,Zheng W,Chi S,Cui T,He W

PA5-114687 was used in Immunohistochemistry to show that miR-506-3p had neuroprotective and inflammation-fighting functions in the tBPI rat model via CCL2/CCR2/NF-κB axis.

Fri Mar 10 00:00:00 UTC 2023

ADAM metallopeptidase domain 10 knockdown enables podocytes to resist high glucose stimulation by inhibiting pyroptosis via MAPK pathway.

Experimental and therapeutic medicine

Sui C,Zhou D

PA5-114687 was used in Western Blotting to demonstrate that ADAM10 knockdown suppressed the inflammation, apoptosis and pyroptosis of HG-stimulated podocytes by blocking the MAPK signaling pathway.

Thu Jun 01 00:00:00 UTC 2023

miR-340-5p inhibits pancreatic acinar cell inflammation and apoptosis via targeted inhibition of HMGB1.

Experimental and therapeutic medicine

Gao Y,Wang L,Niu Z,Feng H,Liu J,Sun J,Gao Y,Pan L

PA5-114687 was used in Western Blot to present that miR-340-5p may be a useful strategy in AP via upregulation of HMGB1 and subsequent promotion of inflammation and apoptosis.

Tue Feb 01 00:00:00 UTC 2022

Purine nucleoside phosphorylase deficiency induces p53-mediated intrinsic apoptosis in human induced pluripotent stem cell-derived neurons.

Scientific reports

Tsui M,Biro J,Chan J,Min W,Dobbs K,Notarangelo LD,Grunebaum E

Published figure using Caspase 3 (Cleaved Asp175) polyclonal antibody (Product # PA5-114687) in Immunocytochemistry

Tue May 31 00:00:00 UTC 2022

Purine nucleoside phosphorylase deficiency induces p53-mediated intrinsic apoptosis in human induced pluripotent stem cell-derived neurons.

Scientific reports

Tsui M,Biro J,Chan J,Min W,Dobbs K,Notarangelo LD,Grunebaum E

Published figure using Caspase 3 (Cleaved Asp175) polyclonal antibody (Product # PA5-114687) in Immunocytochemistry

Tue May 31 00:00:00 UTC 2022

Penta-O-Galloyl-β-D-Glucose in Pistacia integerrima Targets AMPK-ULK1 and ERK/STAT3 Signaling Axes to Induce ROS-Independent Autophagic Cell Death in Human Lung Cancer Cells.

Frontiers in pharmacology

Balkrishna A,Mulay VP,Verma S,Srivastava J,Lochab S,Varshney A

Published figure using Caspase 3 (Cleaved Asp175) polyclonal antibody (Product # PA5-114687) in Western Blot

Sat Aug 06 00:00:00 UTC 2022

Penta-O-Galloyl-β-D-Glucose in Pistacia integerrima Targets AMPK-ULK1 and ERK/STAT3 Signaling Axes to Induce ROS-Independent Autophagic Cell Death in Human Lung Cancer Cells.

Frontiers in pharmacology

Balkrishna A,Mulay VP,Verma S,Srivastava J,Lochab S,Varshney A

Published figure using Caspase 3 (Cleaved Asp175) polyclonal antibody (Product # PA5-114687) in Western Blot

Sat Aug 06 00:00:00 UTC 2022

Yi-Shen-Hua-Shi granules inhibit diabetic nephropathy by ameliorating podocyte injury induced by macrophage-derived exosomes.

Frontiers in pharmacology

Liang M,Zhu X,Zhang D,He W,Zhang J,Yuan S,He Q,Jin J

Published figure using Caspase 3 (Cleaved Asp175) polyclonal antibody (Product # PA5-114687) in Immunohistochemistry

Wed Dec 21 00:00:00 UTC 2022

Survival of Stem Cells and Progenitors in the Intestine Is Regulated by LPA₅-Dependent Signaling.

Cellular and molecular gastroenterology and hepatology

Liang Z,He P,Han Y,Yun CC

PA5-114687 was used in Immunocytochemistry, Immunohistochemistry (PFA fixed), Western Blot to identify LPA5 receptor as a potent regulator of the survival of stem cells and transit-amplifying cells in the intestine.

Mon Jun 20 00:00:00 UTC 2022

Nickel's Role in Pancreatic Ductal Adenocarcinoma: Potential Involvement of microRNAs.

Toxics

Mortoglou M,Manić L,Buha Djordjevic A,Bulat Z,Đorđević V,Manis K,Valle E,York L,Wallace D,Uysal-Onganer P

PA5-114687 was used in ELISA to indicate that the environmental pollutant Ni plays an important role in the progression of PDAC by promoting an association between miRs and Ni exposure during PDAC pathogenesis.

Mon Mar 21 00:00:00 UTC 2022

Quercetin Ameliorates Testicular Damage in Zucker Diabetic Fatty Rats through Its Antioxidant, Anti-Inflammatory and Anti-Apoptotic Properties.

International journal of molecular sciences

Tvrdá E,Kováč J,Ferenczyová K,Kaločayová B,¿uračka M,Benko F,Almášiová V,Barteková M

PA5-114687 was used in Western Blot to suggest that QUE is a potentially beneficial agent to reduce testicular damage in ZDF rats with Type 2 diabetes mellitus by decreasing oxidative stress, chronic inflammation, and excessive cell loss through apoptosis.

Fri Dec 16 00:00:00 UTC 2022

Ameliorative Effect of Curcumin Nanoparticles against Monosodium Iodoacetate-Induced Knee Osteoarthritis in Rats.

Mediators of inflammation

Hamdalla HM,Ahmed RR,Galaly SR,Naguib IA,Alghamdi BS,Ahmed OM,Farghali A,Abdul-Hamid M

PA5-114687 was used in Immunocytochemistry, Immunohistochemistry (PFA fixed), Western Blot to demonstrate that CUR NPs provides effective protection against MIA-induced OA and knee joint histological deteriorated changes due to its anti-inflammatory, antioxidant, and antiapoptotic properties.

Fri Dec 30 00:00:00 UTC 2022

Pharmacological Disruption of Phosphorylated Eukaryotic Initiation Factor-2α/Activating Transcription Factor 4/Indian Hedgehog Protects Intervertebral Disc Degeneration via Reducing the Reactive Oxygen Species and Apoptosis of Nucleus Pulposus Cells.

Frontiers in cell and developmental biology

Bao J,Qian Z,Liu L,Hong X,Che H,Wu X

Published figure using Caspase 3 (Cleaved Asp175) polyclonal antibody (Product # PA5-114687) in Western Blot

Fri Jun 25 00:00:00 UTC 2021

Frontiers in cell and developmental biology

Bao J,Qian Z,Liu L,Hong X,Che H,Wu X

Published figure using Caspase 3 (Cleaved Asp175) polyclonal antibody (Product # PA5-114687) in Western Blot

Fri Jun 25 00:00:00 UTC 2021

Frontiers in cell and developmental biology

Bao J,Qian Z,Liu L,Hong X,Che H,Wu X

Published figure using Caspase 3 (Cleaved Asp175) polyclonal antibody (Product # PA5-114687) in Western Blot

Fri Jun 25 00:00:00 UTC 2021

Frontiers in cell and developmental biology

Bao J,Qian Z,Liu L,Hong X,Che H,Wu X

Published figure using Caspase 3 (Cleaved Asp175) polyclonal antibody (Product # PA5-114687) in Western Blot

Fri Jun 25 00:00:00 UTC 2021

LncRNA HOXA11-AS aggravates the keloid formation by targeting miR-148b-3p/IGFBP5 axis.

Biochemical and biophysical research communications

Wang J,Shen J

PA5-114687 was used in Western Blot to show keloid progression can be promoted by long non-coding RNA homeobox HOXA11-AS.

Fri Dec 03 00:00:00 UTC 2021

Effects of lncRNA ANRIL-knockdown on the proliferation, apoptosis and cell cycle of gastric cancer cells.

Oncology letters

Hu X,Lou T,Yuan C,Wang Y,Tu X,Wang Y,Zhang T

PA5-114687 was used in Western Blotting to construct drug-resistant gastric cancer cell lines using the stepwise continuous selection method.

Sun Aug 01 00:00:00 UTC 2021

PAD4 inhibitor promotes DNA damage and radiosensitivity of nasopharyngeal carcinoma cells.

Environmental toxicology

Chen H,Wei L,Luo M,Wang X,Zhan Y,Mao Y,Huang C,Li J,Lu H

PA5-114687 was used in Immunocytochemistry, Immunohistochemistry (PFA fixed), Western Blot to determine the effects of YW3-56 on the radiosensitivity of NPC cells.

Mon Nov 01 00:00:00 UTC 2021

PAX8-AS1 knockdown facilitates cell growth and inactivates autophagy in osteoblasts via the miR-1252-5p/GNB1 axis in osteoporosis.

Experimental & molecular medicine

Huang C,Li R,Yang C,Ding R,Li Q,Xie D,Zhang R,Qiu Y

PA5-114687 was used in Western Blotting to determine the specific role of PAX8-AS1 in osteoporosis.

Sat May 01 00:00:00 UTC 2021

生物信息学

蛋白别名: Apopain; CASP-3; Caspase 3 apoptosis related cysteine protease (ICE-like cysteine protease); caspase 3, apoptosis related cysteine protease; Caspase 3, apoptosis related cysteine protease (ICE-like cysteine protease); caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; Caspase-3; CPP-32; CPP32beta; Cysteine protease CPP32; IRP; LICE; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; SREBP cleavage activity 1; Yama; yama protein

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基因别名: A830040C14Rik; AC-3; Apopain; CASP-3; CASP3; Caspase-3; CC3; CPP-32; CPP32; CPP32B; Lice; mldy; SCA-1; Yama

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UniProt ID: (Human) P42574, (Mouse) P70677, (Rat) P55213

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Entrez Gene ID: (Human) 836, (Mouse) 12367, (Rat) 25402

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功能