Invitrogen

Cdc25A Polyclonal Antibody

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Cdc25A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Cdc25A in paraffin-embedded rat pancreas. Sample was incubated with Cdc25A polyclonal antibody (Product # PA5-77902) using a dilution of 1:500.

Cdc25A Antibody in Western Blot (WB) — Western blot analysis of Cdc25A in A) H1299, B) MCF-7 cells using 30 µg of protein. Samples were separated with 7.5% SDS-PAGE and incubated with Cdc25A polyclonal antibody (Product # PA5-77902) using a dilution of 1:1000.

产品信息

PA5-77902

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:500-1:3,000	-
免疫组化（石蜡） (IHC (P))	1:100-1:1,000	-

产品规格
种属反应	Human, Mouse, Rat
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Recombinant protein encompassing a sequence within the N-terminus region of human CDC25A. The exact sequence is proprietary.
偶联物	Unconjugated
形式	Liquid
浓度	1 mg/mL
纯化类型	Antigen affinity chromatography
保存液	0.1M tris glycine, pH 7, with 10% glycerol
内含物	0.01% thimerosal
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2735616

产品详细信息

Positive Control: H1299, MCF-7

Predicted Reactivity: Dog (89%), Bovine (90%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

CDC25a is a member of the CDC25 family of phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. CDC25a is required for progression through G1 and S phases of the cell cycle. CDC25a activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。