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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CDC25C Antibodies

          Invitrogen

          Cdc25C Polyclonal Antibody

          View all (21) CDC25C antibodies
          Datasheet
          Protocols
          Tech Support

          Cite Cdc25C Polyclonal Antibody

          Datasheet
          Protocols
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          • Antibody Testing Data (1)
          Cdc25C Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          Cdc25C Antibody (PA5-95316) in WB

          Western blot analysis of Cdc25C in Lane 1: rat ovary tissue lysate (50 µg), Lane 2: rat liver tissue lysate (50 µg), Lane 3: HeLa whole cell lysate (40 µg), Lane 4: SW620 whole cell lysate (40 µg). Samples were incubated with Cdc25C polyclonal antibody (Product # PA5-95316). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          Product Details

          PA5-95316

          Applications
          Tested Dilution
          Publications

          免疫印迹 (WB)

          0.1-0.5 µg/mL
          -
          产品规格

          Species Reactivity

          Human, Rat

          Host/Isotype

          Rabbit / IgG

          分类

          Polyclonal

          类型

          Antibody

          Immunogen

          E.coli-derived human Cdc25C recombinant protein (Position: K175-P473).
          View immunogen

          Conjugate

          Unconjugated

          形式

          Lyophilized

          浓度

          500 µg/mL

          纯化类型

          Affinity chromatography

          保存液

          PBS with 5mg BSA

          内含物

          0.05mg sodium azide

          保存条件

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          RRID

          AB_2807119

          Product Specific Information

          Human Cdc25C shares 78.2% amino acid (aa) sequence identity with mouse Cdc25C.

          Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

          Target Information

          M-phase inducer phosphatase 3is an enzyme that in humans is encoded by theCDC25Cgene. This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 (CDK1) and triggers entry into mitosis. Also, it is thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: Dual specificity phosphatase Cdc25C; EC 3.1.3.48; M-phase inducer phosphatase 3; mitosis inducer CDC25; MPIP3; OTTHUMP00000159490; OTTHUMP00000224012; OTTHUMP00000224013; OTTHUMP00000224016; phosphotyrosine phosphatase; protein phosphatase 1, regulatory subunit 60

          View more View less

          Gene Aliases: CDC25; CDC25C; PPP1R60

          View more View less

          UniProt ID: (Human) P30307

          View more View less

          Entrez Gene ID: (Human) 995, (Rat) 307511

          View more View less

          Molecular Function: protein phosphatase

          Function(s)
          regulation of cyclin-dependent protein serine/threonine kinase activity G2/M transition of mitotic cell cycle DNA replication DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest mitotic nuclear division regulation of mitotic nuclear division spermatogenesis cell proliferation viral process peptidyl-tyrosine dephosphorylation cell division regulation of cell cycle
          Process(es)
          phosphoprotein phosphatase activity protein tyrosine phosphatase activity protein binding protein kinase binding WW domain binding
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

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