Invitrogen

Cdc42EP3 Polyclonal Antibody

Name: Cdc42EP3 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-97076
Availability: InStock

2个已发表图片

1 篇参考文献

查看其他7个CDC42EP3抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (1)
已发表图片 (2)

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Cdc42EP3 Antibody (PA5-97076) in WB

Western blot analysis of Cdc42EP3 in extracts of various cell lines. Samples were incubated with Cdc42EP3 Polyclonal antibody (Product # PA5-97076) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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Immunogen image

Immunogen Image Cdc42EP3 Polyclonal Antibody

产品信息

PA5-97076

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

查看1篇已发表文章 1篇已发表文章

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 1-254 of human CDC42EP3 (NP_0064402)

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.52 mg/mL

52 µg

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2808878

产品详细信息

Immunogen sequence: MPAKTPIYLK AANNKKGKKF KLRDILSPDM ISPPLGDFRH TIHIGKEGQH DVFGDISFLQ GNYELLPGNQ EKAHLGQFPG HNEFFRANST SDSVFTETPS PVLKNAISLP TIGGSQALML PLLSPVTFNS KQESFGPAKL PRLSCEPVME EKAQEKSSLL ENGTVHQGDT SWGSSGSASQ SSQGRDSHSS SLSEQYPDWP AEDMFDHPTP CELIKGKTKS EESLSDLTGS LLSLQLDLGP SLLDEVLNVM DKNK; Positive Samples: 293T, K-562, NIH/3T3, Rat heart; Cellular Location: Cytoplasm, Endomembrane system, Peripheral membrane protein, cytoskeleton

靶标信息

CDC42, a small Rho GTPase, regulates the formation of F-actin-containing structures through its interaction with the downstream effector proteins. The protein encoded by this gene is a member of the Borg family of CDC42 effector proteins. Borg family proteins contain a CRIB (Cdc42/Rac interactive-binding) domain. They bind to, and negatively regulate the function of, CDC42. This protein can interact with CDC42, as well as with the ras homolog gene family, member Q (ARHQ/TC10). Expression of this protein in fibroblasts has been shown to induce pseudopodia formation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。