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Cdc45L Antibody (PA5-96047) in WB
Western blot analysis of Cdc45L in extracts of various cell lines. Samples were incubated with Cdc45L Polyclonal antibody (Product # PA5-96047) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
产品信息
PA5-96047
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human CDC45 (NP_0034951)
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2807849
产品详细信息
Immunogen sequence: MFVSDFRKEF YEVVQSQRVL LFVASDVDAL CACKILQALF QCDHVQYTLV PVSGWQELET AFLEHKEQFH YFILINCGAN VDLLDILQPD EDTIFFVCDT HRPVNVVNVY NDTQIKLLIK QDDDLEVPAY EDIFRDEEED EEHSGNDSDG SEPSEKRTRL EEEIVEQTMR RRQRREWEAR RRDILFDYEQ YEYHGTSSAM VMFELAWMLS KDLNDMLWWA IVGLTDQWVQ DKITQMKYVT DVGVLQRHVS RHNHRNEDEE NTLSVDCTRI; Positive Samples: U-251MG, HeLa, A-549, HL-60, HT-29, Mouse thymus; Cellular Location: Cytoplasm, Nucleus
靶标信息
The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple polyadenylation sites of this gene are reported.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CDC 45; CDC 45L; CDC45 cell division cycle 45 homolog; CDC45 like; CDC45-related protein; Cell division control protein 45 homolog; cell division cycle 45 homolog; cell division cycle 45-like 2; human CDC45; PORC PI 1; PORC-PI-1
基因别名: CDC45; CDC45L; CDC45L2; PORC-PI-1; UNQ374/PRO710
UniProt ID: (Human) O75419, (Mouse) Q9Z1X9
Entrez Gene ID: (Human) 8318, (Rat) 287961, (Mouse) 12544
DNA replication checkpoint
G1/S transition of mitotic cell cycle
regulation of transcription involved in G1/S transition of mitotic cell cycle
double-strand break repair via break-induced replication
DNA replication
pre-replicative complex assembly involved in nuclear cell cycle DNA replication
DNA replication initiation
regulation of chromatin silencing at telomere
DNA duplex unwinding
positive regulation of G1/S transition of mitotic cell cycle
mitotic DNA replication preinitiation complex assembly
cell division
cell cycle
