Invitrogen

Citrate Synthase Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

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说明书

Protocols

技术支持

说明书

Protocols

技术支持

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Citrate Synthase Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Citrate Synthase was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Citrate Synthase Polyclonal Antibody (Product # PA5-76803) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Mitochondria localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Citrate Synthase Antibody in Western Blot (WB) — Western blot was performed using Anti-Citrate Synthase Polyclonal Antibody (Product # PA5-76803) and a 51kDa band corresponding to Citrate Synthase was observed across the cell lines tested and tissues tested. Whole cell extracts (30 µg lysate) of LNCaP (Lane 1), HeLa (Lane 2), Hep G2 (Lane 3), MCF7 (Lane 4), Mouse Brain (Lane 5), Rat Kidney (Lane 6), Rat Brain (Lane 7), Mouse Liver (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:2000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Citrate Synthase Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. Hep G2 cells were transfected with Citrate Synthase siRNA and decrease in signal intensity was observed in Western Blot application using Anti-Citrate Synthase Polyclonal Antibody (Product # PA5-76803). {KD}

产品信息

PA5-76803

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:500-1:2,000	-
免疫细胞化学 (ICC/IF)	1:50-1:200	-

产品规格
种属反应	Human, Mouse, Rat
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Recombinant full length Human Citrate Synthase.
偶联物	Unconjugated
形式	Liquid
浓度	1 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS, pH 7.2, with 50% glycerol
内含物	0.02% sodium azide
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2720530

产品详细信息

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

靶标信息

The protein encoded by this gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citrate from oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metabolism. This protein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found.

仅用于科研。不用于诊断过程。未经明确授权不得转售。