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Invitrogen
Claudin 4 Monoclonal Antibody (3E2C1)
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Claudin 4 Antibody (32-9400) in WB
Western blot was performed using Anti- Claudin 4 Monoclonal Antibody (3E2C1) (Product # 32-9400) and a 18 kDa band corresponding to CLDN4 was observed across the positive cells lines tested (MCF7 and HT-29) and not in the negative models (A549 and HeLa) as reported. Membrane enriched extracts (30 µg lysate) of MCF7 (Lane 1), HT-29 (Lane 3), A549 (Lane 2) and HeLa (Lane 4) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB230... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
32-9400
应用
建议稀释比
已发表文章
产品规格
种属反应
Dog,
Human,
Mouse,
Rat
已发表种属
Amphibian,
Bovine,
Dog,
Fish,
Human,
Mouse,
Non-human primate,
Pig,
Rabbit,
Rat,
Sheep,
Virus,
Xenopus,
Zebrafish
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
3E2C1
抗原
Synthetic peptide corresponding to a 22 amino acid sequence derived from the C-terminal region of human Claudin-4.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.5 mg/mL
规格
100 µg
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.1% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reactivity has been confirmed with human, rat ileum homogenates, and canine MDCK cell lysates by western blotting and immunofluorescence. Reactivity has also been confirmed with formalin-fixed, paraffin-embedded (FFPE) human normal colon and colon cancer tissues by immunohistochemistry.
For best results in immunohistochemistry with formalin-fixed, paraffin-embedded (FFPE) tissues, heat induced epitope retrieval (HIER) with citrate buffer, pH 6.0, is required prior to staining.
This antibody reacts specifically with the ~ 22 kDa Claudin-4 protein and does not cross-react with the protein at 55 kDa.
靶标信息
Claudin-4 belongs to the 20-member family of claudins, transmembrane cellular adhesion proteins localized at epithelial and endothelial tight junctions. Claudins demonstrate distinct, tissue-specific expression patterns, and are essential components of tight junction charge-specific channels that regulate paracellular ion flux. Claudin-4 is a component of tight junction strands and may play a role in internal organ development and function during pre- and postnatal life. The overexpression of Claudin-4 has been shown to decrease paracellular electrical conductance due to a selective decrease in Na+ permeability, with no significant decrease in Cl- permeability. In ovarian carcinoma cells, Claudin-3 and Claudin-4 exhibit upregulation at both mRNA and protein levels. Claudin-4 is also overexpressed in pancreatic cancer tissues and cell lines, and in other epithelial cancers. The Claudin-4 gene is deleted in Williams-Beuren syndrome, a neurodevelopmental disorder affecting multiple systems.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Claudin-4; CLAUDIN4; Clostridium perfringens enterotoxin receptor; CPE-R; CPE-receptor; h CPE-receptor; Williams-Beuren syndrome chromosomal region 8 protein
基因别名: Cep-r; CLDN4; CPER; Cpetr; CPETR1; WBSCR8
UniProt ID: (Dog) A0A8C0N7E5, (Mouse) O35054
Entrez Gene ID: (Dog) 100856416, (Rat) 304407, (Mouse) 12740
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Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
