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Invitrogen
Cofilin 2 Polyclonal Antibody
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Cofilin 2 Antibody (PA5-79037) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of Cofilin 2 in U2OS cells using Cofilin 2 Polyclonal Antibody (Product # PA5-79037). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL. DyLight 488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:500 di... View More
产品信息
PA5-79037
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence at the C-terminus of human Cofilin 2 (121-153aa KDAIKKKFTGIKHEWQVNGLDDIKDRSTLGEKL).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Raji whole cell lysates, human HUVEC whole cell lysates, human HepG2 whole cell lysates, human Hela whole cell lysates, rat skeletal muscle tissue lysates, rat RH35 whole cell lysates, rat liver tissue lysates, mouse skeletal muscle tissue lysates, mouse liver tissue lysates. ICC/IF: U2OS cells. Flow: Raji cells.
靶标信息
Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: COF2; cofilin 2 (muscle); Cofilin, muscle isoform; Cofilin-2; HGNC:1875; nemaline myopathy type 7
基因别名: CFL2; NEM7
UniProt ID: (Human) Q9Y281, (Mouse) P45591
Entrez Gene ID: (Human) 1073, (Rat) 366624, (Mouse) 12632
