Bioss

Complement component C9b Polyclonal Antibody

Name: Complement component C9b Polyclonal Antibody
Brand: Bioss
SKU: BS-15307R

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

Complement component C9b Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Complement component C9b Antibody (BS-15307R) in IHC (P)

Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with C9b/C9 Polyclonal Antibody, Unconjugated (bs-15307R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.

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Immunogen image

Immunogen Image Complement component C9b Polyclonal Antibody

产品信息

BS-15307R

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:300-1:1,000

免疫组化（石蜡） (IHC (P))

1:400

流式细胞分析 (Flow)

1:20-1:100

酶联免疫吸附实验 (ELISA)

1:500-1:1,000

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide derived from human Complement component C9b, amino acids 251-350.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA

内含物

0.02% ProClin 300

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

C9 is a plasma protein synthesized in the liver and monocytes consisting of a single polypeptide chain. C9 is a part of the membrane attack complex (MAC), an important component of the immune system. The MAC forms upon complement system activation by invading pathogenic bacteria and consists of the four major complement proteins: C5b, C6, C7 and C8. These complement proteins bind to the outer surface of the plasma membrane of the invading cell. C9 binds to the membrane associated C5b-8 protein, which leads to the circular polymerization of 12-18 C9 molecules. These polymerized C9 molecules form a ring structure in the membrane. Molecules can then diffuse freely through this transmembrane channel, causing cell lysis and destruction of the invading bacterial cell.

仅用于科研。不用于诊断过程。未经明确授权不得转售。