Invitrogen

Connexin 43 Monoclonal Antibody (3D8A5)

Connexin 43 Antibody in Immunohistochemistry (IHC)

图: 1 / 55

Connexin 43 Antibody (35-5000) in IHC

Double immunofluorescent staining of mouse heart atrium using mouse anti-Connexin 43 monoclonal antibody (Product # 35-5000) (red) and Rabbit anti-Connexin 40 (C-term, Product # 36-4900) (green). Cx43 immunolocalizes to the intercalated discs throughout cardiac tissue, while Cx40 immunolabeling is localized to intercalated discs restricted to the conduction system. Image courtesy of James I. Nagy, PhD, Univ... View More

Connexin 43 Antibody in Western Blot (WB)

Connexin 43 Antibody in Immunocytochemistry (ICC/IF)

Connexin 43 Antibody in Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Paraffin) (ICC/IF, IHC, IHC (P))

Connexin 43 Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))

Connexin 43 Antibody in Immunohistochemistry (Frozen) (IHC (F))

Connexin 43 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Connexin 43 Antibody in In Situ Hybridization (ISH)

Product Image Gallery

产品信息

35-5000

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

查看16篇已发表文章 16篇已发表文章

免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

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免疫组化（冰冻） (IHC (F))

Assay-dependent

查看5篇已发表文章 5篇已发表文章

免疫细胞化学 (ICC/IF)

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免疫沉淀 (IP)

查看1篇已发表文章 1篇已发表文章

In Situ Hybridization (ISH)

查看1篇已发表文章 1篇已发表文章

其他PubMed (Misc)

查看3篇已发表文章 3篇已发表文章

产品规格

种属反应

Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

3D8A5

抗原

Synthetic peptide derived from rat Connexin 43.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533207

靶标信息

Connexin 43 (Cx43) is a member of the gap junction protein family. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions. Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication. Src can interact with and phosphorylate Cx43 to alter gap junction communication. GFAP are membrane-spanning proteins that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is also targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5. Mutations in the GFAP gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy, oculodentodigital dysplasia and heart malformations. Alternatively spliced transcript variants of GFAP have been found.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in In Situ Hybridization (ISH)

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Morphological study of a connexin 43-GFP reporter mouse highlights glial heterogeneity, amacrine cells, and olfactory ensheathing cells.

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Wed Nov 01 00:00:00 UTC 2017

Immunofluorescence reveals unusual patterns of labelling for connexin43 localized to calbindin-D28K-positive interstitial cells in the pineal gland.

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Mdivi-1 induced acute changes in the angiogenic profile after ischemia-reperfusion injury in female mice.

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Effects of Ang Ⅱ perfusion on transmural heterogeneous of Cx43 in acute myocardial ischemia reperfusion.

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Wed Jan 01 00:00:00 UTC 2014

G-protein-coupled inward rectifier potassium current contributes to ventricular repolarization.

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Wed Jan 01 00:00:00 UTC 2014

Re-evaluation of connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus.

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Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Immunofluorescence

Sat Mar 01 00:00:00 UTC 2014

Re-evaluation of connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus.

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Sat Mar 01 00:00:00 UTC 2014

Re-evaluation of connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus.

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Wed Jun 15 00:00:00 UTC 2011

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Increased coupling and altered glutamate transport currents in astrocytes following kainic-acid-induced status epilepticus.

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Phosphorylation of connexin43 on serine 306 regulates electrical coupling.

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Sun Nov 01 00:00:00 UTC 2009

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Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Immunofluorescence

Wed Oct 01 00:00:00 UTC 2008

Ablation of Cx47 in transgenic mice leads to the loss of MUPP1, ZONAB and multiple connexins at oligodendrocyte-astrocyte gap junctions.

The European journal of neuroscience

Li X,Penes M,Odermatt B,Willecke K,Nagy JI

Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Immunofluorescence

Wed Oct 01 00:00:00 UTC 2008

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The European journal of neuroscience

Li X,Penes M,Odermatt B,Willecke K,Nagy JI

Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Immunofluorescence

Wed Oct 01 00:00:00 UTC 2008

Connexin mimetic peptides fail to inhibit vascular conducted calcium responses in renal arterioles.

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BMC medicine

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Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Immunofluorescence

Tue Jul 22 00:00:00 UTC 2008

Connexin-43 upregulation in micrometastases and tumor vasculature and its role in tumor cell attachment to pulmonary endothelium.

BMC medicine

Elzarrad MK,Haroon A,Willecke K,Dobrowolski R,Gillespie MN,Al-Mehdi AB

Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Immunofluorescence

Tue Jul 22 00:00:00 UTC 2008

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Neuroscience

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Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Immunofluorescence

Sun Jul 29 00:00:00 UTC 2007

Identification of connexin36 in gap junctions between neurons in rodent locus coeruleus.

Neuroscience

Rash JE,Olson CO,Davidson KG,Yasumura T,Kamasawa N,Nagy JI

Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Immunofluorescence

Sun Jul 29 00:00:00 UTC 2007

Reduction and redistribution of gap and adherens junction proteins after ischemia and reperfusion.

The Annals of thoracic surgery

Tansey EE,Kwaku KF,Hammer PE,Cowan DB,Federman M,Levitsky S,McCully JD

35-5000 was used in western blot to assess the structural changes that occur after myocardial ischemia and reperfusion

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Prolonged labour associated with lower expression of syndecan 3 and connexin 43 in human uterine tissue.

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Cluff AH,Byström B,Klimaviciute A,Dahlqvist C,Cebers G,Malmström A,Ekman-Ordeberg G

35-5000 was used in Immunohistochemistry to identify the roles of syndecan 3 and connexin 43 in the co-ordination of myometrial contractility.

Thu May 04 00:00:00 UTC 2006

Prolonged labour associated with lower expression of syndecan 3 and connexin 43 in human uterine tissue.

Reproductive biology and endocrinology : RB&E

Cluff AH,Byström B,Klimaviciute A,Dahlqvist C,Cebers G,Malmström A,Ekman-Ordeberg G

35-5000 was used in Immunohistochemistry to identify the roles of syndecan 3 and connexin 43 in the co-ordination of myometrial contractility.

Thu May 04 00:00:00 UTC 2006

Human and mouse microglia express connexin36, and functional gap junctions are formed between rodent microglia and neurons.

Journal of neuroscience research

Dobrenis K,Chang HY,Pina-Benabou MH,Woodroffe A,Lee SC,Rozental R,Spray DC,Scemes E

Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Western Blot

Tue Nov 01 00:00:00 UTC 2005

Connexin-47 and connexin-32 in gap junctions of oligodendrocyte somata, myelin sheaths, paranodal loops and Schmidt-Lanterman incisures: implications for ionic homeostasis and potassium siphoning.

Neuroscience

Kamasawa N,Sik A,Morita M,Yasumura T,Davidson KG,Nagy JI,Rash JE

Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Immunofluorescence

Tue Feb 14 00:00:00 UTC 2006

Connexin-47 and connexin-32 in gap junctions of oligodendrocyte somata, myelin sheaths, paranodal loops and Schmidt-Lanterman incisures: implications for ionic homeostasis and potassium siphoning.

Neuroscience

Kamasawa N,Sik A,Morita M,Yasumura T,Davidson KG,Nagy JI,Rash JE

Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Immunofluorescence

Tue Feb 14 00:00:00 UTC 2006

Expression of zonula occludens-1 (ZO-1) and the transcription factor ZO-1-associated nucleic acid-binding protein (ZONAB)-MsY3 in glial cells and colocalization at oligodendrocyte and astrocyte gap junctions in mouse brain.

The European journal of neuroscience

Penes MC,Li X,Nagy JI

Fri Jul 01 00:00:00 UTC 2005

The European journal of neuroscience

Penes MC,Li X,Nagy JI

Fri Jul 01 00:00:00 UTC 2005

The European journal of neuroscience

Penes MC,Li X,Nagy JI

Fri Jul 01 00:00:00 UTC 2005

Ultrastructural localization of connexins (Cx36, Cx43, Cx45), glutamate receptors and aquaporin-4 in rodent olfactory mucosa, olfactory nerve and olfactory bulb.

Journal of neurocytology

Rash JE,Davidson KG,Kamasawa N,Yasumura T,Kamasawa M,Zhang C,Michaels R,Restrepo D,Ottersen OP,Olson CO,Nagy JI

Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Immunofluorescence

Thu Sep 01 00:00:00 UTC 2005

Identification of kit positive cells in the human urinary tract.

The Journal of urology

van der AA F,Roskams T,Blyweert W,Ost D,Bogaert G,De Ridder D

35-5000 was used in immunohistochemistry - frozen section to investigate the presence of interstitial cells in the human urinary tract

Tue Jun 01 00:00:00 UTC 2004

Connexin47, connexin29 and connexin32 co-expression in oligodendrocytes and Cx47 association with zonula occludens-1 (ZO-1) in mouse brain.

Neuroscience

Li X,Ionescu AV,Lynn BD,Lu S,Kamasawa N,Morita M,Davidson KG,Yasumura T,Rash JE,Nagy JI

Tue Sep 14 00:00:00 UTC 2004

Connexin47, connexin29 and connexin32 co-expression in oligodendrocytes and Cx47 association with zonula occludens-1 (ZO-1) in mouse brain.

Neuroscience

Li X,Ionescu AV,Lynn BD,Lu S,Kamasawa N,Morita M,Davidson KG,Yasumura T,Rash JE,Nagy JI

Tue Sep 14 00:00:00 UTC 2004

Connexin47, connexin29 and connexin32 co-expression in oligodendrocytes and Cx47 association with zonula occludens-1 (ZO-1) in mouse brain.

Neuroscience

Li X,Ionescu AV,Lynn BD,Lu S,Kamasawa N,Morita M,Davidson KG,Yasumura T,Rash JE,Nagy JI

Tue Sep 14 00:00:00 UTC 2004

Coupling of astrocyte connexins Cx26, Cx30, Cx43 to oligodendrocyte Cx29, Cx32, Cx47: Implications from normal and connexin32 knockout mice.

Glia

Nagy JI,Ionescu AV,Lynn BD,Rash JE

Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Immunofluorescence

Mon Dec 01 00:00:00 UTC 2003

Coupling of astrocyte connexins Cx26, Cx30, Cx43 to oligodendrocyte Cx29, Cx32, Cx47: Implications from normal and connexin32 knockout mice.

Glia

Nagy JI,Ionescu AV,Lynn BD,Rash JE

Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Immunofluorescence

Mon Dec 01 00:00:00 UTC 2003

Connexin29 and connexin32 at oligodendrocyte and astrocyte gap junctions and in myelin of the mouse central nervous system.

The Journal of comparative neurology

Nagy JI,Ionescu AV,Lynn BD,Rash JE

Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Immunofluorescence

Mon Sep 22 00:00:00 UTC 2003

Connexin29 and connexin32 at oligodendrocyte and astrocyte gap junctions and in myelin of the mouse central nervous system.

The Journal of comparative neurology

Nagy JI,Ionescu AV,Lynn BD,Rash JE

Published figure using Connexin 43 monoclonal antibody (Product # 35-5000) in Immunofluorescence

Mon Sep 22 00:00:00 UTC 2003

生物信息学

蛋白别名: alpha 1 connexin; connexin 43; Connexin-43; Cx-43; Cx43; CXA1; DFNB38; Gap junction 43 kDa heart protein; Gap junction alpha-1 protein; gap junction membrane channel protein alpha 1; ODD; ODOD; SDTY3; Syndactyly type III

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基因别名: AU042049; AW546267; Cnx43; connexin43; Cx43; Cx43alpha1; Cxn-43; Gja-1; Gja1; Npm1

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UniProt ID: (Mouse) P23242, (Rat) P08050

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Entrez Gene ID: (Mouse) 14609, (Rat) 24392

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功能