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Invitrogen
Cre recombinase Polyclonal Antibody
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Cre recombinase Antibody (PA5-32245) in WB
Cre recombinase Polyclonal Antibody detects Cre recombinase protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg whole cell lysate/extract of Cre-ERT2-transfected 293T cells (Cre-ERT2 fusion protein size: 72 kDa).7.5% SDS-PAGE. Cre recombinase Polyclonal Antibody (Product # PA5-32245) dilution: 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
产品信息
PA5-32245
应用
建议稀释比
已发表文章
产品规格
种属反应
Virus
已发表种属
Virus
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein conjugated synthetic peptide encompassing a sequence within the center region of Cre recombinase. The exact sequence is proprietary.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Recommended positive controls: 293T transfected recombinant cre protein, IPTG-induced cell lysate from E. coli expressing Cre recombinant proteins.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
