Invitrogen

Cullin 2 Recombinant Rabbit Monoclonal Antibody (50H17L12)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2个已发表图片

3 篇参考文献

查看其他9个Cullin 2抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

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Cullin 2 Antibody (700179) in WB

Western blot analysis was performed on whole cell, tissue extracts (30 µg lysate) of HEL 92.1.7 (Lane 1), MDA-MB-231 (Lane 2), NTERA-2 (Lane 3), MCF-7 (Lane 4), A549 (Lane 5), Caco-2 (Lane 6), Mouse Testis (Lane 7), Rat Testis (Lane 8) and Mouse pancreas (Lane 9). The blots were probed with Recombinant Rabbit Monoclonal Anti-CUL2 Antibody (Product # 700179, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Recombinant Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). ... View More

Product Image Gallery

Cullin 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Cullin-2 in formalin-fixed, paraffin-embedded human lung (left) and ovarian carcimona (right) using a Cullin-2 monoclonal antibody (Product # 700179) at a dilution of 5 µg/mL. Tissues were pretreated with EDTA and staining was visualized using DAB. Images were taken at a magnification of 20x. Results show strong nuclear staining in tumor cells.

Cullin 2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of CUL-2 was performed using 70% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2 % BSA for 1 hour at room temperature. The cells were labeled with CUL-2 (50H17L12) Recombinant Rabbit Monoclonal Antibody (Product # 700179) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Cullin 2 Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of Cullin-2 in Jurkat cells using a Cullin-2 recombinant rabbit monoclonal antibody (Product # 700179) at a dilution of 0.5 µg. Cells were fixed and permeabilized using FIX & PERM (Product # GAS-004) reagent, and detection was performed using an Alexa Fluor 488 goat anti-rabbit IgG (black) compared to a control without primary antibody (gray). Pre-incubation with the immunogenic peptide decreased the signal (red).

Cullin 2 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. NTERA-2 cl.D1 cells were transfected with Cullin 2 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-Cullin 2 Recombinant Rabbit Monoclonal Antibody (50H17L12) (Product # 700179). {KD}

产品信息

700179

应用

建议稀释比

已发表文章

免疫印迹 (WB)

3-5 µg/mL

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免疫组化（石蜡） (IHC (P))

4-6 µg/mL

免疫细胞化学 (ICC/IF)

8-12 µg/mL

流式细胞分析 (Flow)

0.5-1 µg/test

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

50H17L12

抗原

A peptide corresponding to amino acids 733-745 of Q13617.

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偶联物

Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2532291

产品详细信息

This antibody is predicted to react with bovine, canine, chimpanzee, equine, orangutan, primate, Rhesus monkey and Xenopus based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. ECS complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (PubMed:27565346). May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF). [UniProt]

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Unanchored tri-NEDD8 inhibits PARP-1 to protect from oxidative stress-induced cell death.

The EMBO journal

Keuss MJ,Hjerpe R,Hsia O,Gourlay R,Burchmore R,Trost M,Kurz T

700179 was used in Western Blotting to show that trimeric, acetylated NEDD8 attenuates PARP-1 activation after oxidative stress, likely to delay the initiation of PARP-1-dependent cell death.

Fri Mar 15 00:00:00 EDT 2019

Differential PROTAC substrate specificity dictated by orientation of recruited E3 ligase.

Nature communications

Smith BE,Wang SL,Jaime-Figueroa S,Harbin A,Wang J,Hamman BD,Crews CM

700179 was used in Western Blotting to generate isoform-selective PROteolysis-TArgeting Chimeras (PROTACs) for the p38 MAPK family using a single warhead and recruited E3 ligase.