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Cyt b6/PetB/Thylakoid membrane cytochrome b6 protein Polyclonal Antibody
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Cyt b6/PetB/Thylakoid membrane cytochrome b6 protein Antibody (AS18-4169) in WB
0.75 µg of chlorophyll from Pisum sativum thylakoids and from mesophyll (M) and bundle sheath (BS) thylakoids of Zea mays, Panicum miliaceum, Echinochloa crus-galli extracted with 0.4 M sorbitol, 50 m M Hepes Na OH, p H 7.8,10 m M Na Cl, 5 m M Mg Cl 2 and 2 m M EDTA were loaded to lanes. Samples were denatured with LaemmLi buffer at 75 °C for 5 min and were separated on 12% SDS-PAGE and blotted 30 min to PV... View More
产品信息
AS18-4169
产品规格
种属反应
Algae,
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide chosen from Arabidopsis thaliana PetB protein sequence.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Echinochloa crus-galli, Marchantia polymorpha, Medicago sativa, Nannochloropsis oceanica strain IMET1, Nicotiana benthamiana, Panicum miliaceum, Pisum sativum, Zea mays
靶标信息
petB (cytochrome b6; Cyt b6) is a plastid-encoded integral membrane protein that accumulates in the thylakoid membrane as a core catalytic subunit of the cytochrome b6f complex, positioned between photosystem II and photosystem I. The PetB polypeptide is highly hydrophobic (typically ~215-220 aa) and forms the N-terminal half of the cytochrome b6 fold with four transmembrane helices (often described as helices A-D), providing conserved heme-binding motifs that coordinate two b-type hemes and contributing to formation of the quinone reaction space together with other b6f subunits. Functionally, PetB supports plastoquinol:plastocyanin oxidoreductase activity by enabling Q-cycle electron bifurcation at the Qo/Qi sites, coupling electron transfer from plastoquinol to downstream carriers (via the Rieske 2Fe-2S protein and cytochrome f) with vectorial proton translocation across the thylakoid membrane to build the proton-motive force that drives ATP synthesis; cytochrome b6f thereby sets a key control point for linear electron flow and also participates in cyclic electron flow and regulatory signaling linked to light acclimation and state transitions.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: cyt b6; cytochrome b6; petB
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