Invitrogen

Cytochrome C Monoclonal Antibody (2B5), eBioscience™

Cytochrome C Antibody in Immunocytochemistry (ICC/IF)

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Cytochrome C Antibody (BMS1037) in ICC/IF

Immunofluorescence analysis of Cytochrome C was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Cytochrome C Monoclonal Antibody (2B5), eBioscience™ (Product # BMS1037) at 1:100 dilution in 0.1% BSA, ... View More

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产品信息

BMS1037

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-Dependent

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:100

流式细胞分析 (Flow)

Assay-Dependent

酶联免疫吸附实验 (ELISA)

Assay-Dependent

查看2篇已发表文章 2篇已发表文章

免疫沉淀 (IP)

Assay-Dependent

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

2B5

抗原

Rec. Human Cytochrome C

偶联物

Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2-7.4

内含物

no preservative

保存条件

4° C

运输条件

Wet ice

RRID

AB_10598651

产品详细信息

Description: Cytochrome c was identified as a component required for the crucial steps in apoptosis, caspase-3 activation and DNA fragmentation. Cytochrome c was shown to redistribute from mitochondria to cytosol during apoptosis in intact cells.

Mitochrondrial cytochrome c is a water-soluble protein of 15 kDa with a net positive charge, residing loosely attached in the mitochrondrial intermembrane space. Cytochrome c functions in the respiratory chain by interaction with redox partners. The release of cytochrome c into the cytosol leads to an activation of an apoptotic program via activation of a caspase dependent pathway. Cytochrome c achieves this goal by interaction with other cytosolic factors forming a complex (apoptosome) composed of cytochrome c, Apaf-1, dATP and Apaf-3/caspase 9. Bcl-2 on the other hand was shown to be able to prevent apoptosis by blocking the release of cytochrome c from mitochondria.

Applications Tested: ELISA, Flow Cytometry, Western Blotting.

靶标信息

Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

GSK3β Inhibition Is the Molecular Pivot That Underlies the Mir-210-Induced Attenuation of Intrinsic Apoptosis Cascade during Hypoxia.

International journal of molecular sciences

Marwarha G,Røsand Ø,Slagsvold KH,Høydal MA

BMS1037 was used in Enzyme-linked immunosorbent assay to unveil that the inhibition of GSK3β kinase activity is indispensable in mediating the miR-210-orchestrated protective cellular response to hypoxia-induced apoptotic cell death.

Fri Aug 19 00:00:00 EDT 2022

miR-210 Regulates Apoptotic Cell Death during Cellular Hypoxia and Reoxygenation in a Diametrically Opposite Manner.

Biomedicines

Marwarha G,Røsand Ø,Scrimgeour N,Slagsvold KH,Høydal MA

BMS1037 was used in Western Blot, ELISA to investigate the polarity of the miR-210-elicited cellular response, as miR-210 has been shown to exacerbate as well as attenuate hypoxia-driven apoptotic cell death.