Invitrogen

Cytochrome C Monoclonal Antibody (6H2), FITC, eBioscience™

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

3个已发表图片

4 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

Product Image Gallery

Cytochrome C Antibody in Flow Cytometry (Flow) — Intracellular staining of the HeLa cell line with anti-Cytochrome c FITC. Appropriate isotype controls were used (open histogram). Total cells were used for analysis.

Cytochrome C Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Cytochrome C was performed using log phase HeLa cells treated with 50 uM of Etoposide for 3 hrs. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cytochrome C, FITC conjugated Monoclonal Antibody (Product # 11-6601-82) at 5µg/mL in 0.1% BSA and incubated overnight at 4 degree (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). Mitochondria was stained with MitoTracker® Red CMXRos (Product # M-7512). Panel d represents the merged image showing cytoplasmic release of Cytochrome C from mitochondria on etoposide treatment. Panel e represents untreated cells showing mitochondrial localization. Panel f represents FITC Isotype control cells to assess background. The images were captured at 60X magnification.

Cytochrome C Antibody in Immunohistochemistry (IHC) — Figure 5 Demonstration of Cytochrome-c-CED-4 interaction in S.digitata and docking of Cytochrome-c and CED-4 of C.elegans. [ A ] Confocal images of untreated control [upper panel] or Plumbagin treated [lower panel] late embryonic stages demonstrating enhanced colocalization of Cytochrome-c and CED-4 after 24 hr Plumbagin treatment are shown. Regions of colocalization are highlighted as white patches using Image J software. Fluorescence intensity line profile/colocalization profile analysis for both the labeled proteins-Cytochrome-c and CED-4 in control as well as apoptotic embryos, revealing enhanced cololocalization of these proteins in the later are shown [ B ] Interaction of Cytochrome-c of C.elegans [ Magenta ] with alpha/beta [P-loop] ATP binding domain [ Blue ] of CED-4 is shown.

Cytochrome C Antibody — Detection of altered subcellular localization of the target protein by cell treatment demonstrates antibody specificity. Immunofluorescence analysis of Cytochrome C using Cytochrome C FITC conjugated Monoclonal antibody (Product # 11-6601-82), shows change in localization of Cytochrome C from mitochondria to cytoplasm in Hela cells upon etoposide treatment.

产品信息

11-6601-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

5 µg/mL

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流式细胞分析 (Flow)

1 µg/test

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

6H2

偶联物

FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

RRID

AB_465365

产品详细信息

Description: The 6H2 antibody reacts with the native form of mouse, human, and rat cytochrome c.

Applications Reported: The 6H2 antibody has been reported for use in intracellular flow cytometric analysis.

Applications Tested: The 6H2 antibody has been tested by intracellular flow cytometric analysis. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Inhibition of antiapoptotic BCL-2 proteins with ABT-263 induces fibroblast apoptosis, reversing persistent pulmonary fibrosis.

JCI insight

Cooley JC,Javkhlan N,Wilson JA,Foster DG,Edelman BL,Ortiz LA,Schwartz DA,Riches DW,Redente EF

11-6601-82 was used in Flow cytometry/Cell sorting to conclude that, by targeting profibrotic fibroblasts, ABT-263 offers a promising therapeutic option for progressive fibrosing interstitial lung diseases (PF-ILDs).