Invitrogen

Cytochrome C Monoclonal Antibody (6H2), FITC, eBioscience™

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1个已发表图片

1篇引用文献

查看其他33个Cytochrome C抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

Product Image Gallery

Cytochrome C Antibody in Flow Cytometry (Flow) — Intracellular staining of the HeLa cell line with anti-Cytochrome c FITC. Appropriate isotype controls were used (open histogram). Total cells were used for analysis.

Cytochrome C Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Cytochrome C was performed using log phase HeLa cells treated with 50 uM of Etoposide for 3 hrs. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cytochrome C, FITC conjugated Monoclonal Antibody (Product # 11-6601-82) at 5µg/mL in 0.1% BSA and incubated overnight at 4 degree (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). Mitochondria was stained with MitoTracker® Red CMXRos (Product # M7512). Panel d represents the merged image showing cytoplasmic release of Cytochrome C from mitochondria on etoposide treatment. Panel e represents untreated cells showing mitochondrial localization. Panel f represents FITC Isotype control cells to assess background. The images were captured at 60X magnification.

Cytochrome C Antibody — Detection of altered subcellular localization of the target protein by cell treatment demonstrates antibody specificity. Immunofluorescence analysis of Cytochrome C using Cytochrome C FITC conjugated Monoclonal antibody (Product # 11-6601-82), shows change in localization of Cytochrome C from mitochondria to cytoplasm in Hela cells upon etoposide treatment. {TM}

Cytochrome C Antibody in Immunohistochemistry (IHC) — Figure 5 Demonstration of Cytochrome-c-CED-4 interaction in S.digitata and docking of Cytochrome-c and CED-4 of C.elegans. [ A ] Confocal images of untreated control [upper panel] or Plumbagin treated [lower panel] late embryonic stages demonstrating enhanced colocalization of Cytochrome-c and CED-4 after 24 hr Plumbagin treatment are shown. Regions of colocalization are highlighted as white patches using Image J software. Fluorescence intensity line profile/colocalization profile analysis for both the labeled proteins-Cytochrome-c and CED-4 in control as well as apoptotic embryos, revealing enhanced cololocalization of these proteins in the later are shown [ B ] Interaction of Cytochrome-c of C.elegans [ Magenta ] with alpha/beta [P-loop] ATP binding domain [ Blue ] of CED-4 is shown.

产品信息

11-6601-82

应用	建议稀释比	已发表文章
免疫组化 (IHC)	-	查看1篇已发表文章 1篇已发表文章
免疫细胞化学 (ICC/IF)	5 µg/mL	-
流式细胞分析 (Flow)	1 µg/test	-

产品规格
种属反应	Human, Mouse, Rat
已发表种属	Not Applicable
宿主/亚型	Mouse / IgG1, kappa
推荐的同型对照	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™
分类	Monoclonal
类型	Antibody
克隆号	6H2
偶联物	FITC Unconjugated
激发/发射光谱	查看光谱
形式	Liquid
浓度	0.5 mg/mL
纯化类型	Affinity chromatography
保存液	PBS, pH 7.2
内含物	0.09% sodium azide
保存条件	4° C, store in dark, DO NOT FREEZE!
RRID	AB_465365

产品详细信息

Description: The 6H2 antibody reacts with the native form of mouse, human, and rat cytochrome c.

Applications Reported: The 6H2 antibody has been reported for use in intracellular flow cytometric analysis.

Applications Tested: The 6H2 antibody has been tested by intracellular flow cytometric analysis. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Flow Cytometry

Panel Builder