Invitrogen

Cytochrome C Monoclonal Antibody (7H8.2C12)

Name: Cytochrome C Monoclonal Antibody (7H8.2C12)
Brand: Invitrogen
SKU: MA5-11674

高级验证

7个已发表图片

42 篇参考文献

查看其他50个Cytochrome C抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

Cytochrome C Antibody in Immunocytochemistry (ICC/IF)

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Cytochrome C Antibody (MA5-11674) in ICC/IF

Immunofluorescence analysis of Cytochrome was performed using 70 % confluent log phase HeLa cells. The cells were fixed with 4 % paraformaldehyde for 10 minutes, permeabilized with 0.1 % Triton™ X-100 for 15 minutes, and blocked with 2 % BSA for 45 minutes at room temperature. The cells were labeled with Cytochrome C Monoclonal Antibody (7H8.2C12) (Product # MA5-11674) at 0.66 µg/mL in 0.1 % BSA, incubated ... View More

Cytochrome C Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Cytochrome C Antibody in Immunohistochemistry (IHC)

Cytochrome C Antibody in Western Blot (WB)

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产品信息

MA5-11674

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

查看21篇已发表文章 21篇已发表文章

免疫组化 (IHC)

查看8篇已发表文章 8篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100

查看5篇已发表文章 5篇已发表文章

免疫细胞化学 (ICC/IF)

2 µg/mL

查看4篇已发表文章 4篇已发表文章

其他PubMed (Misc)

查看4篇已发表文章 4篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

C. elegans, Fruit fly, Human, Mouse, Rat, Xenopus

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

7H8.2C12

抗原

Synthetic peptides corresponding to amino acids 1-80, 81-104 and 66-104 of pigeon cytochrome c

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Protein A/G

保存液

PBS, pH 7.4, with 0.05% BSA

内含物

0.05% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_10985701

产品详细信息

MA5-11674 targets Cytochrome c in IHC (P) and WB applications and shows reactivity with Human, mouse, and Rat samples.

The MA5-11674 immunogen is synthetic peptides corresponding to amino acids 1-80, 81-104 and 66-104 of pigeon cytochrome c.

靶标信息

Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。