Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is prostate cancer or skin tissue.
Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyperproliferation-related keratins). Keratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyperproliferative situations. Epidermal injury results in activation of keratinocytes which express CK6 and CK16. CK6 is strongly expressed in about 75% of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation.
蛋白别名: 60-kDa keratin; CK-6A; CK-6B; CK-6C; CK-6D; CK-6E; cytokeratin 6A; cytokeratin 6B; cytokeratin 6C; cytokeratin 6D; Cytokeratin-6A; Cytokeratin-6B; Cytokeratin-6C; Cytokeratin-6D; Cytokeratin-6E; Hom s 5; K6A; K6a keratin; K6B; K6C; kamp-keratin derived antimicrobial peptide; KDAMP; keratin 6A, , type II; keratin 6A, type II; keratin 6B, type II; keratin 6C, type II; keratin 6E; keratin complex 2, basic, gene 6a; keratin complex 2, basic, gene 6b; keratin complex 2, basic, gene 6c; keratin complex 2, gene 6a; keratin K6; Keratin K6h; keratin, epidermal type II, K6A; keratin, epidermal, type II, K6B; Keratin, type II cytoskeletal 6A; Keratin, type II cytoskeletal 6B; Keratin, type II cytoskeletal 6C; Keratin-6-alpha; keratin-6-beta; Keratin-6A; Keratin-6B; Keratin-6C; keratin-like 1 (a type II keratin sequence); mK6-alpha; mK6-beta; Type-II keratin Kb10; Type-II keratin Kb12; Type-II keratin Kb6
基因别名: 2310016L08Rik; BB144589; CK-6A; CK-6B; CK-6C; CK-6E; CK6A; CK6B; CK6C; CK6D; K6A; K6B; K6C; K6D; K6E; Ker2; Krt2-6; Krt2-6a; Krt2-6b; Krt2-6c; Krt6; KRT6A; KRT6B; KRT6C; KRT6D; KRT6E; KRTL1; mK6[a]; mK6[b]; MK6a; PC2; PC3; PC4; PPKNEFD