Invitrogen

Cytokeratin 8 Monoclonal Antibody (LP3K), eBioscience™

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

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Cytokeratin 8 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Cytokeratin 8 was performed using 70% confluent log phase Caco-2 cells. The cells were fixed with ice-cold acetone at 4°C for 5 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Cytokeratin 8 Monoclonal Antibody (LP3K), eBioscience™ (Product # 14-9938-82) at a concentration of 1 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing cytoskeletal localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Cytokeratin 8 Antibody in Western Blot (WB) — CRISPR-Cas9 mediated genome editing ofCytokeratin 8 (as confirmed by next generation sequencing) was achieved by using LentiArray™ Lentiviral sgRNA (Product # A32042, AssayID CRISPR745510_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Fig (a) Western blot analysis of Cytokeratin 8 was performed by loading 30 µg of HeLa Wild Type (Lane 1), HeLa Cas9 (Lane 2) and HeLa Cas9 cells transduced with Cytokeratin 8 Lentiviral sgRNA (Lane 3) whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with Anti-Cytokeratin 8 Monoclonal Antibody (LP3K), eBioscience™ (Product # 14-9938-82) using 1 µg/mL dilution and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177 1:5,000 dilution).Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). A loss of signal in sgRNA transduced cells using the LentiArray™ CRISPR product line confirms that antibody is specific toCytokeratin 8 (Fig (b)).

Cytokeratin 8 Antibody in Immunohistochemistry (IHC) — Figure 7. SeqStain multiplex imaged panels identify major substructures in the human kidney (A) Image showing a composite overlay of aligned immunofluorescence image stack of 20 unique markers from the whole kidney tissue section. Scale bar, 100 mum. (B) Image of a zoomed-in region from the whole kidney tissue section in (A) (white square) showing various components of the kidney tissue, including the glomerular endothelial cells (GECs), proximal tubule (PT), collecting duct (CD), distal convoluted tubules (DCT), and podocytes. Scale bar, 100 mum. (C) Boxed dot plot showing computed co-expression of EpCAM and the indicated markers on a per-cell basis (mean +- SD). (D) Image of a zoomed-in region from the whole kidney tissue section in (A) (red square) showing one glomerulus (two panels), false colored for the indicated markers. Scale bar, 100 mum. (E) Representative images showing zoomed-in regions of composite overlay of aligned immunofluorescence image stacks from (A) for the identification of various immunophenotypes of cells and tissue sections based on co-localization of various markers (as labeled). Scale bar, 50 mum. (F) Boxed dot plot of cellular neighborhoods showing the computed distances of the indicated cells from glomerular basement membrane of a selected glomerulus in the kidney (mean +- SD). Glom refers to cells residing inside the glomerulus, whereas RI refers to cells residing outside the glomerulus, in the renal interstitium.

Cytokeratin 8 Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein in HeLa Cas9 cell line transduced with Cytokeratin 8 Lentiviral sgRNA compared to control cell line using Anti-Cytokeratin 8 Monoclonal Antibody (LP3K), eBioscience™ (Product # 14-9938-82). {KO}

产品信息

14-9938-82

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1-10 µg/mL	-
免疫组化 (IHC)	-	查看1篇已发表文章 1篇已发表文章
免疫细胞化学 (ICC/IF)	1-10 µg/mL	-

产品规格
种属反应	Human
已发表种属	Not Applicable
宿主/亚型	Mouse / IgG1
分类	Monoclonal
类型	Antibody
克隆号	LP3K
偶联物	Unconjugated FITC
形式	Liquid
浓度	0.5 mg/mL
纯化类型	Affinity chromatography
保存液	PBS, pH 7.2
内含物	0.09% sodium azide
保存条件	4° C
RRID	AB_10557242

产品详细信息

Description: This LP3K monoclonal antibody reacts with human cytokeratin 8 (K8), a 55-kDa member of the family of intermediate filament proteins. Cytokeratin 8 is a type II (or basic) keratin that is expressed in epithelial and carcinoma cells. Cytokeratins form the intracellular cytoskeletal network that maintains the integrity and stability of cells and tissues. More specifically, studies have demonstrated the involvement of cytokeratin 8 in protection against apoptosis, stress, or injury, as well as regulation of the cell cycle. This keratin is frequently co-expressed with cytokeratin 18, a type I (or acidic) keratin as a heterodimer. Although detected primarily in the cytoplasm of normal healthy cells, cytokeratin 8 has been found to localize to the plasma membrane in some tumor cells. Finally, cytokeratin 8 is phosphorylated on serine 73 in dividing cells.

Applications Reported: This LP3K antibody has been reported for use in immunoblotting (WB) and immunocytochemistry.

Applications Tested: This LP3K antibody has been tested by western blot analysis of reduced A549 cell lysate, as well as by immunocytochemistry of A549 cells. For western blotting, this antibody can be used at 1-10 µg/mL. For immunocytochemistry, LP3K can be used at 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Cytokeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. Cutokeratin 8 typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

SeqStain is an efficient method for multiplexed, spatialomic profiling of human and murine tissues.

Cell reports methods

Rajagopalan A,Venkatesh I,Aslam R,Kirchenbuechler D,Khanna S,Cimbaluk D,Kordower JH,Gupta V

Published figure using Cytokeratin 8 monoclonal antibody (Product # 14-9938-82) in Immunocytochemistry

Mon Jun 21 00:00:00 EDT 2021

生物信息学

蛋白别名: C CY K2C8; CK 8; CK-8; Cytokeratin-8; hard keratin; K-8; K8; keratin 8, type II; Keratin, type II cytoskeletal 8; Keratin-8; Type-II keratin Kb8

基因别名: CARD2; CK-8; CK8; CYK8; K2C8; K8; KO; KRT8

UniProt ID: (Human) P05787

Entrez Gene ID: (Human) 3856

功能

viral process tumor necrosis factor-mediated signaling pathway sarcomere organization response to hydrostatic pressure response to other organism cell differentiation involved in embryonic placenta development extrinsic apoptotic signaling pathway hepatocyte apoptotic process

参与通路

structural molecule activity protein binding protein complex binding scaffold protein binding

Cytokeratin 8 Monoclonal Antibody (LP3K), eBioscience™

产品信息

14-9938-82

免疫印迹 (WB)

免疫组化 (IHC)

免疫细胞化学 (ICC/IF)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

生物信息学

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