Invitrogen

DAPK1 Recombinant Rabbit Monoclonal Antibody (7H26L19)

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DAPK1 Antibody (702862) in WB

Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of SH-SY5Y (Lane 1), MKN45 (Lane 2), MDA-MB-231 (Lane 3), MCF-7 (Lane 4) and tissue extracts of Mouse Brain (Lane 5). The blot was probed with Anti-DAPK1 Recombinant Rabbit Monoclonal Antibody (Product # 702862, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:5000 dilution). A ~160 kDa band corresponding to DAPK1 was observed in relevant cell lines a... View More

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DAPK1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target between cell models owing to their inherent genetic constitution. Higher expression of DAPK1 protein was observed in SH-SY5Y, MKN45 and Mouse Brain in comparison to MDA-MB-231 and MCF-7 using Anti-DAPK1 Recombinant Rabbit Monoclonal Antibody (Product # 702862, 1:1000 dilution) in Western blot (PMID: 26075823). {RE}

产品信息

702862

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

7H26L19

抗原

Protein corresponding to human DAPK1 [aa681-aa955]

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2762414

产品详细信息

This antibody is predicted to react with Monkey, Pig, Cat.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

DAPK1 is a large, calmodulin-dependent kinase involved in gamma-interferon induced programmed cell death and is a potential tumor suppressor. DAPK1 is involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and authophagy. It regulates both type I apoptotic and type II autophagic cell death signal, depending on the cellular setting. Type I apoptotic cell death is caspase-dependent, type II authophagic cell death is caspase-independent. DAPK1 phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. It also phosphorylates TPM1, STX1A, PRKD1, BECN1, TSC2, RPS6, MYL9, and DAPK3.

仅用于科研。不用于诊断过程。未经明确授权不得转售。