Invitrogen

DCI Polyclonal Antibody

Product Image Gallery

DCI Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of DCI using anti-DCI antibody (Product # PA5-79184) . DCI was detected in a section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-DCI antibody (Product # PA5-79184) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

DCI Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of DCI on paraffin-embedded human mammary cancer tissue. Sample was incubated with DCI polyclonal antibody (Product# PA5-79184).

DCI Antibody in Western Blot (WB) — Western blot analysis of DCI in Lane 1: rat liver tissue lysate, Lane 2: human placenta tissue lysate, Lane 3: A549 whole cell lysate, Lane 4: SMMC whole cell lysate, Lane 5: COLO320 whole cell lysate, Lane 6: HeLa whole cell lysate, Lane 7: HT1080 whole cell lysate using 40-50 µg per well. Sample was incubated with DCI (Product # PA5-79184) at a dilution of 0.5 µg/mL.

产品信息

PA5-79184

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

2 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence at the C-terminus of human DCI (272-290aa ADVQNFVSFISKDSIQKSL).

查看免疫原

偶联物

Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5mg BSA

内含物

0.05mg sodium azide, 0.05mg thimerosal

保存条件

-20°C

RRID

AB_2746300

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

靶标信息

Protein names: Enoyl-CoA delta isomerase 1, mitochondrial; 3,2-trans-enoyl-CoA isomerase; Delta(3),Delta(2)-enoyl-CoA isomerase; Short name=D3,D2-enoyl-CoA isomerase; Dodecenoyl-CoA isomerase. Gene names: ECI1, DCI. Function: Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Catalytic activity: (3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA. Pathway: Lipid metabolism; fatty acid beta-oxidation. Subunit structure: Homotrimer. Subcellular location: Mitochondrion matrix.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: 3,2 trans-enoyl-Coenyme A isomerase; 3,2 trans-enoyl-Coenzyme A isomerase; 3,2-trans-enoyl-CoA isomerase; 3,2-trans-enoyl-CoA isomerase, mitochondrial; ACBD2; acetylene-allene isomerase; D3,D2-enoyl-CoA isomerase; Delta(3),Delta(2)-enoyl-CoA isomerase; dodecenoyl-CoA de; dodecenoyl-CoA delta isomerase (3,2 trans-enoyl-CoA isomerase); Dodecenoyl-CoA isomerase; dodecenoyl-Coenzyme A delta isomerase (3,2 trans-enoyl-Coenyme A isomerase); dodecenoyl-Coenzyme A delta isomerase (3,2 trans-enoyl-Coenzyme A isomerase); DRS 1; DRS1; Enoyl-CoA delta isomerase 1, mitochondrial; enoyl-Coenzyme A delta isomerase 1; HCA88; Peroxisomal 3

基因别名: DCI; eci; ECI1

UniProt ID: (Human) P42126, (Mouse) P42125

Entrez Gene ID: (Human) 1632, (Rat) 29740, (Mouse) 13177

分子生物学功能: hydratase