Invitrogen

DDR2 Polyclonal Antibody

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DDR2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of DDR2 in paraffin-embedded mouse small intestine tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with DDR2 polyclonal antibody (Product # PA5-95551) at a 2 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex and DAB.

DDR2 Antibody in Western Blot (WB) — Western blot analysis of DDR2 in Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with DDR2 polyclonal antibody (Product # PA5-95551) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL).

产品信息

PA5-95551

应用	建议稀释比	已发表文章
免疫印迹 (WB)	0.1-0.5 µg/mL	-
免疫组化（石蜡） (IHC (P))	0.5-1 µg/mL	-
酶联免疫吸附实验 (ELISA)	0.1-0.5 µg/mL	-

产品规格
种属反应	Human, Mouse, Rat
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	E.coli-derived human DDR2 recombinant protein (Position: T801-E855).
偶联物	Unconjugated
形式	Lyophilized
浓度	500 µg/mL
纯化类型	Affinity chromatography
保存液	PBS with 4mg trehalose
内含物	0.05mg sodium azide
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2807353

产品详细信息

Reconstitution information: Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

靶标信息

Tyrosine kinase that functions as cell surface receptor for fibrillar collagen and regulates cell differentiation, remodeling of the extracellular matrix, cell migration and cell proliferation. Required for normal bone development. Regulates osteoblast differentiation and chondrocyte maturation via a signaling pathway that involves MAP kinases and leads to the activation of the transcription factor RUNX2. Regulates remodeling of the extracellular matrix by up-regulation of the collagenases MMP1, MMP2 and MMP13, and thereby facilitates cell migration and tumor cell invasion. Promotes fibroblast migration and proliferation, and thereby contributes to cutaneous wound healing.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: CD167 antigen-like family member B; CD167b; CD167b antigen; CD_antigen: CD167b; cell migration-inducing protein 20; Discoidin domain receptor 2; discoidin domain receptor family, member 2; Discoidin domain-containing receptor 2; Discoidin domain-containing receptor tyrosine kinase 2; hydroxyaryl-protein; hydroxyaryl-protein kinase; migration-inducing gene 16 protein; neurotrophic tyrosine kinase receptor related 3; Neurotrophic tyrosine kinase, receptor-related 3; Receptor protein-tyrosine kinase TKT; RP11-572K18.1; tyrosine-protein kinase TYRO 10; Tyrosine-protein kinase TYRO10; tyrosylprotein kinase

基因别名: AW495251; DDR2; MIG20a; NTRKR3; TKT; TYRO10

UniProt ID: (Human) Q16832, (Mouse) Q62371

Entrez Gene ID: (Human) 4921, (Mouse) 18214, (Rat) 685781

分子生物学功能: transmembrane signal receptor