Invitrogen

DDR2 Polyclonal Antibody

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DDR2 Antibody (PA5-95551) in WB

Western blot was performed using Anti-DDR2 Polyclonal Antibody (Product # PA5-95551) and a 130 kDa band corresponding to Ddr2 was observed in IMR-32, SH-SY5Y, HT-1080 and BJ, which are reported to be positive cell models for Ddr2 but not in MCF7 and SK-OV-3, which are negative cell models. Membrane enriched extracts (30 µg lysate) of IMR-32 (Lane 1), SH-SY5Y (Lane 2), HT-1080 (Lane 3), BJ (Lane 4), MCF7 (Lane 5) and SK-OV-3 (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were ... View More

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DDR2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of DDR2 in paraffin-embedded mouse small intestine tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with DDR2 polyclonal antibody (Product # PA5-95551) at a 2 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex and DAB.

产品信息

PA5-95551

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

酶联免疫吸附实验 (ELISA)

0.1-0.5 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human DDR2 recombinant protein (Position: T801-E855).

查看免疫原

偶联物

Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2807353

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

靶标信息

DDR2 is a member of a subclass of RTKs and contains a distinct extracellular region encompassing a factor VIII-like domain. DDR1 and DDR2 have been shown to be potently inhibited by Gleevec. DDR2 required for normal bone development. Regulates osteoblast differentiation and chondrocyte maturation via a signaling pathway that involves MAP kinases and leads to the activation of the transcription factor RUNX2. Regulates remodeling of the extracellular matrix by up-regulation of the collagenases MMP1, MMP2 and MMP13, and thereby facilitates cell migration and tumor cell invasion. Promotes fibroblast migration and proliferation, and thereby contributes to cutaneous wound healing.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: CD167 antigen-like family member B; CD167b; CD167b antigen; CD_antigen: CD167b; cell migration-inducing protein 20; Discoidin domain receptor 2; discoidin domain receptor family, member 2; Discoidin domain-containing receptor 2; Discoidin domain-containing receptor tyrosine kinase 2; hydroxyaryl-protein; hydroxyaryl-protein kinase; migration-inducing gene 16 protein; neurotrophic tyrosine kinase receptor related 3; Neurotrophic tyrosine kinase, receptor-related 3; Receptor protein-tyrosine kinase TKT; RP11-572K18.1; tyrosine-protein kinase TYRO 10; Tyrosine-protein kinase TYRO10; tyrosylprotein kinase

基因别名: AW495251; DDR2; MIG20a; NTRKR3; TKT; TYRO10

UniProt ID: (Human) Q16832, (Mouse) Q62371

Entrez Gene ID: (Human) 4921, (Mouse) 18214, (Rat) 685781

分子生物学功能: transmembrane signal receptor