Invitrogen

DDX55 Polyclonal Antibody

查看其他7个DDX55抗体

问题与答案

问题与答案

问题与答案

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抗体检测数据 (2)

DDX55 Antibody in Immunocytochemistry (ICC/IF)

DDX55 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 2

DDX55 Antibody (PA5-115108) in ICC/IF

Immunocytochemistry analysis of DDX55 in HepG2 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115108). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.

DDX55 Antibody in Immunocytochemistry (ICC/IF)

DDX55 Antibody in Western Blot (WB)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

DDX55 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of DDX55 in HepG2 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115108). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.

DDX55 Antibody in Western Blot (WB) — Western blot analysis of DDX55 in A375 cells (left lane: treated with blocking peptide). Samples were incubated with polyclonal antibody (Product # PA5-115108).

Immunogen image

DDX55 Polyclonal Antibody

Immunogen Image DDX55 Polyclonal Antibody

产品信息

PA5-115108

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human DDX55(Accession Q8NHQ9), corresponding to amino acid residues V101-R151.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody detects endogenous levels of total DDX55.

靶标信息

This gene encodes an enzyme which ubiquitinates proteins which participate in signaling pathways and apoptosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: ATP-dependent RNA helicase DDX55; DEAD box protein 55

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基因别名: 2810021H22Rik; DDX55; KIAA1595; mKIAA1595

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UniProt ID: (Mouse) Q6ZPL9

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Entrez Gene ID: (Mouse) 67848

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nucleotide binding nucleic acid binding RNA binding ATP-dependent RNA helicase activity helicase activity ATP binding hydrolase activity poly(A) RNA binding

biological_process RNA secondary structure unwinding