Invitrogen

DNMT3B Polyclonal Antibody

DNMT3B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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DNMT3B Antibody (PA1-884) in IHC (P)

Immunohistochemistry analysis of DNA Methyltransferase 3 showing positive staining in the nucleus and cytoplasm of paraffin-treated Human breast carcinoma (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blo... View More

DNMT3B Antibody in Immunohistochemistry (IHC)

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产品信息

PA1-884

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2.0 µg/mL

查看10篇已发表文章 10篇已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to the residues M(1) K G D S R H L N E E E G A(14) C of mouse DNMT3b

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2277463

产品详细信息

PA1-884 detects DNA methyltransferase 3 from human and mouse tissues and cells as well as recombinant human Dnmt3. This antibody has been used to detect both recombinant and endogenous DNMT3b and recombinant DNMT3a.

PA1-884 has been successfully used in Immunohistochemistry (paraffin) and Western blot procedures. By Western blot, this antibody detects DNMT3 from P19 nuclear extracts.

The PA1-884 immunogen is a synthetic peptide corresponding to residues 1-14 of mouse DNMT3b. This sequence is 79% conserved between human and mouse. PA1-884 immunizing peptide (Cat. # PEP-117) is available for use in neutralization and control experiments.

靶标信息

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Six alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

DNA demethylation of promoter region orchestrates SPI-1-induced ADAMTS-5 expression in articular cartilage of osteoarthritis mice.

Journal of cellular physiology

Liu Z,Lu T,Ma L,Zhang Y,Li D

Published figure using DNMT3B polyclonal antibody (Product # PA1-884) in Immunohistochemistry

Thu Feb 01 00:00:00 UTC 2024

DNA Methylation Dynamics During Esophageal Epithelial Regeneration Following Repair with Acellular Silk Fibroin Grafts in Rat.

Advanced biology

Urban LA,Li J,Gundogdu G,Trinh A,Shao H,Nguyen T,Mauney JR,Downing TL

PA1-884 was used in Immunohistochemistry to provide insight into the epigenetic mechanisms during esophageal regeneration following surgical repair with acellular grafts.

Mon May 01 00:00:00 UTC 2023

Dnmt3b catalytic activity is critical for its tumour suppressor function in lymphomagenesis and is associated with c-Met oncogenic signalling.

EBioMedicine

Lopusna K,Nowialis P,Opavska J,Abraham A,Riva A,Opavsky R

PA1-884 was used in Western Blot to study a role of Dnmt3b's catalytic activity in development and cancer using a mouse model expressing catalytically inactive Dnmt3b (Dnmt3bCI).

Fri Jan 01 00:00:00 UTC 2021

Dnmt3b catalytic activity is critical for its tumour suppressor function in lymphomagenesis and is associated with c-Met oncogenic signalling.

EBioMedicine

Lopusna K,Nowialis P,Opavska J,Abraham A,Riva A,Opavsky R

PA1-884 was used in Western Blot to study a role of Dnmt3b's catalytic activity in development and cancer using a mouse model expressing catalytically inactive Dnmt3b (Dnmt3bCI).

Fri Jan 01 00:00:00 UTC 2021

Epigenetic Regulation of CDH1 Is Altered after HOXB7-Silencing in MDA-MB-468 Triple-Negative Breast Cancer Cells.

Genes

Paço A,Leitão-Castro J,Freitas R

Published figure using DNMT3B polyclonal antibody (Product # PA1-884) in Western Blot

Sun Oct 03 00:00:00 UTC 2021

Epigenetic Regulation of CDH1 Is Altered after HOXB7-Silencing in MDA-MB-468 Triple-Negative Breast Cancer Cells.

Genes

Paço A,Leitão-Castro J,Freitas R

Published figure using DNMT3B polyclonal antibody (Product # PA1-884) in Western Blot

Sun Oct 03 00:00:00 UTC 2021

Decreases in different Dnmt3b activities drive distinct development of hematologic malignancies in mice.

The Journal of biological chemistry

Lopusna K,Nowialis P,Opavska J,Abraham A,Riva A,Haney SL,Opavsky R

Published figure using DNMT3B polyclonal antibody (Product # PA1-884) in Western Blot

Tue Aug 31 00:00:00 UTC 2021

Decreases in different Dnmt3b activities drive distinct development of hematologic malignancies in mice.

The Journal of biological chemistry

Lopusna K,Nowialis P,Opavska J,Abraham A,Riva A,Haney SL,Opavsky R

Published figure using DNMT3B polyclonal antibody (Product # PA1-884) in Western Blot

Tue Aug 31 00:00:00 UTC 2021

Catalytically inactive Dnmt3b rescues mouse embryonic development by accessory and repressive functions.

Nature communications

Nowialis P,Lopusna K,Opavska J,Haney SL,Abraham A,Sheng P,Riva A,Natarajan A,Guryanova O,Simpson M,Hlady R,Xie M,Opavsky R

PA1-884 was used in Western Blotting to highlight that Dnmt3b is a multifaceted protein that serves as an enzyme, an accessory factor for other methyltransferases, and as a transcriptional repressor in mouse embryogenesis.

Thu Sep 26 00:00:00 UTC 2019

Diabetes impairs wound healing by Dnmt1-dependent dysregulation of hematopoietic stem cells differentiation towards macrophages.

Nature communications

Yan J,Tie G,Wang S,Tutto A,DeMarco N,Khair L,Fazzio TG,Messina LM

PA1-884 was used in Western Blotting to demonstrate for the first time that type 2 diabetes mellitus induces an haematopoietic stem cell-autonomous mechanism that causes impaired wound healing.

Tue Jan 02 00:00:00 UTC 2018

The SNF2 family ATPase LSH promotes cell-autonomous de novo DNA methylation in somatic cells.

Nucleic acids research

Termanis A,Torrea N,Culley J,Kerr A,Ramsahoye B,Stancheva I

Published figure using DNMT3B polyclonal antibody (Product # PA1-884) in Western Blot

Mon Sep 19 00:00:00 UTC 2016

G9a/GLP Complex Maintains Imprinted DNA Methylation in Embryonic Stem Cells.

Cell reports

Zhang T,Termanis A,Özkan B,Bao XX,Culley J,de Lima Alves F,Rappsilber J,Ramsahoye B,Stancheva I

PA1-884 was used in western blot to characterize the G9a/GLP complex and how it maintains imprinted DNA methylation in embryonic stem cells

Tue Apr 05 00:00:00 UTC 2016

LSH and G9a/GLP complex are required for developmentally programmed DNA methylation.

Genome research

Myant K,Termanis A,Sundaram AY,Boe T,Li C,Merusi C,Burrage J,de Las Heras JI,Stancheva I

PA1-884 was used in western blot to investigate the role of LSH and G9a/GLP in DNA methylation during development

Sat Jan 01 00:00:00 UTC 2011

De novo DNA methyltransferase DNMT3b interacts with NEDD8-modified proteins.

The Journal of biological chemistry

Shamay M,Greenway M,Liao G,Ambinder RF,Hayward SD

PA1-884 was used in western blot to study the interaction between de novo DNA methyltransferase DNMT3b and NEDD8-modified proteins

Fri Nov 19 00:00:00 UTC 2010

LSH cooperates with DNA methyltransferases to repress transcription.

Molecular and cellular biology

Myant K,Stancheva I

PA1-884 was used in western blot to investigate the role of LSH during DNA methylation by DNA methyltransferases in mammals

Tue Jan 01 00:00:00 UTC 2008

生物信息学

蛋白别名: DNA (cytosine-5)-methyltransferase 3B; DNA (cytosine-5-)-methyltransferase 3 beta; DNA methyltransferase HsaIIIB; DNA methyltransferase MmuIIIB; DNA MTase HsaIIIB; DNA MTase MmuIIIB; Dnmt3; Dnmt3b; m.MmuIIIB; MGC1090

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基因别名: DNMT3B; ICF; ICF1; M.HsaIIIB; MmuIIIB

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UniProt ID: (Human) Q9UBC3, (Mouse) O88509

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Entrez Gene ID: (Human) 1789, (Mouse) 13436

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功能